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- W2086171773 abstract "Abstract The postmitochondrial pellet from SFV-infected cells was incubated under in vitro RNA-synthesizing conditions in the presence of [ 3 H]UTP to label nascent chains in the RNA replication complex. The solubilized complex was purified by centrifugation in a glycerol gradient followed by a sucrose gradient in which it sedimented with a peak at 34 S. It contained double-stranded RNA which by mild ribonuclease treatment was converted to replicative forms I, II, and III, indicating that it was derived from replicative intermediates synthesizing 42 S and 26 S RNA. A similar structure was also isolated from infected cells labeled with [ 3 H]uridine early in infection. The only virus-specific proteins associated with the complex were ns70, ns72, and ns86. The presence of ns70 and ns72 was confirmed by tryptic peptide mapping." @default.
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- W2086171773 date "1979-10-01" @default.
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- W2086171773 title "Solubilized RNA replication complex from Semliki Forest virus-infected cells" @default.
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- W2086171773 doi "https://doi.org/10.1016/0042-6822(79)90553-1" @default.
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