Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085983043> ?p ?o ?g. }
- W2085983043 endingPage "355" @default.
- W2085983043 startingPage "348" @default.
- W2085983043 abstract "A detailed protocol is presented for precisely inserting microscopic objects into the periplasmic region of plant callus cells using laser microsurgery. Ginkgo biloba and Agrobacterium rhizogenes were used as the model system for developing the optical tweezers and scalpel techniques using a single laser. We achieved better than 95% survival after plasmolyzing G. biloba cells, ablating a 2–4-μm hole through the cell wall using a pulsed UV laser beam, trapping and translating bacteria into the periplasmic region using a pulsed infrared laser beam, and then deplasmolyzing the cells. Insertion of bacteria is also described. A thermal model for temperature changes of trapped bacteria is included. Comparisons with other methods, such as a reverse-pressure gradient technique, are discussed and additional experiments on plants using laser microsurgery are suggested. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 348–355, 1998." @default.
- W2085983043 created "2016-06-24" @default.
- W2085983043 creator A5006231750 @default.
- W2085983043 creator A5046417109 @default.
- W2085983043 creator A5076429754 @default.
- W2085983043 creator A5091433248 @default.
- W2085983043 date "1998-11-05" @default.
- W2085983043 modified "2023-10-14" @default.
- W2085983043 title "Insertion of microscopic objects through plant cell walls using laser microsurgery" @default.
- W2085983043 cites W1594330113 @default.
- W2085983043 cites W194650631 @default.
- W2085983043 cites W1967118561 @default.
- W2085983043 cites W1972940444 @default.
- W2085983043 cites W1976990911 @default.
- W2085983043 cites W1979340466 @default.
- W2085983043 cites W1983251735 @default.
- W2085983043 cites W1987528799 @default.
- W2085983043 cites W1990388601 @default.
- W2085983043 cites W1995636525 @default.
- W2085983043 cites W1997231573 @default.
- W2085983043 cites W2007000423 @default.
- W2085983043 cites W2009327252 @default.
- W2085983043 cites W2011089170 @default.
- W2085983043 cites W2011573266 @default.
- W2085983043 cites W2022817606 @default.
- W2085983043 cites W2026311140 @default.
- W2085983043 cites W2030365976 @default.
- W2085983043 cites W2036442731 @default.
- W2085983043 cites W2060153029 @default.
- W2085983043 cites W2061053738 @default.
- W2085983043 cites W2066928538 @default.
- W2085983043 cites W2069176961 @default.
- W2085983043 cites W2071195062 @default.
- W2085983043 cites W2092164769 @default.
- W2085983043 cites W2094144546 @default.
- W2085983043 cites W2105600671 @default.
- W2085983043 cites W2108461844 @default.
- W2085983043 cites W2113372755 @default.
- W2085983043 cites W2133220149 @default.
- W2085983043 cites W2136954365 @default.
- W2085983043 cites W2142180764 @default.
- W2085983043 cites W2164431873 @default.
- W2085983043 doi "https://doi.org/10.1002/(sici)1097-0290(19981105)60:3<348::aid-bit11>3.0.co;2-i" @default.
- W2085983043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10099438" @default.
- W2085983043 hasPublicationYear "1998" @default.
- W2085983043 type Work @default.
- W2085983043 sameAs 2085983043 @default.
- W2085983043 citedByCount "20" @default.
- W2085983043 countsByYear W20859830432012 @default.
- W2085983043 countsByYear W20859830432013 @default.
- W2085983043 countsByYear W20859830432022 @default.
- W2085983043 crossrefType "journal-article" @default.
- W2085983043 hasAuthorship W2085983043A5006231750 @default.
- W2085983043 hasAuthorship W2085983043A5046417109 @default.
- W2085983043 hasAuthorship W2085983043A5076429754 @default.
- W2085983043 hasAuthorship W2085983043A5091433248 @default.
- W2085983043 hasConcept C104317684 @default.
- W2085983043 hasConcept C120665830 @default.
- W2085983043 hasConcept C121332964 @default.
- W2085983043 hasConcept C12554922 @default.
- W2085983043 hasConcept C185592680 @default.
- W2085983043 hasConcept C192562407 @default.
- W2085983043 hasConcept C201663137 @default.
- W2085983043 hasConcept C20198109 @default.
- W2085983043 hasConcept C204241405 @default.
- W2085983043 hasConcept C2778998842 @default.
- W2085983043 hasConcept C2779656422 @default.
- W2085983043 hasConcept C2984025587 @default.
- W2085983043 hasConcept C520434653 @default.
- W2085983043 hasConcept C523546767 @default.
- W2085983043 hasConcept C54355233 @default.
- W2085983043 hasConcept C547475151 @default.
- W2085983043 hasConcept C55493867 @default.
- W2085983043 hasConcept C59822182 @default.
- W2085983043 hasConcept C86803240 @default.
- W2085983043 hasConcept C9549007 @default.
- W2085983043 hasConceptScore W2085983043C104317684 @default.
- W2085983043 hasConceptScore W2085983043C120665830 @default.
- W2085983043 hasConceptScore W2085983043C121332964 @default.
- W2085983043 hasConceptScore W2085983043C12554922 @default.
- W2085983043 hasConceptScore W2085983043C185592680 @default.
- W2085983043 hasConceptScore W2085983043C192562407 @default.
- W2085983043 hasConceptScore W2085983043C201663137 @default.
- W2085983043 hasConceptScore W2085983043C20198109 @default.
- W2085983043 hasConceptScore W2085983043C204241405 @default.
- W2085983043 hasConceptScore W2085983043C2778998842 @default.
- W2085983043 hasConceptScore W2085983043C2779656422 @default.
- W2085983043 hasConceptScore W2085983043C2984025587 @default.
- W2085983043 hasConceptScore W2085983043C520434653 @default.
- W2085983043 hasConceptScore W2085983043C523546767 @default.
- W2085983043 hasConceptScore W2085983043C54355233 @default.
- W2085983043 hasConceptScore W2085983043C547475151 @default.
- W2085983043 hasConceptScore W2085983043C55493867 @default.
- W2085983043 hasConceptScore W2085983043C59822182 @default.
- W2085983043 hasConceptScore W2085983043C86803240 @default.
- W2085983043 hasConceptScore W2085983043C9549007 @default.
- W2085983043 hasIssue "3" @default.
- W2085983043 hasLocation W20859830431 @default.