FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086038644> ?p ?o ?g. }

• W2086038644 abstract "Chinese hamster ovary (CHO) cells in the plateau phase were seeded in the absence or presence of 5 mM 2-difluoromethylornithine (F2MeOrn), an enzyme-activated irreversible inhibitor of ornithine decarboxylase. The thymidine analogue bromodeoxyuridine (BrdUrd, 5 microM) was added to the culture medium 30 min before sampling of the cells, which occurred 1-17 h after seeding. Using flow cytometry, coupled with an indirect immunofluorescence technique, which utilized monoclonal BrdUrd and secondary fluorescein-isothiocyanate-conjugated antibodies, and the DNA stain propidium iodide, cellular BrdUrd and DNA contents were quantified. To determine if there was a perturbation in the progression of cells through the S phase, the distribution of BrdUrd-labelled cells in the S phase was evaluated in two ways: (a) by calculating the mean DNA content of BrdUrd-labelled cells in relation to the mean DNA contents of G1 and G2 cells (relative movementzero) and (b) by studying DNA histograms of BrdUrd-labelled cells. By using both evaluation methods, we show that DNA replication was impaired during the first cell cycle that was initiated after seeding CHO cells in the presence of F2MeOrn. The cells appeared to enter the S phase normally but were then delayed in their progression through this phase. The impairment of F2MeOrn treatment on DNA replication was apparent at 9 h after seeding, a time point at which the putrescine pool was depleted, the spermidine pool was approximately halved, and the spermine pool was unaffected, when compared to corresponding pools of control cells. When cells were seeded in the presence of F2MeOrn and putrescine, the effect on DNA replication was prevented. The rates of incorporation of [3H]uridine and [3H]leucine into RNA and protein, respectively, were the same in control and in F2MeOrn-treated cells for at least up to 11 h after seeding." @default.
• W2086038644 created "2016-06-24" @default.
• W2086038644 creator A5003182142 @default.
• W2086038644 creator A5048057596 @default.
• W2086038644 date "1996-05-01" @default.
• W2086038644 modified "2023-09-27" @default.
• W2086038644 title "Impairment of DNA Replication within One Cell Cycle after Seeding of Cells in the Presence of a Polyamine-Biosynthesis Inhibitor" @default.
• W2086038644 cites W152143163 @default.
• W2086038644 cites W1546975843 @default.
• W2086038644 cites W1564441910 @default.
• W2086038644 cites W1806852508 @default.
• W2086038644 cites W1975509011 @default.
• W2086038644 cites W1980334365 @default.
• W2086038644 cites W1990974074 @default.
• W2086038644 cites W1995397953 @default.
• W2086038644 cites W2000179569 @default.
• W2086038644 cites W2008228601 @default.
• W2086038644 cites W2029892599 @default.
• W2086038644 cites W2032448671 @default.
• W2086038644 cites W2035545032 @default.
• W2086038644 cites W2043985253 @default.
• W2086038644 cites W2044361096 @default.
• W2086038644 cites W2045553639 @default.
• W2086038644 cites W2070506598 @default.
• W2086038644 cites W2073316414 @default.
• W2086038644 cites W2076660768 @default.
• W2086038644 cites W2083079475 @default.
• W2086038644 cites W2083729530 @default.
• W2086038644 cites W2086528836 @default.
• W2086038644 cites W2092657673 @default.
• W2086038644 cites W2127799018 @default.
• W2086038644 cites W2203934997 @default.
• W2086038644 cites W2281124822 @default.
• W2086038644 cites W2341581173 @default.
• W2086038644 doi "https://doi.org/10.1111/j.1432-1033.1996.0539p.x" @default.
• W2086038644 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8647096" @default.
• W2086038644 hasPublicationYear "1996" @default.
• W2086038644 type Work @default.
• W2086038644 sameAs 2086038644 @default.
• W2086038644 citedByCount "27" @default.
• W2086038644 countsByYear W20860386442015 @default.
• W2086038644 countsByYear W20860386442021 @default.
• W2086038644 crossrefType "journal-article" @default.
• W2086038644 hasAuthorship W2086038644A5003182142 @default.
• W2086038644 hasAuthorship W2086038644A5048057596 @default.
• W2086038644 hasBestOaLocation W20860386441 @default.
• W2086038644 hasConcept C1491633281 @default.
• W2086038644 hasConcept C153911025 @default.
• W2086038644 hasConcept C175656101 @default.
• W2086038644 hasConcept C181199279 @default.
• W2086038644 hasConcept C190283241 @default.
• W2086038644 hasConcept C2775934118 @default.
• W2086038644 hasConcept C2777108182 @default.
• W2086038644 hasConcept C2777167505 @default.
• W2086038644 hasConcept C2777497464 @default.
• W2086038644 hasConcept C2778260397 @default.
• W2086038644 hasConcept C2780115381 @default.
• W2086038644 hasConcept C29537977 @default.
• W2086038644 hasConcept C31573885 @default.
• W2086038644 hasConcept C35195891 @default.
• W2086038644 hasConcept C54355233 @default.
• W2086038644 hasConcept C552990157 @default.
• W2086038644 hasConcept C553184892 @default.
• W2086038644 hasConcept C55493867 @default.
• W2086038644 hasConcept C62112901 @default.
• W2086038644 hasConcept C64241487 @default.
• W2086038644 hasConcept C73573662 @default.
• W2086038644 hasConcept C81885089 @default.
• W2086038644 hasConcept C86803240 @default.
• W2086038644 hasConcept C98086919 @default.
• W2086038644 hasConceptScore W2086038644C1491633281 @default.
• W2086038644 hasConceptScore W2086038644C153911025 @default.
• W2086038644 hasConceptScore W2086038644C175656101 @default.
• W2086038644 hasConceptScore W2086038644C181199279 @default.
• W2086038644 hasConceptScore W2086038644C190283241 @default.
• W2086038644 hasConceptScore W2086038644C2775934118 @default.
• W2086038644 hasConceptScore W2086038644C2777108182 @default.
• W2086038644 hasConceptScore W2086038644C2777167505 @default.
• W2086038644 hasConceptScore W2086038644C2777497464 @default.
• W2086038644 hasConceptScore W2086038644C2778260397 @default.
• W2086038644 hasConceptScore W2086038644C2780115381 @default.
• W2086038644 hasConceptScore W2086038644C29537977 @default.
• W2086038644 hasConceptScore W2086038644C31573885 @default.
• W2086038644 hasConceptScore W2086038644C35195891 @default.
• W2086038644 hasConceptScore W2086038644C54355233 @default.
• W2086038644 hasConceptScore W2086038644C552990157 @default.
• W2086038644 hasConceptScore W2086038644C553184892 @default.
• W2086038644 hasConceptScore W2086038644C55493867 @default.
• W2086038644 hasConceptScore W2086038644C62112901 @default.
• W2086038644 hasConceptScore W2086038644C64241487 @default.
• W2086038644 hasConceptScore W2086038644C73573662 @default.
• W2086038644 hasConceptScore W2086038644C81885089 @default.
• W2086038644 hasConceptScore W2086038644C86803240 @default.
• W2086038644 hasConceptScore W2086038644C98086919 @default.
• W2086038644 hasLocation W20860386441 @default.
• W2086038644 hasLocation W20860386442 @default.
• W2086038644 hasOpenAccess W2086038644 @default.
• W2086038644 hasPrimaryLocation W20860386441 @default.
• W2086038644 hasRelatedWork W1539637396 @default.
• W2086038644 hasRelatedWork W1943619266 @default.

• next