FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086307622> ?p ?o ?g. }

• W2086307622 endingPage "492" @default.
• W2086307622 startingPage "488" @default.
• W2086307622 abstract "We have examined the voltage-dependent gating of batrachotoxin-modified sodium channels purified from rabbit T-tubular membranes in two ways. First, purified channels were reconstituted into planar bilayers and single-channel properties were measured. Batrachotoxin-activated channels showed steep voltage-dependent activation with half-maximal opening probabilities at potentials between -95 and -116 mV. The single-channel conductance (500 mM Na+ cis, 200 mM Na+ trans) averaged 20 pS and was independent of membrane potential. Channels usually inserted with their extracellular faces on the trans side of the bilayer; addition of tetrodotoxin to the cis side had no effect, whereas addition to the trans side blocked greater than 95% of channel openings at -77 mV. A second approach was used to establish that this voltage dependence was a characteristic of the entire population of purified channels and not just those few channels observed in planar bilayers. Channels reconstituted into egg phosphatidylcholine vesicles were functionally oriented by inclusion of internal saxitoxin; vesicle membrane potentials were then generated by K+ gradients in the presence of valinomycin. After batrachotoxin activation, Vm was altered by shifts of K+o. All of the specific 22Na+ influx activated by batrachotoxin and blocked by saxitoxin was found to be voltage sensitive, activating between predicted membrane potentials of -100 and -50 mV. The single-channel properties of the purified T-tubular sodium channel correspond closely to those seen with native sodium channels from rat sarcolemma. The voltage-dependent activation of the batrachotoxin-modified reconstituted channel is the same as that seen with native channels in situ or in bilayers after exposure to this toxin. Most importantly, this voltage-dependent gating is a property of all of the purified channels capable of specific pharmacological activation." @default.
• W2086307622 created "2016-06-24" @default.
• W2086307622 creator A5053686604 @default.
• W2086307622 creator A5063861589 @default.
• W2086307622 creator A5074371611 @default.
• W2086307622 creator A5087249928 @default.
• W2086307622 date "1986-01-01" @default.
• W2086307622 modified "2023-09-27" @default.
• W2086307622 title "Voltage-dependent activation in purified reconstituted sodium channels from rabbit T-tubular membranes." @default.
• W2086307622 cites W133556906 @default.
• W2086307622 cites W1497344470 @default.
• W2086307622 cites W1550583938 @default.
• W2086307622 cites W1553693448 @default.
• W2086307622 cites W1653329430 @default.
• W2086307622 cites W1967166972 @default.
• W2086307622 cites W1971419283 @default.
• W2086307622 cites W1986291491 @default.
• W2086307622 cites W1988727405 @default.
• W2086307622 cites W1995787167 @default.
• W2086307622 cites W2001872834 @default.
• W2086307622 cites W2003886214 @default.
• W2086307622 cites W2014071501 @default.
• W2086307622 cites W2018155809 @default.
• W2086307622 cites W2036770754 @default.
• W2086307622 cites W2045754403 @default.
• W2086307622 cites W2052510892 @default.
• W2086307622 cites W2060089222 @default.
• W2086307622 cites W2077576114 @default.
• W2086307622 cites W2078700589 @default.
• W2086307622 cites W2079425635 @default.
• W2086307622 cites W2087383295 @default.
• W2086307622 cites W2092637977 @default.
• W2086307622 cites W2097186716 @default.
• W2086307622 cites W2105979237 @default.
• W2086307622 cites W2113584220 @default.
• W2086307622 cites W2155799752 @default.
• W2086307622 doi "https://doi.org/10.1073/pnas.83.2.488" @default.
• W2086307622 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/322885" @default.
• W2086307622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2417245" @default.
• W2086307622 hasPublicationYear "1986" @default.
• W2086307622 type Work @default.
• W2086307622 sameAs 2086307622 @default.
• W2086307622 citedByCount "29" @default.
• W2086307622 crossrefType "journal-article" @default.
• W2086307622 hasAuthorship W2086307622A5053686604 @default.
• W2086307622 hasAuthorship W2086307622A5063861589 @default.
• W2086307622 hasAuthorship W2086307622A5074371611 @default.
• W2086307622 hasAuthorship W2086307622A5087249928 @default.
• W2086307622 hasBestOaLocation W20863076221 @default.
• W2086307622 hasConcept C114614502 @default.
• W2086307622 hasConcept C121932024 @default.
• W2086307622 hasConcept C123419017 @default.
• W2086307622 hasConcept C12554922 @default.
• W2086307622 hasConcept C130316041 @default.
• W2086307622 hasConcept C144024400 @default.
• W2086307622 hasConcept C149923435 @default.
• W2086307622 hasConcept C158685282 @default.
• W2086307622 hasConcept C170493617 @default.
• W2086307622 hasConcept C178790620 @default.
• W2086307622 hasConcept C181911157 @default.
• W2086307622 hasConcept C185592680 @default.
• W2086307622 hasConcept C194544171 @default.
• W2086307622 hasConcept C2780456920 @default.
• W2086307622 hasConcept C2908647359 @default.
• W2086307622 hasConcept C33923547 @default.
• W2086307622 hasConcept C39944091 @default.
• W2086307622 hasConcept C41625074 @default.
• W2086307622 hasConcept C50254741 @default.
• W2086307622 hasConcept C50952357 @default.
• W2086307622 hasConcept C537181965 @default.
• W2086307622 hasConcept C55493867 @default.
• W2086307622 hasConcept C80161118 @default.
• W2086307622 hasConcept C86803240 @default.
• W2086307622 hasConceptScore W2086307622C114614502 @default.
• W2086307622 hasConceptScore W2086307622C121932024 @default.
• W2086307622 hasConceptScore W2086307622C123419017 @default.
• W2086307622 hasConceptScore W2086307622C12554922 @default.
• W2086307622 hasConceptScore W2086307622C130316041 @default.
• W2086307622 hasConceptScore W2086307622C144024400 @default.
• W2086307622 hasConceptScore W2086307622C149923435 @default.
• W2086307622 hasConceptScore W2086307622C158685282 @default.
• W2086307622 hasConceptScore W2086307622C170493617 @default.
• W2086307622 hasConceptScore W2086307622C178790620 @default.
• W2086307622 hasConceptScore W2086307622C181911157 @default.
• W2086307622 hasConceptScore W2086307622C185592680 @default.
• W2086307622 hasConceptScore W2086307622C194544171 @default.
• W2086307622 hasConceptScore W2086307622C2780456920 @default.
• W2086307622 hasConceptScore W2086307622C2908647359 @default.
• W2086307622 hasConceptScore W2086307622C33923547 @default.
• W2086307622 hasConceptScore W2086307622C39944091 @default.
• W2086307622 hasConceptScore W2086307622C41625074 @default.
• W2086307622 hasConceptScore W2086307622C50254741 @default.
• W2086307622 hasConceptScore W2086307622C50952357 @default.
• W2086307622 hasConceptScore W2086307622C537181965 @default.
• W2086307622 hasConceptScore W2086307622C55493867 @default.
• W2086307622 hasConceptScore W2086307622C80161118 @default.
• W2086307622 hasConceptScore W2086307622C86803240 @default.
• W2086307622 hasIssue "2" @default.

• next