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• W2086731598 abstract "Purpose/Objective(s)To examine whether carbon ion beam alone or in combination with gemcitabine and/or cisplatin is effective in targeting putative human cholangiocarcinoma stem cells.Materials/MethodsPutative cancer stem cells (CSCs) sorted from HuCCT1 and Huh28 cells were treated with or without carbon ion beam, X-ray alone or in combination with gemcitabine and/or cisplatin, then colony and spheroid formation assay, FACS analysis, gamma-H2AX foci assay were performed.ResultsColony and spheroid formation assay as well as in vivo tumorigenic assay showed that CD44+/ESA+, CD133+/CD90+ cells exactly have CSC properties compared to CD44-/ESA- and CD133-/CD90- cells. The proportion of CD44+/ESA+, CD133+/CD90+ was increased by X-ray compared to carbon-ion beam alone, and it was further enhanced when combine with gemcitabine and/or cisplatin. The number of colony and spheroid formed from CSCs was remarkably reduced after carbon ion beam combined with gemcitabine and/or cisplatin compared to that of X-ray or carbon ion beam alone. A more large number and large-sized gamma-H2AX foci were observed in CSCs after 24 h carbon ion beam combined with gemcitabine and/or cisplatin compared to that of X-ray or carbon ion beam alone.ConclusionsCarbon ion beam combined with gemcitabine and/or cisplatin effectively induced prolonged and more complex DNA damage, as a result effectively destroyed cholangiocarcinoma stem cells. Purpose/Objective(s)To examine whether carbon ion beam alone or in combination with gemcitabine and/or cisplatin is effective in targeting putative human cholangiocarcinoma stem cells. To examine whether carbon ion beam alone or in combination with gemcitabine and/or cisplatin is effective in targeting putative human cholangiocarcinoma stem cells. Materials/MethodsPutative cancer stem cells (CSCs) sorted from HuCCT1 and Huh28 cells were treated with or without carbon ion beam, X-ray alone or in combination with gemcitabine and/or cisplatin, then colony and spheroid formation assay, FACS analysis, gamma-H2AX foci assay were performed. Putative cancer stem cells (CSCs) sorted from HuCCT1 and Huh28 cells were treated with or without carbon ion beam, X-ray alone or in combination with gemcitabine and/or cisplatin, then colony and spheroid formation assay, FACS analysis, gamma-H2AX foci assay were performed. ResultsColony and spheroid formation assay as well as in vivo tumorigenic assay showed that CD44+/ESA+, CD133+/CD90+ cells exactly have CSC properties compared to CD44-/ESA- and CD133-/CD90- cells. The proportion of CD44+/ESA+, CD133+/CD90+ was increased by X-ray compared to carbon-ion beam alone, and it was further enhanced when combine with gemcitabine and/or cisplatin. The number of colony and spheroid formed from CSCs was remarkably reduced after carbon ion beam combined with gemcitabine and/or cisplatin compared to that of X-ray or carbon ion beam alone. A more large number and large-sized gamma-H2AX foci were observed in CSCs after 24 h carbon ion beam combined with gemcitabine and/or cisplatin compared to that of X-ray or carbon ion beam alone. Colony and spheroid formation assay as well as in vivo tumorigenic assay showed that CD44+/ESA+, CD133+/CD90+ cells exactly have CSC properties compared to CD44-/ESA- and CD133-/CD90- cells. The proportion of CD44+/ESA+, CD133+/CD90+ was increased by X-ray compared to carbon-ion beam alone, and it was further enhanced when combine with gemcitabine and/or cisplatin. The number of colony and spheroid formed from CSCs was remarkably reduced after carbon ion beam combined with gemcitabine and/or cisplatin compared to that of X-ray or carbon ion beam alone. A more large number and large-sized gamma-H2AX foci were observed in CSCs after 24 h carbon ion beam combined with gemcitabine and/or cisplatin compared to that of X-ray or carbon ion beam alone. ConclusionsCarbon ion beam combined with gemcitabine and/or cisplatin effectively induced prolonged and more complex DNA damage, as a result effectively destroyed cholangiocarcinoma stem cells. Carbon ion beam combined with gemcitabine and/or cisplatin effectively induced prolonged and more complex DNA damage, as a result effectively destroyed cholangiocarcinoma stem cells." @default.
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• W2086731598 date "2014-09-01" @default.
• W2086731598 modified "2023-10-18" @default.
• W2086731598 title "Effects of Carbon Ion Beam Alone or in Combination With Gemcitabine and/or Cisplatin on Cholangiocarcinoma Stem-Like Cells" @default.
• W2086731598 doi "https://doi.org/10.1016/j.ijrobp.2014.05.2277" @default.
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