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- W2086256870 abstract "Pressure homogenization and subcellular fractionation has been performed on washed, human platelets and platelets treated with thrombin to undergo the so-called release reaction. Electron microscopy revealed that the particulate zones obtained from the control sample corresponded to membrane visicles (B), small storage granules (D) as well as mitochondria and larger storage granules (E). Only a few storage granules could be observed in the particulate zones isolated from thrombin-treated platelets. Visual comparison of the sucrose gradient patterns revealed that one granule fraction (D) had dissappeared from the thrombin-treated sampl. Sodium dodecyclysulfate-polyacrylamide gel electrophoresis showed a major protein band (mol. wt 145 500 ± 1000) in the extracellular phase (supernatant after removal of the platelets) of the thrombin-treated sample and in the granule fractions (D and E) of the control (mol. wt 147 000 ± 1000). Incubation of whole, washed platelets with thrombin for 5 min 37°C followed by sodium dodecylsulfate-polyacrylamide gel electrophoresis of the isolated membrane fraction revealed no reporducible differences in the protein band pattern compared to menbrane isolated from control platelets. However, after treatment with thrombin for 30 min, a protein band (mol. wt 183 000 ± 3500 had disappeared. The distribution of protein and β-N-acetylgluciosaminidase activity among the subcellular fractions were measured. Both were mainly recovered in the soluble fraction (>77%). The granule fractions, D and E of the control contained 30% ± 0.8% and 6.4% ± 1.3% of the total amount of β-N-acetylglucosaminidase in the gradient. Fraction E of the thormbin-treated cells contained 3.3% ± 1.0% of total while fraction D was lacking." @default.
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- W2086256870 date "1975-06-01" @default.
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- W2086256870 title "Effects of thrombin on washed, human platelets: Chnages in the subcellular fractions" @default.
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- W2086256870 doi "https://doi.org/10.1016/0304-4165(75)90006-9" @default.
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