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• W2085756960 startingPage "16192" @default.
• W2085756960 abstract "We use single-molecule force spectroscopy to drive single GFP molecules from the native state through their complex energy landscape into the completely unfolded state. Unlike many smaller proteins, mechanical GFP unfolding proceeds by means of two subsequent intermediate states. The transition from the native state to the first intermediate state occurs near thermal equilibrium at ≈35 pN and is characterized by detachment of a seven-residue N-terminal α-helix from the beta barrel. We measure the equilibrium free energy cost associated with this transition as 22 k B T. Detachment of this small α-helix completely destabilizes GFP thermodynamically even though the β-barrel is still intact and can bear load. Mechanical stability of the protein on the millisecond timescale, however, is determined by the activation barrier of unfolding the β-barrel out of this thermodynamically unstable intermediate state. High bandwidth, time-resolved measurements of the cantilever relaxation phase upon unfolding of the β-barrel revealed a second metastable mechanical intermediate with one complete β-strand detached from the barrel. Quantitative analysis of force distributions and lifetimes lead to a detailed picture of the complex mechanical unfolding pathway through a rough energy landscape." @default.
• W2085756960 created "2016-06-24" @default.
• W2085756960 creator A5015801201 @default.
• W2085756960 creator A5072507626 @default.
• W2085756960 date "2004-11-05" @default.
• W2085756960 modified "2023-10-17" @default.
• W2085756960 title "Exploring the energy landscape of GFP by single-molecule mechanical experiments" @default.
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• W2085756960 doi "https://doi.org/10.1073/pnas.0404549101" @default.
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• W2085756960 hasPublicationYear "2004" @default.
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