Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086076082> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2086076082 abstract "A novel screening system using a microchamber array chip was developed for construction of combinatorial nano-sized protein libraries in combination with yeast cell surface engineering. It is possible to place a single yeast cell into each microchamber, to observe its behavior, and to pick up the target cell. The microchamber array chip is referred to as a “yeast cell chip.” A single EGFP-displaying yeast cell could be detected, picked up by a micro-manipulator, and cultivated on agar medium. Furthermore, a catalytic reaction, the hydrolysis of fluorescein dioctanate, by a single yeast cell displaying Rhizopus oryzae lipase (ROL) was carried out in one microchamber. The ROL-encoding gene in a single ROL-displaying cell was amplified by PCR. These results demonstrate that this yeast cell chip in combination with cell surface engineering could be used as a tool in a high-throughput screening system not only for a single living cell and a whole-cell catalyst with a nano-sized protein cluster but also for modified nano-sized and functional protein molecules from protein libraries on the cell surface." @default.
- W2086076082 created "2016-06-24" @default.
- W2086076082 creator A5021362767 @default.
- W2086076082 creator A5023244930 @default.
- W2086076082 creator A5029514198 @default.
- W2086076082 creator A5057737267 @default.
- W2086076082 creator A5063620609 @default.
- W2086076082 creator A5066931322 @default.
- W2086076082 creator A5072337493 @default.
- W2086076082 creator A5083312764 @default.
- W2086076082 creator A5087173459 @default.
- W2086076082 date "2005-01-01" @default.
- W2086076082 modified "2023-09-26" @default.
- W2086076082 title "Construction of Novel Single-Cell Screening System Using a Yeast Cell Chip for Nano-sized Modified-Protein-Displaying Libraries" @default.
- W2086076082 cites W1647597862 @default.
- W2086076082 cites W1964255752 @default.
- W2086076082 cites W1967053372 @default.
- W2086076082 cites W1970078207 @default.
- W2086076082 cites W1970588384 @default.
- W2086076082 cites W2021192048 @default.
- W2086076082 cites W2034160086 @default.
- W2086076082 cites W2042697202 @default.
- W2086076082 cites W2052302554 @default.
- W2086076082 cites W2059428836 @default.
- W2086076082 cites W2069649999 @default.
- W2086076082 cites W2070864278 @default.
- W2086076082 cites W2072183005 @default.
- W2086076082 cites W2080199782 @default.
- W2086076082 cites W2084150463 @default.
- W2086076082 cites W2094696727 @default.
- W2086076082 cites W2117138611 @default.
- W2086076082 cites W2127499426 @default.
- W2086076082 cites W2145367747 @default.
- W2086076082 cites W2157058776 @default.
- W2086076082 cites W2166435212 @default.
- W2086076082 cites W2171443261 @default.
- W2086076082 doi "https://doi.org/10.1385/nbt:1:1:105" @default.
- W2086076082 hasPublicationYear "2005" @default.
- W2086076082 type Work @default.
- W2086076082 sameAs 2086076082 @default.
- W2086076082 citedByCount "10" @default.
- W2086076082 countsByYear W20860760822015 @default.
- W2086076082 countsByYear W20860760822017 @default.
- W2086076082 countsByYear W20860760822019 @default.
- W2086076082 crossrefType "journal-article" @default.
- W2086076082 hasAuthorship W2086076082A5021362767 @default.
- W2086076082 hasAuthorship W2086076082A5023244930 @default.
- W2086076082 hasAuthorship W2086076082A5029514198 @default.
- W2086076082 hasAuthorship W2086076082A5057737267 @default.
- W2086076082 hasAuthorship W2086076082A5063620609 @default.
- W2086076082 hasAuthorship W2086076082A5066931322 @default.
- W2086076082 hasAuthorship W2086076082A5072337493 @default.
- W2086076082 hasAuthorship W2086076082A5083312764 @default.
- W2086076082 hasAuthorship W2086076082A5087173459 @default.
- W2086076082 hasConcept C100544194 @default.
- W2086076082 hasConcept C1491633281 @default.
- W2086076082 hasConcept C171250308 @default.
- W2086076082 hasConcept C185592680 @default.
- W2086076082 hasConcept C192562407 @default.
- W2086076082 hasConcept C2777576037 @default.
- W2086076082 hasConcept C2778541762 @default.
- W2086076082 hasConcept C2779222958 @default.
- W2086076082 hasConcept C55493867 @default.
- W2086076082 hasConcept C87892846 @default.
- W2086076082 hasConceptScore W2086076082C100544194 @default.
- W2086076082 hasConceptScore W2086076082C1491633281 @default.
- W2086076082 hasConceptScore W2086076082C171250308 @default.
- W2086076082 hasConceptScore W2086076082C185592680 @default.
- W2086076082 hasConceptScore W2086076082C192562407 @default.
- W2086076082 hasConceptScore W2086076082C2777576037 @default.
- W2086076082 hasConceptScore W2086076082C2778541762 @default.
- W2086076082 hasConceptScore W2086076082C2779222958 @default.
- W2086076082 hasConceptScore W2086076082C55493867 @default.
- W2086076082 hasConceptScore W2086076082C87892846 @default.
- W2086076082 hasLocation W20860760821 @default.
- W2086076082 hasOpenAccess W2086076082 @default.
- W2086076082 hasPrimaryLocation W20860760821 @default.
- W2086076082 hasRelatedWork W2365942097 @default.
- W2086076082 hasRelatedWork W2405674451 @default.
- W2086076082 hasRelatedWork W2481547772 @default.
- W2086076082 hasRelatedWork W2618790979 @default.
- W2086076082 hasRelatedWork W2748952813 @default.
- W2086076082 hasRelatedWork W2899084033 @default.
- W2086076082 hasRelatedWork W3041229667 @default.
- W2086076082 hasRelatedWork W3109893568 @default.
- W2086076082 hasRelatedWork W4240994900 @default.
- W2086076082 hasRelatedWork W4252804269 @default.
- W2086076082 isParatext "false" @default.
- W2086076082 isRetracted "false" @default.
- W2086076082 magId "2086076082" @default.
- W2086076082 workType "article" @default.