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- W2086415219 abstract "A rapid method is described for the detection of heterogeneity in a bacterial population with respect to a membrane marker. The technique involves treatment with penicillin in the presence of a carbon source, the utilization of which is limited by the inducible membrane marker, usually a permease. The part of the population containing the marker is lysed rapidly, and the cells devoid of the marker can be separated. The distribution of Lac-permease and Mel-permease in the progeny of fully induced populations during growth and cell division in the absence of inducer was examined using this technique. A non-synchronous population gives rise to approximately 50% of permease-less segregants after three generation times in mineral medium with glycerol or glucose as sole carbon source. These results can be interpreted in terms of a simple model of membrane growth." @default.
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- W2086415219 date "1971-12-01" @default.
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- W2086415219 title "Segregation of membrane markers during cell division in Escherichia coli. II. Segregation of Lac-permease and Mel-permease studied with a penicillin technique" @default.
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- W2086415219 doi "https://doi.org/10.1016/0005-2736(71)90141-6" @default.
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