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• W2085983265 abstract "This work reported a label-free colorimetric assay for sensitive detection of Hg2+ based on Hg2+-triggered hairpin DNA probe (H-DNA) termini-binding and exonuclease Ш (Exo Ш)-assisted target recycling, as well as hemin/G-quadruplex (DNAzyme) signal amplification. The specific binding of free Hg2+ with the thymine–thymine (T–T) mismatches termini of H-DNA could immediately trigger the Exo Ш digestion, and then set free G-quadruplex segments and Hg2+. The Exo Ш impellent recycling of ultratrace Hg2+ produced numerous G-quadruplexes. The corresponding DNAzymes catalyzed efficiently the H2O2-mediated oxidation of the ABTS2− to the colored product in the presence of hemin. Using the color change as the output signal, and the Exo Ш-aided Hg2+ recycling and DNAzyme as the signal amplifier, the ultrasensitive assay system successfully achieved visual detection of Hg2+ as low as 1.0 nM by the naked eye, and was suitable for field monitoring. The calibration curve was linear in the range of 50.0 pM to 20.0 nM for Hg2+ (R=0.9962) with a detection limit of 10.0 pM. Moreover, this proposed strategy showed excellent selectivity, portability and low-cost, and was successfully applied to colorimetric detection of Hg2+ in laboratory tap water and Jialing river water samples." @default.
• W2085983265 created "2016-06-24" @default.
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• W2085983265 date "2015-06-01" @default.
• W2085983265 modified "2023-09-24" @default.
• W2085983265 title "Label-free colorimetric detection of Hg 2+ based on Hg 2+ -triggered exonuclease III-assisted target recycling and DNAzyme amplification" @default.
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• W2085983265 doi "https://doi.org/10.1016/j.bios.2015.01.010" @default.
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