Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086481868> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2086481868 endingPage "7038" @default.
- W2086481868 startingPage "7030" @default.
- W2086481868 abstract "Oxidative damage of DNA by endogenously generated oxygen radicals contributes to the mutagenic process. Hydroxy, alkoxy, and peroxy radicals all have the potential to react with DNA, giving rise to strand breaks and potentially mutagenic oxidative base damage. Although reactions of the hydroxy radical with DNA have been well studied, far less is known about the reactivities of these other radicals with DNA and their mutation-inducing potential. Frequencies of DNA base modifications and strand break densities caused by peroxy radical (ROO*) oxidation were measured by glyoxal gel electrophoretic analysis. We report the spectrum of mutations induced in Escherichia coli upon transfection with peroxy radical treated DNA carrying the lacZ alpha gene as a reporter. Transfection of DNA exposed to micromolar amounts of peroxy radical resulted in a 30-fold increase in mutation frequency in non-SOS-inducible cells. Sequencing analysis of DNA isolated from mutants showed that among base substitution mutants 88% consisted of transversions at G, with a nearly equal number of G --> C and G --> T mutants. Transition mutations were rarely detected, in contrast to control experiments. Electrophoretic analysis of peroxy radical treated DNA exposed to NaOH, Nth, and Fpg proteins demonstrated that abasic sites are not formed to any detectable degree. The oxidative G lesions are sensitive to digestion by the Fpg protein. We were unable to detect the formation of 8-oxo-dG by HPLC/electrochemical analysis of peroxy radical oxidation of dG, suggesting that the G --> T transversions were not caused by this base lesion." @default.
- W2086481868 created "2016-06-24" @default.
- W2086481868 creator A5035999555 @default.
- W2086481868 creator A5037061130 @default.
- W2086481868 creator A5077231429 @default.
- W2086481868 date "1998-04-22" @default.
- W2086481868 modified "2023-10-18" @default.
- W2086481868 title "Mutagenesis by Peroxy Radical Is Dominated by Transversions at Deoxyguanosine: Evidence for the Lack of Involvement of 8-oxo-dG<sup>1</sup> and/or Abasic Site Formation" @default.
- W2086481868 cites W152479649 @default.
- W2086481868 cites W1570917001 @default.
- W2086481868 cites W1599053981 @default.
- W2086481868 cites W1981503162 @default.
- W2086481868 cites W1983496733 @default.
- W2086481868 cites W1997802967 @default.
- W2086481868 cites W2035488328 @default.
- W2086481868 cites W2059639003 @default.
- W2086481868 cites W2109407065 @default.
- W2086481868 doi "https://doi.org/10.1021/bi973132m" @default.
- W2086481868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9578591" @default.
- W2086481868 hasPublicationYear "1998" @default.
- W2086481868 type Work @default.
- W2086481868 sameAs 2086481868 @default.
- W2086481868 citedByCount "33" @default.
- W2086481868 countsByYear W20864818682013 @default.
- W2086481868 countsByYear W20864818682014 @default.
- W2086481868 countsByYear W20864818682015 @default.
- W2086481868 countsByYear W20864818682017 @default.
- W2086481868 countsByYear W20864818682021 @default.
- W2086481868 crossrefType "journal-article" @default.
- W2086481868 hasAuthorship W2086481868A5035999555 @default.
- W2086481868 hasAuthorship W2086481868A5037061130 @default.
- W2086481868 hasAuthorship W2086481868A5077231429 @default.
- W2086481868 hasConcept C104317684 @default.
- W2086481868 hasConcept C139066938 @default.
- W2086481868 hasConcept C143065580 @default.
- W2086481868 hasConcept C143425029 @default.
- W2086481868 hasConcept C150777479 @default.
- W2086481868 hasConcept C153911025 @default.
- W2086481868 hasConcept C16318435 @default.
- W2086481868 hasConcept C185581394 @default.
- W2086481868 hasConcept C185592680 @default.
- W2086481868 hasConcept C192396546 @default.
- W2086481868 hasConcept C2776778087 @default.
- W2086481868 hasConcept C2780441211 @default.
- W2086481868 hasConcept C552990157 @default.
- W2086481868 hasConcept C55493867 @default.
- W2086481868 hasConcept C86803240 @default.
- W2086481868 hasConceptScore W2086481868C104317684 @default.
- W2086481868 hasConceptScore W2086481868C139066938 @default.
- W2086481868 hasConceptScore W2086481868C143065580 @default.
- W2086481868 hasConceptScore W2086481868C143425029 @default.
- W2086481868 hasConceptScore W2086481868C150777479 @default.
- W2086481868 hasConceptScore W2086481868C153911025 @default.
- W2086481868 hasConceptScore W2086481868C16318435 @default.
- W2086481868 hasConceptScore W2086481868C185581394 @default.
- W2086481868 hasConceptScore W2086481868C185592680 @default.
- W2086481868 hasConceptScore W2086481868C192396546 @default.
- W2086481868 hasConceptScore W2086481868C2776778087 @default.
- W2086481868 hasConceptScore W2086481868C2780441211 @default.
- W2086481868 hasConceptScore W2086481868C552990157 @default.
- W2086481868 hasConceptScore W2086481868C55493867 @default.
- W2086481868 hasConceptScore W2086481868C86803240 @default.
- W2086481868 hasIssue "19" @default.
- W2086481868 hasLocation W20864818681 @default.
- W2086481868 hasLocation W20864818682 @default.
- W2086481868 hasOpenAccess W2086481868 @default.
- W2086481868 hasPrimaryLocation W20864818681 @default.
- W2086481868 hasRelatedWork W1544553682 @default.
- W2086481868 hasRelatedWork W1969117816 @default.
- W2086481868 hasRelatedWork W2003490882 @default.
- W2086481868 hasRelatedWork W2016585187 @default.
- W2086481868 hasRelatedWork W2021312971 @default.
- W2086481868 hasRelatedWork W2042743402 @default.
- W2086481868 hasRelatedWork W2045038845 @default.
- W2086481868 hasRelatedWork W2061972729 @default.
- W2086481868 hasRelatedWork W2067520564 @default.
- W2086481868 hasRelatedWork W2086481868 @default.
- W2086481868 hasVolume "37" @default.
- W2086481868 isParatext "false" @default.
- W2086481868 isRetracted "false" @default.
- W2086481868 magId "2086481868" @default.
- W2086481868 workType "article" @default.