Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086697071> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2086697071 abstract "Protein engineering has been used to identify residues of the tyrosyl-tRNA synthetase from Bacillus stearothermophilus that are in contact with the tRNA Tyr . By using improved techniques in oligonucleotide-directed mutagenesis, forty lysine, arginine, or histidine residues on the surface of the enzyme were altered to either asparagine or glutamine. With an in vivo genetic complementation test, only thirteen mutants were found that seriously affect the overall activity of the enzyme. Detailed kinetics on the purified enzymes revealed that four of these mutants had a lesion at the level of the activation of tyrosine, and nine at the level of tRNA charging. Three of the mutants in tRNA charging lie in the N-terminal domain of the enzyme which is responsible for tyrosine activation, and the six others in the disordered C-terminal domain which is necessary for tRNA binding. This indicates that the tRNA spans both domains of the enzyme. The construction of heterodimers allows us to suggest a model for tRNA binding in which the acceptor stem of the tRNA binds to the N-terminal domain of one subunit, and other regions of tRNA Tyr such as the anticodon arm or extra loop bind to the C-terminal domain of the other subunit." @default.
- W2086697071 created "2016-06-24" @default.
- W2086697071 creator A5011884737 @default.
- W2086697071 creator A5046268671 @default.
- W2086697071 creator A5047646174 @default.
- W2086697071 date "1986-04-30" @default.
- W2086697071 modified "2023-09-27" @default.
- W2086697071 title "Protein engineering of tyrosyl-tRNA synthetase: the charging of tRNA" @default.
- W2086697071 cites W1970799706 @default.
- W2086697071 cites W1972989133 @default.
- W2086697071 cites W1993606408 @default.
- W2086697071 cites W2007651040 @default.
- W2086697071 cites W2015931172 @default.
- W2086697071 cites W2021853870 @default.
- W2086697071 cites W2035650458 @default.
- W2086697071 cites W2042748071 @default.
- W2086697071 cites W2054507895 @default.
- W2086697071 cites W2066180422 @default.
- W2086697071 cites W2149847431 @default.
- W2086697071 cites W2229547508 @default.
- W2086697071 cites W2482076468 @default.
- W2086697071 doi "https://doi.org/10.1098/rsta.1986.0053" @default.
- W2086697071 hasPublicationYear "1986" @default.
- W2086697071 type Work @default.
- W2086697071 sameAs 2086697071 @default.
- W2086697071 citedByCount "3" @default.
- W2086697071 crossrefType "journal-article" @default.
- W2086697071 hasAuthorship W2086697071A5011884737 @default.
- W2086697071 hasAuthorship W2086697071A5046268671 @default.
- W2086697071 hasAuthorship W2086697071A5047646174 @default.
- W2086697071 hasConcept C104317684 @default.
- W2086697071 hasConcept C143065580 @default.
- W2086697071 hasConcept C153957851 @default.
- W2086697071 hasConcept C16318435 @default.
- W2086697071 hasConcept C181199279 @default.
- W2086697071 hasConcept C185592680 @default.
- W2086697071 hasConcept C188082640 @default.
- W2086697071 hasConcept C21312847 @default.
- W2086697071 hasConcept C2775895851 @default.
- W2086697071 hasConcept C2776165026 @default.
- W2086697071 hasConcept C2778460671 @default.
- W2086697071 hasConcept C2779315393 @default.
- W2086697071 hasConcept C55493867 @default.
- W2086697071 hasConcept C67705224 @default.
- W2086697071 hasConcept C86803240 @default.
- W2086697071 hasConceptScore W2086697071C104317684 @default.
- W2086697071 hasConceptScore W2086697071C143065580 @default.
- W2086697071 hasConceptScore W2086697071C153957851 @default.
- W2086697071 hasConceptScore W2086697071C16318435 @default.
- W2086697071 hasConceptScore W2086697071C181199279 @default.
- W2086697071 hasConceptScore W2086697071C185592680 @default.
- W2086697071 hasConceptScore W2086697071C188082640 @default.
- W2086697071 hasConceptScore W2086697071C21312847 @default.
- W2086697071 hasConceptScore W2086697071C2775895851 @default.
- W2086697071 hasConceptScore W2086697071C2776165026 @default.
- W2086697071 hasConceptScore W2086697071C2778460671 @default.
- W2086697071 hasConceptScore W2086697071C2779315393 @default.
- W2086697071 hasConceptScore W2086697071C55493867 @default.
- W2086697071 hasConceptScore W2086697071C67705224 @default.
- W2086697071 hasConceptScore W2086697071C86803240 @default.
- W2086697071 hasLocation W20866970711 @default.
- W2086697071 hasOpenAccess W2086697071 @default.
- W2086697071 hasPrimaryLocation W20866970711 @default.
- W2086697071 hasRelatedWork W1533192201 @default.
- W2086697071 hasRelatedWork W1963927157 @default.
- W2086697071 hasRelatedWork W1989835479 @default.
- W2086697071 hasRelatedWork W1989957957 @default.
- W2086697071 hasRelatedWork W1992401304 @default.
- W2086697071 hasRelatedWork W1992746198 @default.
- W2086697071 hasRelatedWork W1992932889 @default.
- W2086697071 hasRelatedWork W2005785049 @default.
- W2086697071 hasRelatedWork W2008335672 @default.
- W2086697071 hasRelatedWork W2024420567 @default.
- W2086697071 hasRelatedWork W2039946498 @default.
- W2086697071 hasRelatedWork W2043927602 @default.
- W2086697071 hasRelatedWork W2049656887 @default.
- W2086697071 hasRelatedWork W2064190025 @default.
- W2086697071 hasRelatedWork W2067273363 @default.
- W2086697071 hasRelatedWork W2069575698 @default.
- W2086697071 hasRelatedWork W2090096169 @default.
- W2086697071 hasRelatedWork W2229547508 @default.
- W2086697071 hasRelatedWork W2975684156 @default.
- W2086697071 hasRelatedWork W3023847667 @default.
- W2086697071 isParatext "false" @default.
- W2086697071 isRetracted "false" @default.
- W2086697071 magId "2086697071" @default.
- W2086697071 workType "article" @default.