FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086102168> ?p ?o ?g. }

• W2086102168 endingPage "558" @default.
• W2086102168 startingPage "549" @default.
• W2086102168 abstract "The Escherichia coli Lac and Gal repressors are two members of a large family of bacterial repressor proteins that share significant sequence and structural homology. Efficient repression by all family members requires specific binding to a site or sites close to the transcriptional start of the genes regulated. Both LacR and GalR have to bind to at least two sites for efficient repression, yet they differ in one important respect: LacR is a homotetramer whereas GalR is a homodimer. In an attempt to understand this difference, we studied the operator binding activity of a LacR variant that has the DNA-binding specificity of GalR (LacR-V17A18). A tetrameric version of this protein shows a 30-fold decrease in association rate to operator located on a long (λ) DNA molecule, in comparison to wild-type LacR, while a dimeric version of this protein shows an unaltered association rate in comparison to dimeric LacR. This reduction in association rate correlates with a broadened DNA-binding specificity for base-pairs 4 and 5 of the operator: examination of an additional LacR variant with an even broader DNA-binding specificity indicates that a tetrameric version also shows a 30-fold decrease in association rate in comparison to wild-type LacR, while a dimeric version again shows an unaltered association rate in comparison to dimeric LacR. This difference in association rate in vitro correlates with whether a tetrameric or dimeric variant of LacR of a given DNA-binding specificity will repress lacZ under control of a single operator more efficiently in vivo. We therefore propose that the formation of stable homotetramers becomes a distinct disadvantage unless a high degree of DNA-binding specificity is also present, and demonstrate that this in indeed the case for GalR-mediated repression of the gal operon. This functional constraint seems to have influenced the evolution of the LacI-GalR family of repressors, most of which have a relatively broad specificity of DNA-binding and most of which form only stable homodimers." @default.
• W2086102168 created "2016-06-24" @default.
• W2086102168 creator A5000339271 @default.
• W2086102168 creator A5060850966 @default.
• W2086102168 creator A5064572287 @default.
• W2086102168 creator A5082957193 @default.
• W2086102168 date "1998-05-01" @default.
• W2086102168 modified "2023-10-18" @default.
• W2086102168 title "Operator search by mutant lac repressors" @default.
• W2086102168 cites W107862656 @default.
• W2086102168 cites W133821640 @default.
• W2086102168 cites W1497340069 @default.
• W2086102168 cites W1499816395 @default.
• W2086102168 cites W1510818199 @default.
• W2086102168 cites W1518153536 @default.
• W2086102168 cites W1548869612 @default.
• W2086102168 cites W1573008071 @default.
• W2086102168 cites W1576465448 @default.
• W2086102168 cites W1589832944 @default.
• W2086102168 cites W1591758727 @default.
• W2086102168 cites W1603900493 @default.
• W2086102168 cites W181956194 @default.
• W2086102168 cites W1930951784 @default.
• W2086102168 cites W1974450146 @default.
• W2086102168 cites W1980946235 @default.
• W2086102168 cites W1981333260 @default.
• W2086102168 cites W1985247203 @default.
• W2086102168 cites W1987063642 @default.
• W2086102168 cites W1988103323 @default.
• W2086102168 cites W1990507623 @default.
• W2086102168 cites W1995514232 @default.
• W2086102168 cites W1998105880 @default.
• W2086102168 cites W1999912383 @default.
• W2086102168 cites W2005230697 @default.
• W2086102168 cites W2009217296 @default.
• W2086102168 cites W2014038010 @default.
• W2086102168 cites W2014201888 @default.
• W2086102168 cites W2019196439 @default.
• W2086102168 cites W2032619610 @default.
• W2086102168 cites W2033529844 @default.
• W2086102168 cites W2039202354 @default.
• W2086102168 cites W2052131140 @default.
• W2086102168 cites W2052487654 @default.
• W2086102168 cites W2063237027 @default.
• W2086102168 cites W2066267034 @default.
• W2086102168 cites W2066825888 @default.
• W2086102168 cites W2067165394 @default.
• W2086102168 cites W2072920810 @default.
• W2086102168 cites W2074621528 @default.
• W2086102168 cites W2075147973 @default.
• W2086102168 cites W2077539492 @default.
• W2086102168 cites W2079405355 @default.
• W2086102168 cites W2080599534 @default.
• W2086102168 cites W2084916160 @default.
• W2086102168 cites W2085719587 @default.
• W2086102168 cites W2091295803 @default.
• W2086102168 cites W2093668818 @default.
• W2086102168 cites W2112335620 @default.
• W2086102168 cites W2133274430 @default.
• W2086102168 cites W2152842787 @default.
• W2086102168 cites W2159949023 @default.
• W2086102168 cites W2161653032 @default.
• W2086102168 cites W2170207807 @default.
• W2086102168 cites W2199478369 @default.
• W2086102168 cites W456191 @default.
• W2086102168 cites W58950924 @default.
• W2086102168 doi "https://doi.org/10.1006/jmbi.1998.1729" @default.
• W2086102168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9600838" @default.
• W2086102168 hasPublicationYear "1998" @default.
• W2086102168 type Work @default.
• W2086102168 sameAs 2086102168 @default.
• W2086102168 citedByCount "24" @default.
• W2086102168 countsByYear W20861021682016 @default.
• W2086102168 countsByYear W20861021682018 @default.
• W2086102168 countsByYear W20861021682021 @default.
• W2086102168 crossrefType "journal-article" @default.
• W2086102168 hasAuthorship W2086102168A5000339271 @default.
• W2086102168 hasAuthorship W2086102168A5060850966 @default.
• W2086102168 hasAuthorship W2086102168A5064572287 @default.
• W2086102168 hasAuthorship W2086102168A5082957193 @default.
• W2086102168 hasConcept C104317684 @default.
• W2086102168 hasConcept C113629113 @default.
• W2086102168 hasConcept C143065580 @default.
• W2086102168 hasConcept C150194340 @default.
• W2086102168 hasConcept C158448853 @default.
• W2086102168 hasConcept C17020691 @default.
• W2086102168 hasConcept C170344550 @default.
• W2086102168 hasConcept C185592680 @default.
• W2086102168 hasConcept C186310378 @default.
• W2086102168 hasConcept C22744801 @default.
• W2086102168 hasConcept C54355233 @default.
• W2086102168 hasConcept C552990157 @default.
• W2086102168 hasConcept C71240020 @default.
• W2086102168 hasConcept C86339819 @default.
• W2086102168 hasConcept C86803240 @default.
• W2086102168 hasConceptScore W2086102168C104317684 @default.
• W2086102168 hasConceptScore W2086102168C113629113 @default.
• W2086102168 hasConceptScore W2086102168C143065580 @default.

• next