FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086636612> ?p ?o ?g. }

• W2086636612 endingPage "5294" @default.
• W2086636612 startingPage "5279" @default.
• W2086636612 abstract "Severe acute respiratory syndrome (SARS) coronavirus infection and growth are dependent on initiating signaling and enzyme actions upon viral entry into the host cell. Proteins packaged during virus assembly may subsequently form the first line of attack and host manipulation upon infection. A complete characterization of virion components is therefore important to understanding the dynamics of early stages of infection. Mass spectrometry and kinase profiling techniques identified nearly 200 incorporated host and viral proteins. We used published interaction data to identify hubs of connectivity with potential significance for virion formation. Surprisingly, the hub with the most potential connections was not the viral M protein but the nonstructural protein 3 (nsp3), which is one of the novel virion components identified by mass spectrometry. Based on new experimental data and a bioinformatics analysis across the Coronaviridae, we propose a higher-resolution functional domain architecture for nsp3 that determines the interaction capacity of this protein. Using recombinant protein domains expressed in Escherichia coli, we identified two additional RNA-binding domains of nsp3. One of these domains is located within the previously described SARS-unique domain, and there is a nucleic acid chaperone-like domain located immediately downstream of the papain-like proteinase domain. We also identified a novel cysteine-coordinated metal ion-binding domain. Analyses of interdomain interactions and provisional functional annotation of the remaining, so-far-uncharacterized domains are presented. Overall, the ensemble of data surveyed here paint a more complete picture of nsp3 as a conserved component of the viral protein processing machinery, which is intimately associated with viral RNA in its role as a virion component." @default.
• W2086636612 created "2016-06-24" @default.
• W2086636612 creator A5000254356 @default.
• W2086636612 creator A5000472988 @default.
• W2086636612 creator A5008173217 @default.
• W2086636612 creator A5017648766 @default.
• W2086636612 creator A5026024070 @default.
• W2086636612 creator A5027073037 @default.
• W2086636612 creator A5035418647 @default.
• W2086636612 creator A5038725535 @default.
• W2086636612 creator A5040229329 @default.
• W2086636612 creator A5078239117 @default.
• W2086636612 creator A5083768832 @default.
• W2086636612 creator A5086304347 @default.
• W2086636612 creator A5088717384 @default.
• W2086636612 date "2008-06-01" @default.
• W2086636612 modified "2023-09-30" @default.
• W2086636612 title "Proteomics Analysis Unravels the Functional Repertoire of Coronavirus Nonstructural Protein 3" @default.
• W2086636612 cites W1491556421 @default.
• W2086636612 cites W1491787713 @default.
• W2086636612 cites W1544561280 @default.
• W2086636612 cites W1563731295 @default.
• W2086636612 cites W1597678601 @default.
• W2086636612 cites W1756426621 @default.
• W2086636612 cites W1791897256 @default.
• W2086636612 cites W1832172949 @default.
• W2086636612 cites W1902920842 @default.
• W2086636612 cites W1968574388 @default.
• W2086636612 cites W1970696723 @default.
• W2086636612 cites W1971168306 @default.
• W2086636612 cites W1983495708 @default.
• W2086636612 cites W1989424501 @default.
• W2086636612 cites W1990299806 @default.
• W2086636612 cites W1990649962 @default.
• W2086636612 cites W1994279587 @default.
• W2086636612 cites W2003852785 @default.
• W2086636612 cites W2008975313 @default.
• W2086636612 cites W2009782062 @default.
• W2086636612 cites W2019184993 @default.
• W2086636612 cites W2019312368 @default.
• W2086636612 cites W2022879707 @default.
• W2086636612 cites W2026465178 @default.
• W2086636612 cites W2027371255 @default.
• W2086636612 cites W2037665967 @default.
• W2086636612 cites W2038096428 @default.
• W2086636612 cites W2040462912 @default.
• W2086636612 cites W2045340223 @default.
• W2086636612 cites W2048627201 @default.
• W2086636612 cites W2050996246 @default.
• W2086636612 cites W2055043387 @default.
• W2086636612 cites W2062041529 @default.
• W2086636612 cites W2067933199 @default.
• W2086636612 cites W2068921257 @default.
• W2086636612 cites W2072528578 @default.
• W2086636612 cites W2076484749 @default.
• W2086636612 cites W2078256648 @default.
• W2086636612 cites W2081309601 @default.
• W2086636612 cites W2087800735 @default.
• W2086636612 cites W2092267439 @default.
• W2086636612 cites W2092581586 @default.
• W2086636612 cites W2093392569 @default.
• W2086636612 cites W2095724872 @default.
• W2086636612 cites W2100593398 @default.
• W2086636612 cites W2109027260 @default.
• W2086636612 cites W2110863368 @default.
• W2086636612 cites W2113927019 @default.
• W2086636612 cites W2114646589 @default.
• W2086636612 cites W2115897082 @default.
• W2086636612 cites W2116160060 @default.
• W2086636612 cites W2120660611 @default.
• W2086636612 cites W2126245412 @default.
• W2086636612 cites W2127226387 @default.
• W2086636612 cites W2127531900 @default.
• W2086636612 cites W2129482492 @default.
• W2086636612 cites W2134168236 @default.
• W2086636612 cites W2135337307 @default.
• W2086636612 cites W2135537539 @default.
• W2086636612 cites W2138929325 @default.
• W2086636612 cites W2139682436 @default.
• W2086636612 cites W2140471782 @default.
• W2086636612 cites W2141959911 @default.
• W2086636612 cites W2145329041 @default.
• W2086636612 cites W2148979869 @default.
• W2086636612 cites W2149301799 @default.
• W2086636612 cites W2149753575 @default.
• W2086636612 cites W2152770371 @default.
• W2086636612 cites W2163480486 @default.
• W2086636612 cites W2164466981 @default.
• W2086636612 cites W2168341495 @default.
• W2086636612 cites W2171291163 @default.
• W2086636612 doi "https://doi.org/10.1128/jvi.02631-07" @default.
• W2086636612 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2395186" @default.
• W2086636612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18367524" @default.
• W2086636612 hasPublicationYear "2008" @default.
• W2086636612 type Work @default.
• W2086636612 sameAs 2086636612 @default.
• W2086636612 citedByCount "166" @default.
• W2086636612 countsByYear W20866366122012 @default.

• next