FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085864013> ?p ?o ?g. }

• W2085864013 endingPage "382" @default.
• W2085864013 startingPage "351" @default.
• W2085864013 abstract "The increase in the number of deaths from infections caused by multidrug-resistant bacteria and cancer diseases highlights the need for new molecules with biological activity. Actinobacteria represent a potential source of new compounds, as these microorganisms have already produced a great diversity of clinically employed antibiotics. Endophytes from unexplored biomes, such as the Pantanal (the largest wetland in the world), can be a source of new molecules. Hymenachne amplexicaulis is among the unexplored native plants of the Pantanal in terms of its endophytic community. This plant is considered a weed in other countries due to its ability to adapt and compete with native plants, and there is evidence to suggest that the endophytic community of H. amplexicaulis plays an important role in this competitiveness. To explore its therapeutic potential, the present study isolated, identified (using partial sequence of the 16S rDNA) and bioprospected H. amplexicaulis endophytic actinobacteria. Ten isolates belonging to the genera Streptomyces, Microbispora, Leifsonia, and Verrucosispora were obtained from root fragments. The susceptibility profile of the isolates to the different classes of antibiotics was evaluated, with 80 % of the isolates showing resistance to the antibiotics Nalidixic Acid, Ampicillin, Chloramphenicol, Oxacillin, and Rifampicin. To assess antibacterial and antitumor activities, methanolic extracts were obtained by fermentation in SG culture medium at 36 °C at 180 rpm for 10 days. The extract produced from the S. albidoflavus CMRP4854 isolate was the only one to show activity against the Gram-negative bacterium Acinetobacter baumanii. Due to the great clinical importance of this pathogen and the difficulty in obtaining active compounds against it, the CMRP4854 isolate should be further investigated for the identification of active compounds and mode of action. We also emphasize the results obtained by the extract of the isolates Streptomyces albidoflavus CMRP4852 and Verrucosispora sp. CMRP4860 that presented antibacterial effect against Methicilin-resistant Staphylococcus aureus (MRSA) (MIC: 1.5 μg/mL and 13 μg/mL, respectively) and Vancomycin-resistant Enterococcus (VRE) (MIC: 40 μg/mL for both extracts). Extracts (200 μg/mL) of these two endophytes also showed selective cytotoxicity action against murine B16-F10 melanoma cells. However, the CMRP4852 extract also affected the density of normal cells. Due to these results, the crude extract of isolate CMRP4860 Verrucosispora sp., which was the only one that presented cytotoxicity and reduced cell density only in tumor cells, was selected for subsequent analysis involving scale-up fermentation of the CMRP4860 resulting in 9 fractions that were tested against both bacteria and tumor cells, with particular fractions showing promise and meriting further investigation. Taken together, the results of this study not only show for the first time that the endophytic community of H. amplexicaulis actinobacteria can produce secondary metabolites that potentially possess important antibacterial and cytotoxic properties, but also reinforce the pressing need to conserve biomes such as the Brazilian Pantanal." @default.
• W2085864013 created "2016-06-24" @default.
• W2085864013 creator A5060626717 @default.
• W2085864013 creator A5068380786 @default.
• W2085864013 creator A5087832965 @default.
• W2085864013 date "2013-08-01" @default.
• W2085864013 modified "2023-09-23" @default.
• W2085864013 title "Current management of melanoma" @default.
• W2085864013 cites W1540044642 @default.
• W2085864013 cites W1639979835 @default.
• W2085864013 cites W1819917384 @default.
• W2085864013 cites W1828940258 @default.
• W2085864013 cites W1842956333 @default.
• W2085864013 cites W1879616740 @default.
• W2085864013 cites W1889451488 @default.
• W2085864013 cites W1891974901 @default.
• W2085864013 cites W1908752382 @default.
• W2085864013 cites W1917632054 @default.
• W2085864013 cites W1927159838 @default.
• W2085864013 cites W1932474041 @default.
• W2085864013 cites W1951046932 @default.
• W2085864013 cites W1969505757 @default.
• W2085864013 cites W1971947883 @default.
• W2085864013 cites W1972254125 @default.
• W2085864013 cites W1972546494 @default.
• W2085864013 cites W1973433251 @default.
• W2085864013 cites W1978113416 @default.
• W2085864013 cites W1978263800 @default.
• W2085864013 cites W1979225169 @default.
• W2085864013 cites W1980628003 @default.
• W2085864013 cites W1985198734 @default.
• W2085864013 cites W1985424846 @default.
• W2085864013 cites W1986793246 @default.
• W2085864013 cites W1987887705 @default.
• W2085864013 cites W1989121777 @default.
• W2085864013 cites W1989966316 @default.
• W2085864013 cites W1990120792 @default.
• W2085864013 cites W1990123659 @default.
• W2085864013 cites W1990752233 @default.
• W2085864013 cites W1990808326 @default.
• W2085864013 cites W1991169492 @default.
• W2085864013 cites W1994231940 @default.
• W2085864013 cites W1994572966 @default.
• W2085864013 cites W1995973428 @default.
• W2085864013 cites W1997587606 @default.
• W2085864013 cites W1997817818 @default.
• W2085864013 cites W1999176719 @default.
• W2085864013 cites W2001161741 @default.
• W2085864013 cites W2004801835 @default.
• W2085864013 cites W2009250800 @default.
• W2085864013 cites W2009567390 @default.
• W2085864013 cites W2010366796 @default.
• W2085864013 cites W2010973586 @default.
• W2085864013 cites W2012075200 @default.
• W2085864013 cites W2015381709 @default.
• W2085864013 cites W2018125561 @default.
• W2085864013 cites W2018448933 @default.
• W2085864013 cites W2019457348 @default.
• W2085864013 cites W2020041324 @default.
• W2085864013 cites W2020708650 @default.
• W2085864013 cites W2020727013 @default.
• W2085864013 cites W2024434420 @default.
• W2085864013 cites W2027567030 @default.
• W2085864013 cites W2032094516 @default.
• W2085864013 cites W2033005592 @default.
• W2085864013 cites W2036219081 @default.
• W2085864013 cites W2037459514 @default.
• W2085864013 cites W2037622995 @default.
• W2085864013 cites W2038280196 @default.
• W2085864013 cites W2039561410 @default.
• W2085864013 cites W2043465976 @default.
• W2085864013 cites W2047010843 @default.
• W2085864013 cites W2049525098 @default.
• W2085864013 cites W2050488734 @default.
• W2085864013 cites W2051498550 @default.
• W2085864013 cites W2051506850 @default.
• W2085864013 cites W2052326786 @default.
• W2085864013 cites W2053096632 @default.
• W2085864013 cites W2055084054 @default.
• W2085864013 cites W2056754109 @default.
• W2085864013 cites W2060159046 @default.
• W2085864013 cites W2061734521 @default.
• W2085864013 cites W2062145108 @default.
• W2085864013 cites W2069280089 @default.
• W2085864013 cites W2070154408 @default.
• W2085864013 cites W2070208642 @default.
• W2085864013 cites W2070802441 @default.
• W2085864013 cites W2072185185 @default.
• W2085864013 cites W2075641683 @default.
• W2085864013 cites W2076100932 @default.
• W2085864013 cites W2080404550 @default.
• W2085864013 cites W2082931183 @default.
• W2085864013 cites W2083571420 @default.
• W2085864013 cites W2085109260 @default.
• W2085864013 cites W2086635837 @default.
• W2085864013 cites W2086804697 @default.
• W2085864013 cites W2088037555 @default.
• W2085864013 cites W2090763189 @default.

• next