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- W2086618924 abstract "Cell swelling takes place rapidly when animal cells in monolayer culture are treated with hypotonic buffer in situ; scraping of the swollen cells causes virtually 100% cell lysis. Because this procedure avoids the use of scraping and centrifugation to collect the cells and the use of Dounce homogenizers for cell disruption, recovery of the cell extract is very high. 3-Hydroxy-3-methylglutaryl coenzyme A reductase activities of cell extracts prepared by this method are virtually identical to those prepared by the conventional procedure involving Dounce homogenization." @default.
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- W2086618924 date "1981-09-01" @default.
- W2086618924 modified "2023-10-17" @default.
- W2086618924 title "A simple and efficient procedure for the rapid homogenization of cultured animal cells grown in monolayer" @default.
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- W2086618924 doi "https://doi.org/10.1016/0003-2697(81)90360-2" @default.
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