FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086095324> ?p ?o ?g. }

• W2086095324 endingPage "4804" @default.
• W2086095324 startingPage "4799" @default.
• W2086095324 abstract "Nattokinase, a serine protease, and pronattokinase, when expressed in Escherichia coli, formed insoluble aggregates without enzymatic activity. For functional expression and purification, nattokinase or pronattokinase was first overexpressed in E. coli as an insoluble recombinant protein linked to the C terminus of oleosin, a structural protein of seed oil bodies, by an intein fragment. Artificial oil bodies were reconstituted with triacylglycerol, phospholipid, and the insoluble recombinant protein thus formed. Soluble nattokinase was subsequently released through self-splicing of intein induced by temperature alteration, with the remaining oleosin−intein residing in oil bodies and the leading propeptide of pronattokinase, when present, spontaneously cleaved in the process. Active nattokinase with fibrinolytic activity was harvested by concentrating the supernatant. Nattokinase released from oleosin−intein−pronattokinase exhibited 5 times higher activity than that released from oleosin−intein−nattokinase, although the production yields were similar in both cases. Furthermore, active nattokinase could be harvested in the same system by fusing pronattokinase to the N terminus of oleosin via a different intein linker, with self-splicing induced by 1,4-dithiothreitol. These results have shown a great potential of this system for bacterial expression and purification of functional recombinant proteins. Keywords: Artificial oil body; intein; nattokinase; oleosin; propeptide" @default.
• W2086095324 created "2016-06-24" @default.
• W2086095324 creator A5044839154 @default.
• W2086095324 creator A5045736266 @default.
• W2086095324 creator A5049958886 @default.
• W2086095324 creator A5064101037 @default.
• W2086095324 date "2005-05-03" @default.
• W2086095324 modified "2023-10-16" @default.
• W2086095324 title "Efficient System of Artificial Oil Bodies for Functional Expression and Purification of Recombinant Nattokinase in <i>Escherichia coli</i>" @default.
• W2086095324 cites W1513208225 @default.
• W2086095324 cites W1591438776 @default.
• W2086095324 cites W1612480077 @default.
• W2086095324 cites W1967149839 @default.
• W2086095324 cites W1974185738 @default.
• W2086095324 cites W2068450796 @default.
• W2086095324 cites W2083532852 @default.
• W2086095324 cites W2092603929 @default.
• W2086095324 cites W2114522205 @default.
• W2086095324 cites W2161904803 @default.
• W2086095324 cites W2162015038 @default.
• W2086095324 doi "https://doi.org/10.1021/jf050264a" @default.
• W2086095324 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15941319" @default.
• W2086095324 hasPublicationYear "2005" @default.
• W2086095324 type Work @default.
• W2086095324 sameAs 2086095324 @default.
• W2086095324 citedByCount "66" @default.
• W2086095324 countsByYear W20860953242012 @default.
• W2086095324 countsByYear W20860953242013 @default.
• W2086095324 countsByYear W20860953242014 @default.
• W2086095324 countsByYear W20860953242015 @default.
• W2086095324 countsByYear W20860953242016 @default.
• W2086095324 countsByYear W20860953242017 @default.
• W2086095324 countsByYear W20860953242018 @default.
• W2086095324 countsByYear W20860953242019 @default.
• W2086095324 countsByYear W20860953242021 @default.
• W2086095324 countsByYear W20860953242022 @default.
• W2086095324 countsByYear W20860953242023 @default.
• W2086095324 crossrefType "journal-article" @default.
• W2086095324 hasAuthorship W2086095324A5044839154 @default.
• W2086095324 hasAuthorship W2086095324A5045736266 @default.
• W2086095324 hasAuthorship W2086095324A5049958886 @default.
• W2086095324 hasAuthorship W2086095324A5064101037 @default.
• W2086095324 hasConcept C100544194 @default.
• W2086095324 hasConcept C104317684 @default.
• W2086095324 hasConcept C123894998 @default.
• W2086095324 hasConcept C145508813 @default.
• W2086095324 hasConcept C181199279 @default.
• W2086095324 hasConcept C182220744 @default.
• W2086095324 hasConcept C185592680 @default.
• W2086095324 hasConcept C190283241 @default.
• W2086095324 hasConcept C193469717 @default.
• W2086095324 hasConcept C203522944 @default.
• W2086095324 hasConcept C203750385 @default.
• W2086095324 hasConcept C2775954774 @default.
• W2086095324 hasConcept C2778568395 @default.
• W2086095324 hasConcept C40767141 @default.
• W2086095324 hasConcept C54458228 @default.
• W2086095324 hasConcept C547475151 @default.
• W2086095324 hasConcept C55493867 @default.
• W2086095324 hasConcept C60732484 @default.
• W2086095324 hasConcept C67705224 @default.
• W2086095324 hasConcept C86803240 @default.
• W2086095324 hasConceptScore W2086095324C100544194 @default.
• W2086095324 hasConceptScore W2086095324C104317684 @default.
• W2086095324 hasConceptScore W2086095324C123894998 @default.
• W2086095324 hasConceptScore W2086095324C145508813 @default.
• W2086095324 hasConceptScore W2086095324C181199279 @default.
• W2086095324 hasConceptScore W2086095324C182220744 @default.
• W2086095324 hasConceptScore W2086095324C185592680 @default.
• W2086095324 hasConceptScore W2086095324C190283241 @default.
• W2086095324 hasConceptScore W2086095324C193469717 @default.
• W2086095324 hasConceptScore W2086095324C203522944 @default.
• W2086095324 hasConceptScore W2086095324C203750385 @default.
• W2086095324 hasConceptScore W2086095324C2775954774 @default.
• W2086095324 hasConceptScore W2086095324C2778568395 @default.
• W2086095324 hasConceptScore W2086095324C40767141 @default.
• W2086095324 hasConceptScore W2086095324C54458228 @default.
• W2086095324 hasConceptScore W2086095324C547475151 @default.
• W2086095324 hasConceptScore W2086095324C55493867 @default.
• W2086095324 hasConceptScore W2086095324C60732484 @default.
• W2086095324 hasConceptScore W2086095324C67705224 @default.
• W2086095324 hasConceptScore W2086095324C86803240 @default.
• W2086095324 hasIssue "12" @default.
• W2086095324 hasLocation W20860953241 @default.
• W2086095324 hasLocation W20860953242 @default.
• W2086095324 hasOpenAccess W2086095324 @default.
• W2086095324 hasPrimaryLocation W20860953241 @default.
• W2086095324 hasRelatedWork W1967665958 @default.
• W2086095324 hasRelatedWork W2007930265 @default.
• W2086095324 hasRelatedWork W2019922031 @default.
• W2086095324 hasRelatedWork W2086095324 @default.
• W2086095324 hasRelatedWork W2092993483 @default.
• W2086095324 hasRelatedWork W2155425593 @default.
• W2086095324 hasRelatedWork W2238024296 @default.
• W2086095324 hasRelatedWork W2382787658 @default.
• W2086095324 hasRelatedWork W31324123 @default.
• W2086095324 hasRelatedWork W3144801213 @default.
• W2086095324 hasVolume "53" @default.

• next