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• W2085984837 abstract "Surface properties of polymeric devices that are used to regenerate nervous damage are a point to be considered for axon regeneration in nerve system. In our previous studies, we prepared a wettability gradient on polyethylene (PE) surfaces using a corona discharge treatment from a knife-type electrode whose power increases gradually along the sample length. The PE surfaces were oxidized gradually with increasing power. The effect of surface wettability on the different types of cells has an important role for cell adhesion and proliferation. The purpose of this study is to investigate neurite formation on polymer surfaces with different wettability. Induction and growth of neurites from the rat pheochromocytoma (PC-12) cells attached on the polymer surfaces with different hydrophilicity were investigated using the wettability gradient PE surfaces prepared by a corona discharge treatment. Neurites were investigated for number and length of neurites in terms of surface wettability. It was observed that neurite formation of PC-12 cells was increased more onto the positions with moderate hydrophilicity of the wettability gradient surface than onto the more hydrophobic or hydrophilic positions. From those results, it could be assumed that initial adhesion of PC-12 cells was caused by more calf serum (CS) protein than nerve growth factor (NGF), whereas the neurite formation of PC-12 cells was caused by more NGF than CS protein. It follows from what has been said thus far that PC-12 cells are a differentiated neuronal phenotype with a long neurite at around the position 2.5 cm (water contact angle of about 55 deg). In conclusion, surface wettability plays an important role for neurite formation on the polymer surfaces for axon regeneration." @default.
• W2085984837 created "2016-06-24" @default.
• W2085984837 creator A5033290870 @default.
• W2085984837 creator A5048459296 @default.
• W2085984837 creator A5055094328 @default.
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• W2085984837 date "2003-03-01" @default.
• W2085984837 modified "2023-10-14" @default.
• W2085984837 title "The effect of surface wettability on induction and growth of neurites from the PC-12 cell on a polymer surface" @default.
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• W2085984837 doi "https://doi.org/10.1016/s0021-9797(02)00163-7" @default.
• W2085984837 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16256501" @default.
• W2085984837 hasPublicationYear "2003" @default.
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