Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086765935> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2086765935 endingPage "158" @default.
- W2086765935 startingPage "151" @default.
- W2086765935 abstract "Ligase chain reaction (LCR) is a useful molecular technique for detecting known point mutations. We report the first example of the use of a disposable silicon-glass micro-chip for LCR and thefirst application capillary electrophoresis (CE) to analyze samples amplified by LCR in a chip. Silicon-glass chips were manufactured using conventional photolithography and anodic bonding. The chips provide three distinct advantages for LCR: excellent thermal conductivity, a micro reaction volume (<10 μl), and reproducible,, low-cost manufacturing. Investigation and quantitation of amplification efficiency of LCR in a chip or in a tube requires an analytical technique that is faster and more convenient than the conventional slab gel methods. Slab gel electrophoresis uses relatively large amounts of sample and is labor-intensive and time-consuming, and thus is unsuitable for the separation and detection of LCR products. In contrast CE requires sample volumes (original LCR products) of less than 1 μl and is therefore well-suited to analysis of the micro-volume reaction mixture from chips. We combined CE with a sensitive laser induced fluorescence (LIF) detection system for the rapid separation and quantitative detection of LCR products amplified from the lacI gene in a silicon-glass chip. Comparative studies were made with LCR between tubes and silicon-glass chips. CE-LIF analysis is ideally suited to examination of micro-LCR amplification with high thoughput. The technologies may find medical uses in disease diagnosis and research." @default.
- W2086765935 created "2016-06-24" @default.
- W2086765935 creator A5022350572 @default.
- W2086765935 creator A5031286932 @default.
- W2086765935 creator A5032713644 @default.
- W2086765935 creator A5047523000 @default.
- W2086765935 creator A5053315447 @default.
- W2086765935 date "1996-04-01" @default.
- W2086765935 modified "2023-10-10" @default.
- W2086765935 title "Analysis of ligase chain reaction products amplified in a silicon-glass chip using capillary electrophoresis" @default.
- W2086765935 cites W1977236778 @default.
- W2086765935 cites W1986341687 @default.
- W2086765935 cites W1988270476 @default.
- W2086765935 cites W1990545420 @default.
- W2086765935 cites W1996027687 @default.
- W2086765935 cites W1998832343 @default.
- W2086765935 cites W2011069796 @default.
- W2086765935 cites W2021729201 @default.
- W2086765935 cites W2021747063 @default.
- W2086765935 cites W2023522315 @default.
- W2086765935 cites W2037729914 @default.
- W2086765935 cites W2040809244 @default.
- W2086765935 cites W2058093686 @default.
- W2086765935 cites W2062842274 @default.
- W2086765935 cites W2064063117 @default.
- W2086765935 cites W2086197143 @default.
- W2086765935 cites W2116210207 @default.
- W2086765935 cites W2127570331 @default.
- W2086765935 cites W2139814080 @default.
- W2086765935 cites W2460362987 @default.
- W2086765935 cites W2514396249 @default.
- W2086765935 cites W4245016883 @default.
- W2086765935 cites W4298158007 @default.
- W2086765935 doi "https://doi.org/10.1016/0021-9673(95)01257-5" @default.
- W2086765935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8646332" @default.
- W2086765935 hasPublicationYear "1996" @default.
- W2086765935 type Work @default.
- W2086765935 sameAs 2086765935 @default.
- W2086765935 citedByCount "63" @default.
- W2086765935 countsByYear W20867659352012 @default.
- W2086765935 countsByYear W20867659352015 @default.
- W2086765935 countsByYear W20867659352016 @default.
- W2086765935 countsByYear W20867659352019 @default.
- W2086765935 countsByYear W20867659352021 @default.
- W2086765935 crossrefType "journal-article" @default.
- W2086765935 hasAuthorship W2086765935A5022350572 @default.
- W2086765935 hasAuthorship W2086765935A5031286932 @default.
- W2086765935 hasAuthorship W2086765935A5032713644 @default.
- W2086765935 hasAuthorship W2086765935A5047523000 @default.
- W2086765935 hasAuthorship W2086765935A5053315447 @default.
- W2086765935 hasBestOaLocation W20867659351 @default.
- W2086765935 hasConcept C113196181 @default.
- W2086765935 hasConcept C119599485 @default.
- W2086765935 hasConcept C127413603 @default.
- W2086765935 hasConcept C159985019 @default.
- W2086765935 hasConcept C165005293 @default.
- W2086765935 hasConcept C178790620 @default.
- W2086765935 hasConcept C185592680 @default.
- W2086765935 hasConcept C192562407 @default.
- W2086765935 hasConcept C196806460 @default.
- W2086765935 hasConcept C2900643 @default.
- W2086765935 hasConcept C43617362 @default.
- W2086765935 hasConcept C544956773 @default.
- W2086765935 hasConceptScore W2086765935C113196181 @default.
- W2086765935 hasConceptScore W2086765935C119599485 @default.
- W2086765935 hasConceptScore W2086765935C127413603 @default.
- W2086765935 hasConceptScore W2086765935C159985019 @default.
- W2086765935 hasConceptScore W2086765935C165005293 @default.
- W2086765935 hasConceptScore W2086765935C178790620 @default.
- W2086765935 hasConceptScore W2086765935C185592680 @default.
- W2086765935 hasConceptScore W2086765935C192562407 @default.
- W2086765935 hasConceptScore W2086765935C196806460 @default.
- W2086765935 hasConceptScore W2086765935C2900643 @default.
- W2086765935 hasConceptScore W2086765935C43617362 @default.
- W2086765935 hasConceptScore W2086765935C544956773 @default.
- W2086765935 hasIssue "1" @default.
- W2086765935 hasLocation W20867659351 @default.
- W2086765935 hasLocation W20867659352 @default.
- W2086765935 hasOpenAccess W2086765935 @default.
- W2086765935 hasPrimaryLocation W20867659351 @default.
- W2086765935 hasRelatedWork W1493546459 @default.
- W2086765935 hasRelatedWork W1513019982 @default.
- W2086765935 hasRelatedWork W1608690192 @default.
- W2086765935 hasRelatedWork W2053617011 @default.
- W2086765935 hasRelatedWork W2347602094 @default.
- W2086765935 hasRelatedWork W2349989060 @default.
- W2086765935 hasRelatedWork W2392135368 @default.
- W2086765935 hasRelatedWork W3122375487 @default.
- W2086765935 hasRelatedWork W564135354 @default.
- W2086765935 hasRelatedWork W591383510 @default.
- W2086765935 hasVolume "732" @default.
- W2086765935 isParatext "false" @default.
- W2086765935 isRetracted "false" @default.
- W2086765935 magId "2086765935" @default.
- W2086765935 workType "article" @default.