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• W2086022971 abstract "A method, using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for analyzing chitin oligosaccharides is described. Chitin oligosaccharides were derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) via reductive amination at 37 degrees C for 16 h (optimized conditions). The APTS-chitin oligosaccharides were analyzed using either an acidic citric acid-phosphate buffer or an alkaline borate buffer. The effects of buffer types, buffer pH values, and buffer concentrations on the separation were examined. The analytes were successfully separated by using a pH 4.6 citric acid-phosphate within 19 min. The APTS-derivatized chitin monosaccharide (D-glucosamine) migrated first. The analytes were also completely separated by using a pH 9.0 borate buffer within 24 min. Moreover, the specificity of enzyme digestion on chitin polysaccharides using the optimized APTS labeling procedure and the CE-LIF method was demonstrated." @default.
• W2086022971 created "2016-06-24" @default.
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• W2086022971 date "2002-12-01" @default.
• W2086022971 modified "2023-10-14" @default.
• W2086022971 title "Analysis of chitin oligosaccharides by capillary electrophoresis with laser-induced fluorescence" @default.
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• W2086022971 doi "https://doi.org/10.1016/s0021-9673(02)01260-8" @default.
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