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- W2085752436 abstract "A Leydig cell line, TTE1, has been established from transgenic mice harboring a temperature-sensitive simian virus 40 (tsSV40) large T-antigen gene. The cells grew at a permissive temperature (33 degrees C), but growth was markedly prevented at a nonpermissive temperature (39 degrees C). T-antigen was expressed in the nuclei at 33 degrees C but disappeared at 39 degrees C, indicating that the cells show a temperature-sensitive growth phenotype reflected by the tsSV40 large T-antigen. TTE1 cells did not show any colony-forming activity in soft agar and form tumors in subcutaneous tissue in nude mice, indicating that the cells were not transformed. Alkaline phosphatase and 3beta-hydroxysteroid dehydrogenase (HSD) activities or expression of cytokeratin and vimentin were observed. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that TTE1 cells expressed mRNAs encoding 17beta-HSD types 1 and 3, and inhibin-alpha. The cells with unique characteristics, therefore, should serve useful model study the function of Leydig cell." @default.
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- W2085752436 date "2002-01-01" @default.
- W2085752436 modified "2023-10-14" @default.
- W2085752436 title "ESTABLISHMENT OF LEYDIG CELL LINE, TTE1, FROM TRANSGENIC MICE HARBORING TEMPERATURE-SENSITIVE SIMIAN VIRUS 40 LARGE T-ANTIGEN GENE" @default.
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- W2085752436 doi "https://doi.org/10.1080/014850102753385206" @default.
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