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• W2086295440 startingPage "175" @default.
• W2086295440 abstract "Abstract A cell line, WE ‐cfin11e, with an epithelial‐like morphology was developed from a caudal fin of walleye, S ander vitreus ( M itchill), characterized as distinct from the established walleye caudal fin fibroblast‐like cell line, WE ‐cfin11f, and compared with WE ‐cfin11f for susceptibility to VHSV IV b. Immunocytochemistry and confocal microscopy were used to localize the intermediate filament protein, vimentin, the tight junction protein, zonula occludens‐1 ( ZO ‐1), the extracellular matrix protein, collagen I , and the viral protein, G . Although both cell lines contained vimentin, only WE ‐cfin11e stained for ZO ‐1 and only WE ‐cfin11f stained for collagen I . Ascorbic acid increased the accumulation of collagen I and caused the appearance of collagen fibres only in WE ‐cfin11f cultures. At 14 °C, both cell lines produced VHSV IV b, but the infection developed more rapidly in WE ‐cfin11f. At 4 °C, both cell lines became infected with VHSV IV b as judged by the expression of viral proteins, N and G , but only WE ‐cfin11f produced virus. The results suggest that cold temperatures can modulate viral tropism." @default.
• W2086295440 created "2016-06-24" @default.
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• W2086295440 date "2015-01-29" @default.
• W2086295440 modified "2023-10-03" @default.
• W2086295440 title "Differential viral haemorrhagic septicaemia virus genotype IVb infection in fin fibroblast and epithelial cell lines from walleye, <i>Sander vitreus</i> (Mitchill), at cold temperatures" @default.
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• W2086295440 doi "https://doi.org/10.1111/jfd.12345" @default.
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