FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086035863> ?p ?o ?g. }

• W2086035863 endingPage "546" @default.
• W2086035863 startingPage "537" @default.
• W2086035863 abstract "Troponin C (TnC) from amphioxus (Protochordate) was purified and its primary structure determined. Unlike the case of vertebrates and other invertebrates, amphioxus TnC is found in the soluble fraction after extractions at physiological ionic strength in the presence of Ca2+. Edman sequencing combined with mass spectroscopy indicate that the protein contains 163 amino acid residues. It possesses an acetylated N-terminus (although a small percentage has a free Ser N-terminus) and either ɛ-N-methyllysine or ɛ-N-dimethyllysine in position 20. It displays about 50% sequence identity with vertebrate skeletal-muscle and cardiac-muscle TnC, 44% with TnC of sea squirt, also a Protochordate, and 30% with other invertebrate TnC. Like vertebrate TnC, amphioxus TnC contains a N-terminal α-helix plus the usual four ancestral Ca2+-binding regions, but analysis of the sequence suggests that the fourth site is not functional. Flow dialysis shows that amphioxus TnC binds three Ca2+ with the mean apparent affinity constant K′ of 3.4 ± 1.5 105 M−1. No cooperativity exists between the sites, and the presence of up to 10 mM Mg2+ does not influence the Ca2+-binding isotherm, indicating that the metal-binding sites are Ca2+-specific at physiological Mg2+ concentrations. It forms a Ca2+-dependent, 1:1 complex with melittin and rabbit or crayfish troponin I (TnI). Amphioxus TnC possesses one Trp residue in position 151 and one at the C-terminus. Trp fluorescence suggests that one or both residues are solvent-exposed in the metal-free form and efficiently shielded in the Ca2+ form. Although Mg2+ has no effect on the Ca2+ binding, the Trp fluorescence is influenced by millimolar Mg2+, suggesting the presence of one or more independent Mg2+-binding site(s). A phylogenetic analysis clearly shows that amphioxus TnC is positioned on the branch of the Chordates, but at a distance from the vertebrate TnC. Its place on the phylogenetic tree is in accordance with the consensus evolutionary phylogeny." @default.
• W2086035863 created "2016-06-24" @default.
• W2086035863 creator A5012494228 @default.
• W2086035863 creator A5013246094 @default.
• W2086035863 creator A5028337407 @default.
• W2086035863 creator A5029106499 @default.
• W2086035863 creator A5055723850 @default.
• W2086035863 creator A5080510384 @default.
• W2086035863 date "1994-04-01" @default.
• W2086035863 modified "2023-09-24" @default.
• W2086035863 title "Characterization and primary structure of amphioxus troponin C" @default.
• W2086035863 cites W1489224259 @default.
• W2086035863 cites W1489435666 @default.
• W2086035863 cites W1504005082 @default.
• W2086035863 cites W1513843435 @default.
• W2086035863 cites W1524101826 @default.
• W2086035863 cites W1539344861 @default.
• W2086035863 cites W1550330933 @default.
• W2086035863 cites W1571243084 @default.
• W2086035863 cites W1578284467 @default.
• W2086035863 cites W1580591262 @default.
• W2086035863 cites W1587148368 @default.
• W2086035863 cites W1587627399 @default.
• W2086035863 cites W1598771652 @default.
• W2086035863 cites W1607301385 @default.
• W2086035863 cites W1861602137 @default.
• W2086035863 cites W1885844626 @default.
• W2086035863 cites W188730109 @default.
• W2086035863 cites W1900935399 @default.
• W2086035863 cites W1974377070 @default.
• W2086035863 cites W1974980877 @default.
• W2086035863 cites W1975475509 @default.
• W2086035863 cites W1985322317 @default.
• W2086035863 cites W1989295141 @default.
• W2086035863 cites W2005283875 @default.
• W2086035863 cites W2009004112 @default.
• W2086035863 cites W2012675680 @default.
• W2086035863 cites W2022114235 @default.
• W2086035863 cites W2029414149 @default.
• W2086035863 cites W2035446727 @default.
• W2086035863 cites W2047250295 @default.
• W2086035863 cites W2047298002 @default.
• W2086035863 cites W2057976520 @default.
• W2086035863 cites W2060467524 @default.
• W2086035863 cites W2065087790 @default.
• W2086035863 cites W2074231493 @default.
• W2086035863 cites W2085905607 @default.
• W2086035863 cites W2100837269 @default.
• W2086035863 cites W2110591420 @default.
• W2086035863 cites W2128635872 @default.
• W2086035863 cites W2140015409 @default.
• W2086035863 cites W2253286196 @default.
• W2086035863 cites W2326879084 @default.
• W2086035863 cites W2341772994 @default.
• W2086035863 cites W4243976693 @default.
• W2086035863 cites W4248004744 @default.
• W2086035863 doi "https://doi.org/10.1111/j.1432-1033.1994.tb18766.x" @default.
• W2086035863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8168542" @default.
• W2086035863 hasPublicationYear "1994" @default.
• W2086035863 type Work @default.
• W2086035863 sameAs 2086035863 @default.
• W2086035863 citedByCount "15" @default.
• W2086035863 countsByYear W20860358632013 @default.
• W2086035863 crossrefType "journal-article" @default.
• W2086035863 hasAuthorship W2086035863A5012494228 @default.
• W2086035863 hasAuthorship W2086035863A5013246094 @default.
• W2086035863 hasAuthorship W2086035863A5028337407 @default.
• W2086035863 hasAuthorship W2086035863A5029106499 @default.
• W2086035863 hasAuthorship W2086035863A5055723850 @default.
• W2086035863 hasAuthorship W2086035863A5080510384 @default.
• W2086035863 hasConcept C104317684 @default.
• W2086035863 hasConcept C12554922 @default.
• W2086035863 hasConcept C126322002 @default.
• W2086035863 hasConcept C133927893 @default.
• W2086035863 hasConcept C166014724 @default.
• W2086035863 hasConcept C167625842 @default.
• W2086035863 hasConcept C185592680 @default.
• W2086035863 hasConcept C36036425 @default.
• W2086035863 hasConcept C500558357 @default.
• W2086035863 hasConcept C55493867 @default.
• W2086035863 hasConcept C71240020 @default.
• W2086035863 hasConcept C71924100 @default.
• W2086035863 hasConcept C86803240 @default.
• W2086035863 hasConcept C87933860 @default.
• W2086035863 hasConcept C92825506 @default.
• W2086035863 hasConceptScore W2086035863C104317684 @default.
• W2086035863 hasConceptScore W2086035863C12554922 @default.
• W2086035863 hasConceptScore W2086035863C126322002 @default.
• W2086035863 hasConceptScore W2086035863C133927893 @default.
• W2086035863 hasConceptScore W2086035863C166014724 @default.
• W2086035863 hasConceptScore W2086035863C167625842 @default.
• W2086035863 hasConceptScore W2086035863C185592680 @default.
• W2086035863 hasConceptScore W2086035863C36036425 @default.
• W2086035863 hasConceptScore W2086035863C500558357 @default.
• W2086035863 hasConceptScore W2086035863C55493867 @default.
• W2086035863 hasConceptScore W2086035863C71240020 @default.
• W2086035863 hasConceptScore W2086035863C71924100 @default.
• W2086035863 hasConceptScore W2086035863C86803240 @default.

• next