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- W2086290527 abstract "Nanopore sequencing uses an ion current through a pore to identify nucleotides of single stranded DNA also driven through the pore. We previously demonstrated a technique where multiple double stranded sections are inserted in single stranded DNA. These double stranded sections halt the progression of the DNA, and provide enough time to use the current to identify the nucleotides held in the pore's constriction. When the force on the DNA dissociates a given duplex, the DNA moves to the next duplex section allowing a sequential read of the DNA. This sequencing method, termed duplex interrupt (DI) sequencing, does not easily detect repeated-nucleotides as they have the same current signature. Here we present a method to produce a distinct current level when the DNA progresses allowing repeat nucleotides to be determined." @default.
- W2086290527 created "2016-06-24" @default.
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- W2086290527 date "2012-01-01" @default.
- W2086290527 modified "2023-09-30" @default.
- W2086290527 title "Repeated Nucleotide Detection with Duplex Interrupted Nanopore Sequencing in MspA" @default.
- W2086290527 doi "https://doi.org/10.1016/j.bpj.2011.11.3953" @default.
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