Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086172313> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2086172313 endingPage "6554" @default.
- W2086172313 startingPage "6546" @default.
- W2086172313 abstract "The proximal bond between the iron atom of the heme group and the N epsilon of histidine F8 in myoglobin (Mb) and hemoglobin (Hb) is presumed to be an important determinant of heme binding, protein structure, and oxygen binding. Here a system is described in which the proximal ligand is provided intermolecularly by the histidine side chain mimic imidazole. The proximal ligand of sperm whale Mb is replaced with glycine (H93G) using site-directed mutagenesis. The addition of imidazole to Escherichia coli expressing this gene reconstitutes myoglobin function. H93G Mb purified in the presence of imidazole is spectroscopically similar to wild-type Mb in combination with a wide variety of distal ligands. The crystal structure of H93G Mb, determined in the presence of imidazole, reveals that an imidazole molecule is bonded to the heme iron on the proximal side, substituting in trans for the side-chain function of the proximal histidine of wild-type Mb. Although H93G Mb is similar in spectroscopic and gross structural detail to wild-type Mb, subtle differences exist in the orientation of imidazole with respect to the heme group. trans-Complementation of proximal ligand function will allow the proximal bond in hemoproteins to be chemically substituted beyond the limits of the genetic code." @default.
- W2086172313 created "2016-06-24" @default.
- W2086172313 creator A5019100333 @default.
- W2086172313 date "1994-05-01" @default.
- W2086172313 modified "2023-10-12" @default.
- W2086172313 title "Replacement of the Proximal Ligand of Sperm Whale Myoglobin with Free Imidazole in the Mutant His-93.fwdarw.Gly" @default.
- W2086172313 cites W1512775211 @default.
- W2086172313 cites W1972433518 @default.
- W2086172313 cites W1973724691 @default.
- W2086172313 cites W1998808290 @default.
- W2086172313 cites W2012057187 @default.
- W2086172313 cites W2026182346 @default.
- W2086172313 cites W2026911129 @default.
- W2086172313 cites W2053947406 @default.
- W2086172313 cites W2055847384 @default.
- W2086172313 cites W2057984729 @default.
- W2086172313 cites W2061288875 @default.
- W2086172313 cites W2068555097 @default.
- W2086172313 cites W2069670931 @default.
- W2086172313 cites W2073198049 @default.
- W2086172313 cites W2075284376 @default.
- W2086172313 cites W2084459566 @default.
- W2086172313 cites W2086044842 @default.
- W2086172313 cites W2088347357 @default.
- W2086172313 cites W2095873332 @default.
- W2086172313 cites W2100837269 @default.
- W2086172313 cites W2131643591 @default.
- W2086172313 cites W2140040671 @default.
- W2086172313 cites W2155941123 @default.
- W2086172313 cites W24063391 @default.
- W2086172313 cites W2438131745 @default.
- W2086172313 cites W2499926109 @default.
- W2086172313 cites W3028068522 @default.
- W2086172313 doi "https://doi.org/10.1021/bi00187a023" @default.
- W2086172313 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8204590" @default.
- W2086172313 hasPublicationYear "1994" @default.
- W2086172313 type Work @default.
- W2086172313 sameAs 2086172313 @default.
- W2086172313 citedByCount "140" @default.
- W2086172313 countsByYear W20861723132012 @default.
- W2086172313 countsByYear W20861723132013 @default.
- W2086172313 countsByYear W20861723132014 @default.
- W2086172313 countsByYear W20861723132015 @default.
- W2086172313 countsByYear W20861723132016 @default.
- W2086172313 countsByYear W20861723132017 @default.
- W2086172313 countsByYear W20861723132018 @default.
- W2086172313 countsByYear W20861723132020 @default.
- W2086172313 countsByYear W20861723132021 @default.
- W2086172313 countsByYear W20861723132022 @default.
- W2086172313 crossrefType "journal-article" @default.
- W2086172313 hasAuthorship W2086172313A5019100333 @default.
- W2086172313 hasConcept C116569031 @default.
- W2086172313 hasConcept C170493617 @default.
- W2086172313 hasConcept C181199279 @default.
- W2086172313 hasConcept C185592680 @default.
- W2086172313 hasConcept C20705724 @default.
- W2086172313 hasConcept C2776217839 @default.
- W2086172313 hasConcept C2778063876 @default.
- W2086172313 hasConcept C2778460671 @default.
- W2086172313 hasConcept C2780874372 @default.
- W2086172313 hasConcept C515207424 @default.
- W2086172313 hasConcept C55493867 @default.
- W2086172313 hasConcept C71240020 @default.
- W2086172313 hasConcept C8010536 @default.
- W2086172313 hasConceptScore W2086172313C116569031 @default.
- W2086172313 hasConceptScore W2086172313C170493617 @default.
- W2086172313 hasConceptScore W2086172313C181199279 @default.
- W2086172313 hasConceptScore W2086172313C185592680 @default.
- W2086172313 hasConceptScore W2086172313C20705724 @default.
- W2086172313 hasConceptScore W2086172313C2776217839 @default.
- W2086172313 hasConceptScore W2086172313C2778063876 @default.
- W2086172313 hasConceptScore W2086172313C2778460671 @default.
- W2086172313 hasConceptScore W2086172313C2780874372 @default.
- W2086172313 hasConceptScore W2086172313C515207424 @default.
- W2086172313 hasConceptScore W2086172313C55493867 @default.
- W2086172313 hasConceptScore W2086172313C71240020 @default.
- W2086172313 hasConceptScore W2086172313C8010536 @default.
- W2086172313 hasIssue "21" @default.
- W2086172313 hasLocation W20861723131 @default.
- W2086172313 hasLocation W20861723132 @default.
- W2086172313 hasOpenAccess W2086172313 @default.
- W2086172313 hasPrimaryLocation W20861723131 @default.
- W2086172313 hasRelatedWork W1965021988 @default.
- W2086172313 hasRelatedWork W1976141240 @default.
- W2086172313 hasRelatedWork W1993624568 @default.
- W2086172313 hasRelatedWork W2016308157 @default.
- W2086172313 hasRelatedWork W2024174916 @default.
- W2086172313 hasRelatedWork W2038038833 @default.
- W2086172313 hasRelatedWork W2053379218 @default.
- W2086172313 hasRelatedWork W2068379918 @default.
- W2086172313 hasRelatedWork W2086172313 @default.
- W2086172313 hasRelatedWork W2165292961 @default.
- W2086172313 hasVolume "33" @default.
- W2086172313 isParatext "false" @default.
- W2086172313 isRetracted "false" @default.
- W2086172313 magId "2086172313" @default.
- W2086172313 workType "article" @default.