Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085754322> ?p ?o ?g. }
- W2085754322 endingPage "3456" @default.
- W2085754322 startingPage "3436" @default.
- W2085754322 abstract "Heterogeneity of ADP diffusion and regulation of respiration were studied in permeabilized cardiomyocytes and cardiac fibers in situ and in silico. Regular arrangement of mitochondria in cells was altered by short-time treatment with trypsin and visualized by confocal microscopy. Manipulation of matrix volumes by changing K(+) and sucrose concentrations did not affect the affinity for ADP either in isolated heart mitochondria or in skinned fibers. Pyruvate kinase (PK)-phosphoenolpyruvate (PEP) were used to trap ADP generated in Ca,MgATPase reactions. Inhibition of respiration by PK-PEP increased 2-3 times after disorganization of regular mitochondrial arrangement in cells. ADP produced locally in the mitochondrial creatine kinase reaction was not accessible to PK-PEP in intact permeabilized fibers, but some part of it was released from mitochondria after short proteolysis due to increased permeability of outer mitochondrial membrane. In in silico studies we show by mathematical modeling that these results can be explained by heterogeneity of ADP diffusion due to its restrictions at the outer mitochondrial membrane and in close areas, which is changed after proteolysis. Localized restrictions and heterogeneity of ADP diffusion demonstrate the importance of mitochondrial functional complexes with sarcoplasmic reticulum and myofibrillar structures and creatine kinase in regulation of oxidative phosphorylation." @default.
- W2085754322 created "2016-06-24" @default.
- W2085754322 creator A5002203819 @default.
- W2085754322 creator A5005339099 @default.
- W2085754322 creator A5011405536 @default.
- W2085754322 creator A5021091266 @default.
- W2085754322 creator A5029224152 @default.
- W2085754322 creator A5043404598 @default.
- W2085754322 creator A5050013858 @default.
- W2085754322 creator A5055777045 @default.
- W2085754322 creator A5059064296 @default.
- W2085754322 creator A5068489758 @default.
- W2085754322 date "2003-05-01" @default.
- W2085754322 modified "2023-10-17" @default.
- W2085754322 title "Heterogeneity of ADP Diffusion and Regulation of Respiration in Cardiac Cells" @default.
- W2085754322 cites W1538627739 @default.
- W2085754322 cites W1542197083 @default.
- W2085754322 cites W1544928020 @default.
- W2085754322 cites W1553678646 @default.
- W2085754322 cites W1564575810 @default.
- W2085754322 cites W1661549035 @default.
- W2085754322 cites W1849159432 @default.
- W2085754322 cites W1935060748 @default.
- W2085754322 cites W1963715201 @default.
- W2085754322 cites W1968512837 @default.
- W2085754322 cites W1975357367 @default.
- W2085754322 cites W1976701283 @default.
- W2085754322 cites W1978278112 @default.
- W2085754322 cites W1979439323 @default.
- W2085754322 cites W1983090416 @default.
- W2085754322 cites W1987137066 @default.
- W2085754322 cites W1987584482 @default.
- W2085754322 cites W1992684611 @default.
- W2085754322 cites W1996907401 @default.
- W2085754322 cites W2004092450 @default.
- W2085754322 cites W2007552499 @default.
- W2085754322 cites W2010553753 @default.
- W2085754322 cites W2010670898 @default.
- W2085754322 cites W2024924175 @default.
- W2085754322 cites W2025635154 @default.
- W2085754322 cites W2028045661 @default.
- W2085754322 cites W2028436089 @default.
- W2085754322 cites W2034349571 @default.
- W2085754322 cites W2037097805 @default.
- W2085754322 cites W2040704590 @default.
- W2085754322 cites W2044726034 @default.
- W2085754322 cites W2046353863 @default.
- W2085754322 cites W2056814583 @default.
- W2085754322 cites W2064832125 @default.
- W2085754322 cites W2066987581 @default.
- W2085754322 cites W2069342809 @default.
- W2085754322 cites W2069867390 @default.
- W2085754322 cites W2072528174 @default.
- W2085754322 cites W2076802214 @default.
- W2085754322 cites W2077930835 @default.
- W2085754322 cites W2078132151 @default.
- W2085754322 cites W2079247552 @default.
- W2085754322 cites W2080312195 @default.
- W2085754322 cites W2085058400 @default.
- W2085754322 cites W2086661624 @default.
- W2085754322 cites W2089538133 @default.
- W2085754322 cites W2092135118 @default.
- W2085754322 cites W2092224990 @default.
- W2085754322 cites W2092301915 @default.
- W2085754322 cites W2115322996 @default.
- W2085754322 cites W2120382894 @default.
- W2085754322 cites W2130513658 @default.
- W2085754322 cites W2132079572 @default.
- W2085754322 cites W2144699036 @default.
- W2085754322 cites W2147607863 @default.
- W2085754322 cites W2147819622 @default.
- W2085754322 cites W2148191063 @default.
- W2085754322 cites W2162234541 @default.
- W2085754322 cites W2170597525 @default.
- W2085754322 cites W2171174995 @default.
- W2085754322 cites W2175290961 @default.
- W2085754322 cites W2184484397 @default.
- W2085754322 cites W2276148590 @default.
- W2085754322 cites W2281889878 @default.
- W2085754322 cites W2331706143 @default.
- W2085754322 cites W2338771681 @default.
- W2085754322 cites W2410892011 @default.
- W2085754322 cites W2462415355 @default.
- W2085754322 cites W247724665 @default.
- W2085754322 cites W267159847 @default.
- W2085754322 cites W274125308 @default.
- W2085754322 cites W3093453526 @default.
- W2085754322 cites W3150545440 @default.
- W2085754322 cites W4250737642 @default.
- W2085754322 cites W4250841709 @default.
- W2085754322 cites W4252205273 @default.
- W2085754322 cites W4254506477 @default.
- W2085754322 doi "https://doi.org/10.1016/s0006-3495(03)70065-4" @default.
- W2085754322 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1302901" @default.
- W2085754322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12719270" @default.
- W2085754322 hasPublicationYear "2003" @default.
- W2085754322 type Work @default.
- W2085754322 sameAs 2085754322 @default.