FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086075785> ?p ?o ?g. }

• W2086075785 endingPage "6781" @default.
• W2086075785 startingPage "6776" @default.
• W2086075785 abstract "Octyl beta-glucoside (1%), a dialyzable detergent, was used to solubilize the insulin receptor of the turkey erythrocyte membrane. Insulin binding capacity was stable for at least 1 week when the receptor was kept in 1% octyl beta-glucoside at 4 degrees C. The binding properties of the solubilized receptor were examined at detergent concentrations above (1%) and below (0.6%) the critical micelle concentration. A reduction in insulin binding occurred when the detergent concentration was raised above the critical micelle concentration, due to an apparent decrease in the number of binding sites. The specificity of the receptor for insulin analogues was preserved, and the relative affinity of the solubilized receptor, desoctapeptide insulin greater than proinsulin greater than porcine insulin, was similar in 0.6% and 1% detergent. Addition of divalent cations increased insulin binding to a similar extent at both detergent concentrations, but there was a slightly greater stimulation of binding in 0.6% detergent as compared to 1% detergent. The pH optimum for binding was not affected by changes in the detergent concentration. These results indicate that the insulin receptor can be successfully solubilized by octyl beta-glucoside and that the binding activity is quite stable. Therefore, octyl beta-glucoside may be a useful detergent for purification of this receptor. In addition, the data indicate that the binding properties of the insulin receptor can be affected by changes in the physical state of the octyl beta-glucoside." @default.
• W2086075785 created "2016-06-24" @default.
• W2086075785 creator A5001958946 @default.
• W2086075785 creator A5018129833 @default.
• W2086075785 creator A5071295235 @default.
• W2086075785 date "1981-11-01" @default.
• W2086075785 modified "2023-09-27" @default.
• W2086075785 title "Effects of octyl .beta.-glucoside on insulin binding to solubilized membrane receptors" @default.
• W2086075785 cites W14699222 @default.
• W2086075785 cites W1506549174 @default.
• W2086075785 cites W1535809564 @default.
• W2086075785 cites W1551972426 @default.
• W2086075785 cites W1573534810 @default.
• W2086075785 cites W1580451106 @default.
• W2086075785 cites W1581288183 @default.
• W2086075785 cites W1591826165 @default.
• W2086075785 cites W1599718697 @default.
• W2086075785 cites W175424067 @default.
• W2086075785 cites W1974898644 @default.
• W2086075785 cites W1976037530 @default.
• W2086075785 cites W1979800838 @default.
• W2086075785 cites W1982076963 @default.
• W2086075785 cites W1983041459 @default.
• W2086075785 cites W2006989237 @default.
• W2086075785 cites W2020513891 @default.
• W2086075785 cites W2029537609 @default.
• W2086075785 cites W2031018977 @default.
• W2086075785 cites W2042015440 @default.
• W2086075785 cites W2044398898 @default.
• W2086075785 cites W2046491869 @default.
• W2086075785 cites W2046760016 @default.
• W2086075785 cites W2047954857 @default.
• W2086075785 cites W2056477314 @default.
• W2086075785 cites W2074975964 @default.
• W2086075785 cites W2075649947 @default.
• W2086075785 cites W2078286981 @default.
• W2086075785 cites W2102131116 @default.
• W2086075785 cites W2122944464 @default.
• W2086075785 cites W2153435210 @default.
• W2086075785 cites W2196896858 @default.
• W2086075785 cites W2897677445 @default.
• W2086075785 doi "https://doi.org/10.1021/bi00527a006" @default.
• W2086075785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7032581" @default.
• W2086075785 hasPublicationYear "1981" @default.
• W2086075785 type Work @default.
• W2086075785 sameAs 2086075785 @default.
• W2086075785 citedByCount "62" @default.
• W2086075785 countsByYear W20860757852014 @default.
• W2086075785 crossrefType "journal-article" @default.
• W2086075785 hasAuthorship W2086075785A5001958946 @default.
• W2086075785 hasAuthorship W2086075785A5018129833 @default.
• W2086075785 hasAuthorship W2086075785A5071295235 @default.
• W2086075785 hasConcept C112446052 @default.
• W2086075785 hasConcept C11268172 @default.
• W2086075785 hasConcept C134018914 @default.
• W2086075785 hasConcept C142724271 @default.
• W2086075785 hasConcept C170493617 @default.
• W2086075785 hasConcept C178790620 @default.
• W2086075785 hasConcept C184651966 @default.
• W2086075785 hasConcept C185592680 @default.
• W2086075785 hasConcept C204787440 @default.
• W2086075785 hasConcept C2777391703 @default.
• W2086075785 hasConcept C2778987810 @default.
• W2086075785 hasConcept C2779306644 @default.
• W2086075785 hasConcept C2781347565 @default.
• W2086075785 hasConcept C41625074 @default.
• W2086075785 hasConcept C43617362 @default.
• W2086075785 hasConcept C4863142 @default.
• W2086075785 hasConcept C55493867 @default.
• W2086075785 hasConcept C64237528 @default.
• W2086075785 hasConcept C71924100 @default.
• W2086075785 hasConcept C74884574 @default.
• W2086075785 hasConcept C86803240 @default.
• W2086075785 hasConcept C90061646 @default.
• W2086075785 hasConceptScore W2086075785C112446052 @default.
• W2086075785 hasConceptScore W2086075785C11268172 @default.
• W2086075785 hasConceptScore W2086075785C134018914 @default.
• W2086075785 hasConceptScore W2086075785C142724271 @default.
• W2086075785 hasConceptScore W2086075785C170493617 @default.
• W2086075785 hasConceptScore W2086075785C178790620 @default.
• W2086075785 hasConceptScore W2086075785C184651966 @default.
• W2086075785 hasConceptScore W2086075785C185592680 @default.
• W2086075785 hasConceptScore W2086075785C204787440 @default.
• W2086075785 hasConceptScore W2086075785C2777391703 @default.
• W2086075785 hasConceptScore W2086075785C2778987810 @default.
• W2086075785 hasConceptScore W2086075785C2779306644 @default.
• W2086075785 hasConceptScore W2086075785C2781347565 @default.
• W2086075785 hasConceptScore W2086075785C41625074 @default.
• W2086075785 hasConceptScore W2086075785C43617362 @default.
• W2086075785 hasConceptScore W2086075785C4863142 @default.
• W2086075785 hasConceptScore W2086075785C55493867 @default.
• W2086075785 hasConceptScore W2086075785C64237528 @default.
• W2086075785 hasConceptScore W2086075785C71924100 @default.
• W2086075785 hasConceptScore W2086075785C74884574 @default.
• W2086075785 hasConceptScore W2086075785C86803240 @default.
• W2086075785 hasConceptScore W2086075785C90061646 @default.
• W2086075785 hasIssue "24" @default.
• W2086075785 hasLocation W20860757851 @default.

• next