SemOpenAlex |

SemOpenAlex

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086569215> ?p ?o ?g. }

Showing items 1 to 100 of ±111 with 100 items per page.

W2086569215 endingPage "8189" @default.
W2086569215 startingPage "8182" @default.
W2086569215 abstract "The study of proteinase expression in crude extracts from different organs of the globe artichoke (Cynara scolymus L.) disclosed that enzymes with proteolytic and milk-clotting activity are mainly located in mature flowers. Maximum proteolytic activity was recorded at pH 5.0, and inhibition studies showed that only pepstatin, specific for aspartic proteinases, presented a significant inhibitory effect. Such properties, in addition to easy enzyme inactivation by moderate heating, make this crude protease extract potentially useful for cheese production. Adsorption with activated carbon, together with anion exchange and affinity chromatography, led to the isolation of a heterodimeric milk-clotting proteinase consisting of 30- and 15-kDa subunits. MALDI-TOF MS of the 15-kDa chain determined a 15.358-Da mass, and the terminal amino sequence presented 96% homology with the smaller cardosin A subunit. The amino terminal sequence of the 30-kDa chain proved to be identical to the larger cardosin A subunit. Electrophoresis evidenced proteinase self-processing that was confirmed by immunoblots presenting 62-, 30-, and 15-kDa bands." @default.
W2086569215 created "2016-06-24" @default.
W2086569215 creator A5043527786 @default.
W2086569215 creator A5056264345 @default.
W2086569215 creator A5086964197 @default.
W2086569215 date "2004-11-24" @default.
W2086569215 modified "2023-10-12" @default.
W2086569215 title "Purification and Characterization of a Milk-Clotting Aspartic Proteinase from Globe Artichoke (<i>Cynara scolymus </i>L.)" @default.
W2086569215 cites W1495702220 @default.
W2086569215 cites W1496647822 @default.
W2086569215 cites W1503980501 @default.
W2086569215 cites W1964677613 @default.
W2086569215 cites W1968471406 @default.
W2086569215 cites W1969930485 @default.
W2086569215 cites W1970391112 @default.
W2086569215 cites W1974020136 @default.
W2086569215 cites W2006074123 @default.
W2086569215 cites W2008076410 @default.
W2086569215 cites W2013110864 @default.
W2086569215 cites W2028106597 @default.
W2086569215 cites W2029088519 @default.
W2086569215 cites W2032425238 @default.
W2086569215 cites W2032999827 @default.
W2086569215 cites W2043578428 @default.
W2086569215 cites W2062549262 @default.
W2086569215 cites W2083699890 @default.
W2086569215 cites W2086022519 @default.
W2086569215 cites W2086170364 @default.
W2086569215 cites W2086512538 @default.
W2086569215 cites W2091095874 @default.
W2086569215 cites W2094580359 @default.
W2086569215 cites W2099265131 @default.
W2086569215 cites W2100837269 @default.
W2086569215 cites W2103751801 @default.
W2086569215 cites W2142685726 @default.
W2086569215 cites W2158714788 @default.
W2086569215 cites W2165933816 @default.
W2086569215 cites W2167590641 @default.
W2086569215 cites W4293247451 @default.
W2086569215 doi "https://doi.org/10.1021/jf049006o" @default.
W2086569215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15612815" @default.
W2086569215 hasPublicationYear "2004" @default.
W2086569215 type Work @default.
W2086569215 sameAs 2086569215 @default.
W2086569215 citedByCount "58" @default.
W2086569215 countsByYear W20865692152012 @default.
W2086569215 countsByYear W20865692152013 @default.
W2086569215 countsByYear W20865692152014 @default.
W2086569215 countsByYear W20865692152015 @default.
W2086569215 countsByYear W20865692152016 @default.
W2086569215 countsByYear W20865692152017 @default.
W2086569215 countsByYear W20865692152018 @default.
W2086569215 countsByYear W20865692152019 @default.
W2086569215 countsByYear W20865692152020 @default.
W2086569215 countsByYear W20865692152021 @default.
W2086569215 countsByYear W20865692152022 @default.
W2086569215 countsByYear W20865692152023 @default.
W2086569215 crossrefType "journal-article" @default.
W2086569215 hasAuthorship W2086569215A5043527786 @default.
W2086569215 hasAuthorship W2086569215A5056264345 @default.
W2086569215 hasAuthorship W2086569215A5086964197 @default.
W2086569215 hasConcept C104292427 @default.
W2086569215 hasConcept C104317684 @default.
W2086569215 hasConcept C181199279 @default.
W2086569215 hasConcept C185592680 @default.
W2086569215 hasConcept C2776350231 @default.
W2086569215 hasConcept C2776714187 @default.
W2086569215 hasConcept C2776738293 @default.
W2086569215 hasConcept C2777548131 @default.
W2086569215 hasConcept C2781307694 @default.
W2086569215 hasConcept C3019223276 @default.
W2086569215 hasConcept C55493867 @default.
W2086569215 hasConcept C58121356 @default.
W2086569215 hasConcept C59822182 @default.
W2086569215 hasConcept C86803240 @default.
W2086569215 hasConceptScore W2086569215C104292427 @default.
W2086569215 hasConceptScore W2086569215C104317684 @default.
W2086569215 hasConceptScore W2086569215C181199279 @default.
W2086569215 hasConceptScore W2086569215C185592680 @default.
W2086569215 hasConceptScore W2086569215C2776350231 @default.
W2086569215 hasConceptScore W2086569215C2776714187 @default.
W2086569215 hasConceptScore W2086569215C2776738293 @default.
W2086569215 hasConceptScore W2086569215C2777548131 @default.
W2086569215 hasConceptScore W2086569215C2781307694 @default.
W2086569215 hasConceptScore W2086569215C3019223276 @default.
W2086569215 hasConceptScore W2086569215C55493867 @default.
W2086569215 hasConceptScore W2086569215C58121356 @default.
W2086569215 hasConceptScore W2086569215C59822182 @default.
W2086569215 hasConceptScore W2086569215C86803240 @default.
W2086569215 hasIssue "26" @default.
W2086569215 hasLocation W20865692151 @default.
W2086569215 hasLocation W20865692152 @default.
W2086569215 hasOpenAccess W2086569215 @default.
W2086569215 hasPrimaryLocation W20865692151 @default.
W2086569215 hasRelatedWork W2009610982 @default.
W2086569215 hasRelatedWork W2023041431 @default.
W2086569215 hasRelatedWork W2026449352 @default.
W2086569215 hasRelatedWork W2036793446 @default.