Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086458510> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2086458510 abstract "T-A cloning takes advantage of the unpaired adenosyl residue added to the 3′ terminus of amplified DNAs by Taq and other thermostable DNA polymerase and uses a linearized plasmid vector with a protruding 3′ thymidylate residue at each of its 3′ termini to clone polymerase chain reaction (PCR)-derived DNA fragments. It is a simple, reliable, and efficient ligation-dependent cloning method for PCR products, but the drawback of variable cloning efficiency occurs during application. In the present work, the relationship between variable T-A cloning efficiency and the different 5′ end nucleotide base of primers used in PCR amplification was studied. The results showed that different cloning efficiency was obtained with different primer pairs containing A, T, C and G at the 5′ terminus respectively. The data shows that when the 5′ end base of primer pair was adenosyl, more white colonies could be obtained in cloning the corresponding PCR product in comparison with other bases. And the least white colonies were formed when using the primer pair with 5′ cytidylate end. The gluanylate end primers resulted in almost the same cloning efficiency in the white colonies amount as the thymidylate end primer did, and this efficiency was much lower than that of adenosyl end primers. This presumably is a consequence of variability in 3′dA addition to PCR products mediated by Taq polymerase. Our results offer instructions for primer design for researchers who choose T-A cloning to clone PCR products." @default.
- W2086458510 created "2016-06-24" @default.
- W2086458510 creator A5000386208 @default.
- W2086458510 creator A5033808402 @default.
- W2086458510 creator A5036443954 @default.
- W2086458510 creator A5063209584 @default.
- W2086458510 date "2007-03-01" @default.
- W2086458510 modified "2023-09-24" @default.
- W2086458510 title "Changes of 5' Terminal Nucleotides of PCR Primers Causing Variable T-A Cloning Efficiency" @default.
- W2086458510 cites W1988765112 @default.
- W2086458510 cites W1988983348 @default.
- W2086458510 cites W2021236910 @default.
- W2086458510 doi "https://doi.org/10.1111/j.1744-7909.2007.00430.x" @default.
- W2086458510 hasPublicationYear "2007" @default.
- W2086458510 type Work @default.
- W2086458510 sameAs 2086458510 @default.
- W2086458510 citedByCount "0" @default.
- W2086458510 crossrefType "journal-article" @default.
- W2086458510 hasAuthorship W2086458510A5000386208 @default.
- W2086458510 hasAuthorship W2086458510A5033808402 @default.
- W2086458510 hasAuthorship W2086458510A5036443954 @default.
- W2086458510 hasAuthorship W2086458510A5063209584 @default.
- W2086458510 hasConcept C104317684 @default.
- W2086458510 hasConcept C112456955 @default.
- W2086458510 hasConcept C121050878 @default.
- W2086458510 hasConcept C153911025 @default.
- W2086458510 hasConcept C178790620 @default.
- W2086458510 hasConcept C185592680 @default.
- W2086458510 hasConcept C187882448 @default.
- W2086458510 hasConcept C19924922 @default.
- W2086458510 hasConcept C199360897 @default.
- W2086458510 hasConcept C2756471 @default.
- W2086458510 hasConcept C2777563447 @default.
- W2086458510 hasConcept C2779186596 @default.
- W2086458510 hasConcept C2781440490 @default.
- W2086458510 hasConcept C41008148 @default.
- W2086458510 hasConcept C49105822 @default.
- W2086458510 hasConcept C52001828 @default.
- W2086458510 hasConcept C54355233 @default.
- W2086458510 hasConcept C552990157 @default.
- W2086458510 hasConcept C82381507 @default.
- W2086458510 hasConcept C86803240 @default.
- W2086458510 hasConcept C90583042 @default.
- W2086458510 hasConceptScore W2086458510C104317684 @default.
- W2086458510 hasConceptScore W2086458510C112456955 @default.
- W2086458510 hasConceptScore W2086458510C121050878 @default.
- W2086458510 hasConceptScore W2086458510C153911025 @default.
- W2086458510 hasConceptScore W2086458510C178790620 @default.
- W2086458510 hasConceptScore W2086458510C185592680 @default.
- W2086458510 hasConceptScore W2086458510C187882448 @default.
- W2086458510 hasConceptScore W2086458510C19924922 @default.
- W2086458510 hasConceptScore W2086458510C199360897 @default.
- W2086458510 hasConceptScore W2086458510C2756471 @default.
- W2086458510 hasConceptScore W2086458510C2777563447 @default.
- W2086458510 hasConceptScore W2086458510C2779186596 @default.
- W2086458510 hasConceptScore W2086458510C2781440490 @default.
- W2086458510 hasConceptScore W2086458510C41008148 @default.
- W2086458510 hasConceptScore W2086458510C49105822 @default.
- W2086458510 hasConceptScore W2086458510C52001828 @default.
- W2086458510 hasConceptScore W2086458510C54355233 @default.
- W2086458510 hasConceptScore W2086458510C552990157 @default.
- W2086458510 hasConceptScore W2086458510C82381507 @default.
- W2086458510 hasConceptScore W2086458510C86803240 @default.
- W2086458510 hasConceptScore W2086458510C90583042 @default.
- W2086458510 hasLocation W20864585101 @default.
- W2086458510 hasOpenAccess W2086458510 @default.
- W2086458510 hasPrimaryLocation W20864585101 @default.
- W2086458510 hasRelatedWork W1828691184 @default.
- W2086458510 hasRelatedWork W1920751942 @default.
- W2086458510 hasRelatedWork W1992647879 @default.
- W2086458510 hasRelatedWork W2002128513 @default.
- W2086458510 hasRelatedWork W2020824267 @default.
- W2086458510 hasRelatedWork W2085192631 @default.
- W2086458510 hasRelatedWork W2119103177 @default.
- W2086458510 hasRelatedWork W2154141796 @default.
- W2086458510 hasRelatedWork W259946526 @default.
- W2086458510 hasRelatedWork W2092874662 @default.
- W2086458510 isParatext "false" @default.
- W2086458510 isRetracted "false" @default.
- W2086458510 magId "2086458510" @default.
- W2086458510 workType "article" @default.