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- W2085871663 abstract "RATIONALE: Major allergens are often quantified by assays using antibodies, however as allergens often exists as different isoforms, parts of these allergens may not be quantified, as the antibodies may not bind to all isoforms. In these experiments we have investigated several antibodies capability of binding to different isoforms of group 1 and 2, in Dermatophagoides pteronyssinus and Dermatophagoides farinae extract. METHODS: Mite extract were subjected to Iso Electric Focusing (IEF) by using an Offgel Fractionator to separate the isoforms. The instrument only separate with regard to pH, therefore we can only conclude on mutations which do have an effect on the pH. These fractions were analysed in rocket immuno electrophoresis using both poly and monospecific polyclonal antibodies (pAbs), and in specific ELISA assay and Biacore using monoclonal antibodies (mAbs). RESULTS: From the IEF of the two extracts, we were able to define the fractions where either group 1 or 2 were present using a polyclonal antibody raised towards the whole extract. This result was compared with the specific allergen ELISA results and Biacore data using mAbs. From these data we can conclude, that some mAbs can bind to a similar spectre of isoforms as the pAb. Further similar ratio between the different isoforms was detected with both pAbs and mAbs. CONCLUSIONS: We were able to identify, that the monoclonal antibody tested could identify the same range of isoforms as the polyclonal antibody. Further the pAb and mAbs assays correlated well with regard to level of these isoforms." @default.
- W2085871663 created "2016-06-24" @default.
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- W2085871663 date "2011-02-01" @default.
- W2085871663 modified "2023-09-27" @default.
- W2085871663 title "Determination Of Group 1 and 2 Isoforms From House Dust Mite Using Different Antibody Based Assays" @default.
- W2085871663 doi "https://doi.org/10.1016/j.jaci.2010.12.670" @default.
- W2085871663 hasPublicationYear "2011" @default.
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