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- W2085754639 endingPage "148" @default.
- W2085754639 startingPage "137" @default.
- W2085754639 abstract "Lipids have been increasingly used as carriers for delivery of proteins and peptides. In this study, thermal stability and conformational properties of two basic proteins, ribonuclease A (RNase A) and cytochrome c (cyt. c), incorporated in lipid membranes made with dipalmitoylphosphatidylglycerol (DPPG), a negatively charged lipid, was studied by differential scanning calorimetry (DSC) and Fourier transform infrared (FT-IR) spectroscopy. DSC studies showed that when incorporated in DPPG at concentration <4 mol%, these two proteins were stabilized by DPPG binding, and >4 mol%, a destabilizing effect was observed. Such a decrease in thermal stability of cyt. c or RNase A suggested a strong intermolecular protein–protein interaction, because of the relatively low lipid to protein ratio. When cyt. c bound to membranes made of a mixture of DPPG and DPPC (dipalmitoylphosphatidylcholine), the extent of structural perturbation depended on the surface density of the negatively charged lipid head groups; perturbation became smaller with increasing acidic phospholipid in the membrane. FT-IR studies showed a shift of the major amide I component band from 1653 to 1649 cm−1 for cyt. c and from 1639 to 1633 cm−1 for RNase A after incorporation into DPPG membranes. However, from the quantitative determination of Fourier self-deconvoluted spectra, only slight perturbation of the secondary structure was observed after DPPG binding. These reductions, along with the shifts to lower frequencies of the main component bands, suggested that some rearrangement within the α helices/β sheets and/or the loosening of the protein tertiary structure existed, resulting in a stronger hydrogen bonding accessibility after their binding to DPPG membranes." @default.
- W2085754639 created "2016-06-24" @default.
- W2085754639 creator A5010147133 @default.
- W2085754639 creator A5042171311 @default.
- W2085754639 date "1998-02-01" @default.
- W2085754639 modified "2023-10-03" @default.
- W2085754639 title "Effect of lipids on the thermal stability and conformational changes of proteins: ribonuclease A and cytochrome c" @default.
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- W2085754639 doi "https://doi.org/10.1016/s0378-5173(97)00344-x" @default.
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