FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086653054> ?p ?o ?g. }

• W2086653054 endingPage "1645" @default.
• W2086653054 startingPage "1634" @default.
• W2086653054 abstract "We have used an indane-dione spin label (2-[-oxyl-2,2,5,5-tetramethyl-3-pyrrolin-3-yl)methenyl]in dane-1,3-dione), designated InVSL, to study the orientation of myosin heads in bundles of chemically skinned rabbit psoas muscle fibers, with electron paramagnetic resonance (EPR) spectroscopy. After reversible preblocking with 5,5'-dithiobis(2-nitro-benzoic acid) (DTNB), we were able to attach most of the spin label covalently and rigidly to either Cys 707 (SH1) or Cys 697 (SH2) on myosin heads. EPR spectra of labeled fibers contained substantial contributions from both oriented and disordered populations of spin labels. Similar spectra were obtained from fibers decorated with InVSL-labeled myosin heads (subfragment 1), indicating that virtually all the spin labels in labeled fibers are on the myosin head. We specifically labeled SH2 with InVSL after reversible preblocking of the SH1 sites with 1-fluoro-2,4-dinitrobenzene (FDNB), resulting in a spectrum that indicated only disordered spin labels. Therefore, the oriented and disordered populations correspond to labels on SH1 and SH2, respectively. The spectrum of SH2-bound labels was subtracted to produce a spectrum corresponding to SH1-bound labels, which was used for further analysis. For this corrected spectrum, the angle between the fiber axis and the principal axis of the spin label was fitted well by a Gaussian distribution centered at theta o = 11 +/- 1 degree, with a full width at half-maximum of delta theta = 15 +/- 2 degrees. The unique orientation of InVSL, with its principal axis almost parallel to the fiber axis, makes it complementary to spin labels previously studied in this system. This label can provide unambiguous information about axial rotations of myosin heads, since any axial rotation of the head must be reflected in the same axial rotation of the principal axis of the probe, thus changing the hyperfine splitting. Therefore, InVSL-labeled fibers have ideal properties needed for further exploration myosin head orientation and rotational motion in muscle." @default.
• W2086653054 created "2016-06-24" @default.
• W2086653054 creator A5023379990 @default.
• W2086653054 creator A5089936943 @default.
• W2086653054 date "1994-10-01" @default.
• W2086653054 modified "2023-10-16" @default.
• W2086653054 title "A spin label that binds to myosin heads in muscle fibers with its principal axis parallel to the fiber axis" @default.
• W2086653054 cites W1569410959 @default.
• W2086653054 cites W170266664 @default.
• W2086653054 cites W1759875777 @default.
• W2086653054 cites W1982718021 @default.
• W2086653054 cites W1988311328 @default.
• W2086653054 cites W1997568741 @default.
• W2086653054 cites W2006814619 @default.
• W2086653054 cites W2010180393 @default.
• W2086653054 cites W2017851912 @default.
• W2086653054 cites W2022465751 @default.
• W2086653054 cites W2029802074 @default.
• W2086653054 cites W2032802380 @default.
• W2086653054 cites W2038877333 @default.
• W2086653054 cites W2041909694 @default.
• W2086653054 cites W2044629144 @default.
• W2086653054 cites W2048453107 @default.
• W2086653054 cites W2051946285 @default.
• W2086653054 cites W2059820101 @default.
• W2086653054 cites W2062773727 @default.
• W2086653054 cites W2069953906 @default.
• W2086653054 cites W2072529314 @default.
• W2086653054 cites W2087837838 @default.
• W2086653054 cites W2089802914 @default.
• W2086653054 cites W2098175385 @default.
• W2086653054 cites W2127917887 @default.
• W2086653054 cites W2142650206 @default.
• W2086653054 cites W2164676813 @default.
• W2086653054 cites W2218720641 @default.
• W2086653054 cites W2346166802 @default.
• W2086653054 doi "https://doi.org/10.1016/s0006-3495(94)80636-8" @default.
• W2086653054 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1225525" @default.
• W2086653054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7819495" @default.
• W2086653054 hasPublicationYear "1994" @default.
• W2086653054 type Work @default.
• W2086653054 sameAs 2086653054 @default.
• W2086653054 citedByCount "17" @default.
• W2086653054 countsByYear W20866530542012 @default.
• W2086653054 countsByYear W20866530542013 @default.
• W2086653054 crossrefType "journal-article" @default.
• W2086653054 hasAuthorship W2086653054A5023379990 @default.
• W2086653054 hasAuthorship W2086653054A5089936943 @default.
• W2086653054 hasBestOaLocation W20866530541 @default.
• W2086653054 hasConcept C121332964 @default.
• W2086653054 hasConcept C12554922 @default.
• W2086653054 hasConcept C15954220 @default.
• W2086653054 hasConcept C178790620 @default.
• W2086653054 hasConcept C185592680 @default.
• W2086653054 hasConcept C187961010 @default.
• W2086653054 hasConcept C46141821 @default.
• W2086653054 hasConcept C519885992 @default.
• W2086653054 hasConcept C55493867 @default.
• W2086653054 hasConcept C6997183 @default.
• W2086653054 hasConcept C71240020 @default.
• W2086653054 hasConcept C79123411 @default.
• W2086653054 hasConcept C8010536 @default.
• W2086653054 hasConcept C8035138 @default.
• W2086653054 hasConcept C86803240 @default.
• W2086653054 hasConceptScore W2086653054C121332964 @default.
• W2086653054 hasConceptScore W2086653054C12554922 @default.
• W2086653054 hasConceptScore W2086653054C15954220 @default.
• W2086653054 hasConceptScore W2086653054C178790620 @default.
• W2086653054 hasConceptScore W2086653054C185592680 @default.
• W2086653054 hasConceptScore W2086653054C187961010 @default.
• W2086653054 hasConceptScore W2086653054C46141821 @default.
• W2086653054 hasConceptScore W2086653054C519885992 @default.
• W2086653054 hasConceptScore W2086653054C55493867 @default.
• W2086653054 hasConceptScore W2086653054C6997183 @default.
• W2086653054 hasConceptScore W2086653054C71240020 @default.
• W2086653054 hasConceptScore W2086653054C79123411 @default.
• W2086653054 hasConceptScore W2086653054C8010536 @default.
• W2086653054 hasConceptScore W2086653054C8035138 @default.
• W2086653054 hasConceptScore W2086653054C86803240 @default.
• W2086653054 hasIssue "4" @default.
• W2086653054 hasLocation W20866530541 @default.
• W2086653054 hasLocation W20866530542 @default.
• W2086653054 hasLocation W20866530543 @default.
• W2086653054 hasLocation W20866530544 @default.
• W2086653054 hasOpenAccess W2086653054 @default.
• W2086653054 hasPrimaryLocation W20866530541 @default.
• W2086653054 hasRelatedWork W1981646615 @default.
• W2086653054 hasRelatedWork W1994466407 @default.
• W2086653054 hasRelatedWork W2005214922 @default.
• W2086653054 hasRelatedWork W2080594424 @default.
• W2086653054 hasRelatedWork W2083911985 @default.
• W2086653054 hasRelatedWork W2087573052 @default.
• W2086653054 hasRelatedWork W2131202031 @default.
• W2086653054 hasRelatedWork W2144321597 @default.
• W2086653054 hasRelatedWork W4289222729 @default.
• W2086653054 hasRelatedWork W4318026684 @default.
• W2086653054 hasVolume "67" @default.
• W2086653054 isParatext "false" @default.

• next