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- W2085793934 abstract "Abstract 1. 1. Purified preparations of N4 bacteriophage were treated with cold phenol and the DNA was subjected to a series of physical and chemical investigations. 2. 2. The extracted DNA was shown to be homogeneous by ultracentrifugation and chromatography on magnesium pyrophosphate gel. 3. 3. The size of N4 DNA, as determined from sedimentation, viscometry and light-scattering studies, was found to be in the range of 40·106 daltons. Since the molecular weight of N4 bacteriophage is 83·106, there is probably only one DNA molecule per virus particle. 4. 4. Thermal denaturation studies provided evidence for a double-stranded secondary structure of N4 DNA. The transition temperature was 87.2° corresponding to a mean guanine plus cytosine content of 44 moles %. 5. 5. The buoyant density of native N4 DNA in CsCl density gradients was found to be 1.707 g/ml and an increase of 0.015 g/ml was observed for thermally denatured samples banded at the isopycnic point." @default.
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- W2085793934 date "1966-12-01" @default.
- W2085793934 modified "2023-09-25" @default.
- W2085793934 title "The physical properties of the deoxyribonucleic acid from N4 coliphage" @default.
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- W2085793934 doi "https://doi.org/10.1016/0005-2787(66)90064-5" @default.
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