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• W2085996909 abstract "The self-organization of dorsally-directed microtubules during cortical rotation in the Xenopus egg is essential for dorsal axis formation. The mechanisms controlling this process have been problematic to analyze, owing to difficulties in visualizing microtubules in living egg. Also, the order of events occurring at the onset of cortical rotation have not been satisfactorily visualized in vivo and have been inferred from staged fixed samples. To address these issues, we have characterized the dynamics of total microtubule and plus end behavior continuously throughout cortical rotation, as well as in oocytes and unfertilized eggs. Here, we show that the nascent microtubule network forms in the cortex but associates with the deep cytoplasm at the start of rotation. Importantly, plus ends remain cortical and become increasingly more numerous and active prior to rotation, with dorsal polarization occurring rapidly after the onset of rotation. Additionally, we show that vegetally localized Trim36 is required to attenuate dynamic plus end growth, suggesting that vegetal factors are needed to locally coordinate growth in the cortex." @default.
• W2085996909 created "2016-06-24" @default.
• W2085996909 creator A5002295904 @default.
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• W2085996909 date "2015-05-01" @default.
• W2085996909 modified "2023-09-24" @default.
• W2085996909 title "The dynamics of plus end polarization and microtubule assembly during Xenopus cortical rotation" @default.
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• W2085996909 doi "https://doi.org/10.1016/j.ydbio.2015.01.028" @default.
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