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- W2086693742 abstract "The fusion of DNA fragments is becoming increasingly more important. The ability to work without being constrained by restriction sites enables DNA fusion to be applied to a much broader range of fragments. Therefore, we describe a simplified polymerase chain reaction (PCR)-based method for fusion of DNA fragments in one step. In a single PCR, two templates, an overlapping primer, and template-specific forward and reverse primers are used. After a few cycles, the fusion DNA is assembled and is amplified. The ratio of overlapping primer to forward/reverse primers and template DNA is essential for the success of the reaction." @default.
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- W2086693742 date "2012-02-01" @default.
- W2086693742 modified "2023-09-27" @default.
- W2086693742 title "One-pot fusion polymerase chain reaction for combinatorial synthesis of DNA from several cassettes" @default.
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- W2086693742 doi "https://doi.org/10.1016/j.ab.2011.12.027" @default.
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