FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086000673> ?p ?o ?g. }

• W2086000673 endingPage "A12" @default.
• W2086000673 startingPage "A12" @default.
• W2086000673 abstract "microRNA (miRNA) genes transcribed by RNA polymerase II generate small noncoding miRNAs of 18 to 24 nucleotides after maturation process. The mature miRNAs and their associated isomirs specifically bind to different mRNA transcripts, resulting in down regulation of multiple genes within the cell in a highly multiplexed way. miRNA expression profiles differ between human cell types suggesting cell-specific impacts of each miRNA on the regulation of different biological processes. Comparison of miRNA profiles of tumor samples and adjacent normal tissues showed that some miRNAs are up- or down- regulated and suggested their implication during tumor progression. However, such a miRNA profiling approach is not sufficient to identify the respective role of each miRNA gene during the tumorigenesis.Here, to assess the individual role of each miRNA gene and its different isomirs in a specific cell environment, we have constructed a lentiviral miRNA expression library containing more than 1100 human known and novel miRNA precursors. The arrayed layout of our library allowed high-throughput screens with a large spectrum of functional read-outs using either normal or tumor cells. To exemplify this approach, the results of three different screens will be presented; i.e. identification of miRNAs that inhibit the BRAF pathway, miRNAs that inhibit tumor angiogenesis and miRNAs that stimulate the mesenchymal to epithelial transition. In addition, beyond this hit identification step, we will present detailed characterization of the role of the identified miRNAs in tumor progression by means of molecular and cellular functional assays.Combining our unique miRNA expression library with a functional screening platform has allowed the identification and the further characterization of several miRNAs able to significantly impact on tumor behavior supporting the therapeutic interest of some candidates.Citation Format: Paula I. van Noort, Negar Babae, Gerald W. Verhaegh, Willemijn M. Gommans, Francesco Cerisoli, Mark Verheul, Raymond M. Schiffelers, Arjan W. Griffioen, Jack A. Schalken, Eugene Berezikov, Edwin Cuppen, Roel Q. J. Schaapveld, Jos B. Poell, Gregoire P. Prevost, Meriem Bourajjaj, Suzanna Vidic, Judy R. van Beijnum, Rick J. van Haastert, Iman Schultz, Thijs de Gunt, Onno van Hooij. Identification of microRNA-based therapeutic candidates using a unique lentiviral microRNA overexpression library [abstract]. In: Proceedings of the AACR Special Conference on Noncoding RNAs and Cancer; 2012 Jan 8-11; Miami Beach, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(2 Suppl):Abstract nr A12." @default.
• W2086000673 created "2016-06-24" @default.
• W2086000673 creator A5002357139 @default.
• W2086000673 creator A5002549367 @default.
• W2086000673 creator A5008179774 @default.
• W2086000673 creator A5011288026 @default.
• W2086000673 creator A5014953739 @default.
• W2086000673 creator A5016556857 @default.
• W2086000673 creator A5018153072 @default.
• W2086000673 creator A5018911558 @default.
• W2086000673 creator A5024263446 @default.
• W2086000673 creator A5025142700 @default.
• W2086000673 creator A5037043919 @default.
• W2086000673 creator A5037926880 @default.
• W2086000673 creator A5044217419 @default.
• W2086000673 creator A5060302039 @default.
• W2086000673 creator A5063343357 @default.
• W2086000673 creator A5072084304 @default.
• W2086000673 creator A5075016435 @default.
• W2086000673 creator A5075683646 @default.
• W2086000673 creator A5080749424 @default.
• W2086000673 creator A5083387415 @default.
• W2086000673 creator A5088748023 @default.
• W2086000673 date "2012-01-08" @default.
• W2086000673 modified "2023-10-16" @default.
• W2086000673 title "Abstract A12: Identification of microRNA-based therapeutic candidates using a unique lentiviral microRNA overexpression library" @default.
• W2086000673 doi "https://doi.org/10.1158/1538-7445.nonrna12-a12" @default.
• W2086000673 hasPublicationYear "2012" @default.
• W2086000673 type Work @default.
• W2086000673 sameAs 2086000673 @default.
• W2086000673 citedByCount "0" @default.
• W2086000673 crossrefType "journal-article" @default.
• W2086000673 hasAuthorship W2086000673A5002357139 @default.
• W2086000673 hasAuthorship W2086000673A5002549367 @default.
• W2086000673 hasAuthorship W2086000673A5008179774 @default.
• W2086000673 hasAuthorship W2086000673A5011288026 @default.
• W2086000673 hasAuthorship W2086000673A5014953739 @default.
• W2086000673 hasAuthorship W2086000673A5016556857 @default.
• W2086000673 hasAuthorship W2086000673A5018153072 @default.
• W2086000673 hasAuthorship W2086000673A5018911558 @default.
• W2086000673 hasAuthorship W2086000673A5024263446 @default.
• W2086000673 hasAuthorship W2086000673A5025142700 @default.
• W2086000673 hasAuthorship W2086000673A5037043919 @default.
• W2086000673 hasAuthorship W2086000673A5037926880 @default.
• W2086000673 hasAuthorship W2086000673A5044217419 @default.
• W2086000673 hasAuthorship W2086000673A5060302039 @default.
• W2086000673 hasAuthorship W2086000673A5063343357 @default.
• W2086000673 hasAuthorship W2086000673A5072084304 @default.
• W2086000673 hasAuthorship W2086000673A5075016435 @default.
• W2086000673 hasAuthorship W2086000673A5075683646 @default.
• W2086000673 hasAuthorship W2086000673A5080749424 @default.
• W2086000673 hasAuthorship W2086000673A5083387415 @default.
• W2086000673 hasAuthorship W2086000673A5088748023 @default.
• W2086000673 hasConcept C104317684 @default.
• W2086000673 hasConcept C119056186 @default.
• W2086000673 hasConcept C145059251 @default.
• W2086000673 hasConcept C150194340 @default.
• W2086000673 hasConcept C165864922 @default.
• W2086000673 hasConcept C18431079 @default.
• W2086000673 hasConcept C54355233 @default.
• W2086000673 hasConcept C555283112 @default.
• W2086000673 hasConcept C70721500 @default.
• W2086000673 hasConcept C86803240 @default.
• W2086000673 hasConceptScore W2086000673C104317684 @default.
• W2086000673 hasConceptScore W2086000673C119056186 @default.
• W2086000673 hasConceptScore W2086000673C145059251 @default.
• W2086000673 hasConceptScore W2086000673C150194340 @default.
• W2086000673 hasConceptScore W2086000673C165864922 @default.
• W2086000673 hasConceptScore W2086000673C18431079 @default.
• W2086000673 hasConceptScore W2086000673C54355233 @default.
• W2086000673 hasConceptScore W2086000673C555283112 @default.
• W2086000673 hasConceptScore W2086000673C70721500 @default.
• W2086000673 hasConceptScore W2086000673C86803240 @default.
• W2086000673 hasIssue "2 Supplement" @default.
• W2086000673 hasLocation W20860006731 @default.
• W2086000673 hasOpenAccess W2086000673 @default.
• W2086000673 hasPrimaryLocation W20860006731 @default.
• W2086000673 hasRelatedWork W1582164927 @default.
• W2086000673 hasRelatedWork W159859670 @default.
• W2086000673 hasRelatedWork W2026528414 @default.
• W2086000673 hasRelatedWork W2058930541 @default.
• W2086000673 hasRelatedWork W2065802121 @default.
• W2086000673 hasRelatedWork W2072111454 @default.
• W2086000673 hasRelatedWork W2088738576 @default.
• W2086000673 hasRelatedWork W2100084025 @default.
• W2086000673 hasRelatedWork W2161283857 @default.
• W2086000673 hasRelatedWork W2298589276 @default.
• W2086000673 hasRelatedWork W2327700253 @default.
• W2086000673 hasRelatedWork W2334153544 @default.
• W2086000673 hasRelatedWork W2366692242 @default.
• W2086000673 hasRelatedWork W2378466603 @default.
• W2086000673 hasRelatedWork W2384347487 @default.
• W2086000673 hasRelatedWork W3029345193 @default.
• W2086000673 hasRelatedWork W3045915179 @default.
• W2086000673 hasRelatedWork W3083368552 @default.
• W2086000673 hasRelatedWork W2140942829 @default.
• W2086000673 hasRelatedWork W2184788651 @default.
• W2086000673 hasVolume "72" @default.

• next