FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086845471> ?p ?o ?g. }

• W2086845471 endingPage "297" @default.
• W2086845471 startingPage "292" @default.
• W2086845471 abstract "In this study, the efficacies of short hairpin RNAs (shRNAs) targeting different regions of West Nile virus (WNV) strain Sarafend genome were investigated. Short hairpin RNAs targeting Capsid, NS2B and NS4B genes were cloned into pSilencer™ 3.1-H1 neo and designated as pshCapsid, pshNS2B and pshNS4B, respectively. Vero cells that were positively transfected were selected for creating stable cell lines expressing shRNAs constitutively. These cells were subjected to West Nile virus at multiplicity of infection (M.O.I.) of 10. The cells stably transfected with pshCapsid gave the best silencing effect among the three stable cell lines (transfected with pshCapsid, pshNS2B and pshNS4B) at both 12- and 24 h p.i. When compared to the non-transfected WNV-infected cells, pshCapsid stably transfected cells showed more than 4 log10 unit reduction in viral transcripts and greater than 3 log10 unit reduction in virus production. Cells stably transfected with pshNS2B did not exhibit as strong an inhibition when compared to the pshCapsid stably transfected cells having only 2 log10 unit reduction in virus titre. The pshNS4B-stably transfected cells did not suppress WNV replication. Hence, from this study, pshCapsid has the potential to be developed into effective antiviral strategy for WNV infection." @default.
• W2086845471 created "2016-06-24" @default.
• W2086845471 creator A5009580061 @default.
• W2086845471 creator A5053700556 @default.
• W2086845471 creator A5064691389 @default.
• W2086845471 date "2008-08-01" @default.
• W2086845471 modified "2023-09-26" @default.
• W2086845471 title "Inhibition of West Nile virus replication in cells stably transfected with vector-based shRNA expression system" @default.
• W2086845471 cites W1967317528 @default.
• W2086845471 cites W1971791193 @default.
• W2086845471 cites W1980019586 @default.
• W2086845471 cites W1982944995 @default.
• W2086845471 cites W1984006325 @default.
• W2086845471 cites W2019841630 @default.
• W2086845471 cites W2027598863 @default.
• W2086845471 cites W2034912024 @default.
• W2086845471 cites W2060833930 @default.
• W2086845471 cites W2065480841 @default.
• W2086845471 cites W2071171955 @default.
• W2086845471 cites W2087606023 @default.
• W2086845471 cites W2097261962 @default.
• W2086845471 cites W2105414274 @default.
• W2086845471 cites W2110921202 @default.
• W2086845471 cites W2119469827 @default.
• W2086845471 cites W2125057438 @default.
• W2086845471 cites W2144985342 @default.
• W2086845471 cites W2149538815 @default.
• W2086845471 cites W2150247325 @default.
• W2086845471 cites W2158660705 @default.
• W2086845471 cites W2160590732 @default.
• W2086845471 cites W2163412375 @default.
• W2086845471 doi "https://doi.org/10.1016/j.virusres.2008.04.014" @default.
• W2086845471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18514349" @default.
• W2086845471 hasPublicationYear "2008" @default.
• W2086845471 type Work @default.
• W2086845471 sameAs 2086845471 @default.
• W2086845471 citedByCount "16" @default.
• W2086845471 countsByYear W20868454712012 @default.
• W2086845471 countsByYear W20868454712013 @default.
• W2086845471 countsByYear W20868454712014 @default.
• W2086845471 countsByYear W20868454712015 @default.
• W2086845471 countsByYear W20868454712016 @default.
• W2086845471 countsByYear W20868454712017 @default.
• W2086845471 countsByYear W20868454712018 @default.
• W2086845471 countsByYear W20868454712022 @default.
• W2086845471 countsByYear W20868454712023 @default.
• W2086845471 crossrefType "journal-article" @default.
• W2086845471 hasAuthorship W2086845471A5009580061 @default.
• W2086845471 hasAuthorship W2086845471A5053700556 @default.
• W2086845471 hasAuthorship W2086845471A5064691389 @default.
• W2086845471 hasConcept C104317684 @default.
• W2086845471 hasConcept C119056186 @default.
• W2086845471 hasConcept C140704245 @default.
• W2086845471 hasConcept C153911025 @default.
• W2086845471 hasConcept C159047783 @default.
• W2086845471 hasConcept C166703698 @default.
• W2086845471 hasConcept C173396325 @default.
• W2086845471 hasConcept C174510640 @default.
• W2086845471 hasConcept C190232843 @default.
• W2086845471 hasConcept C202878990 @default.
• W2086845471 hasConcept C203233990 @default.
• W2086845471 hasConcept C2522874641 @default.
• W2086845471 hasConcept C32470452 @default.
• W2086845471 hasConcept C32611913 @default.
• W2086845471 hasConcept C40767141 @default.
• W2086845471 hasConcept C54009773 @default.
• W2086845471 hasConcept C54355233 @default.
• W2086845471 hasConcept C67705224 @default.
• W2086845471 hasConcept C81885089 @default.
• W2086845471 hasConcept C86803240 @default.
• W2086845471 hasConcept C89138240 @default.
• W2086845471 hasConceptScore W2086845471C104317684 @default.
• W2086845471 hasConceptScore W2086845471C119056186 @default.
• W2086845471 hasConceptScore W2086845471C140704245 @default.
• W2086845471 hasConceptScore W2086845471C153911025 @default.
• W2086845471 hasConceptScore W2086845471C159047783 @default.
• W2086845471 hasConceptScore W2086845471C166703698 @default.
• W2086845471 hasConceptScore W2086845471C173396325 @default.
• W2086845471 hasConceptScore W2086845471C174510640 @default.
• W2086845471 hasConceptScore W2086845471C190232843 @default.
• W2086845471 hasConceptScore W2086845471C202878990 @default.
• W2086845471 hasConceptScore W2086845471C203233990 @default.
• W2086845471 hasConceptScore W2086845471C2522874641 @default.
• W2086845471 hasConceptScore W2086845471C32470452 @default.
• W2086845471 hasConceptScore W2086845471C32611913 @default.
• W2086845471 hasConceptScore W2086845471C40767141 @default.
• W2086845471 hasConceptScore W2086845471C54009773 @default.
• W2086845471 hasConceptScore W2086845471C54355233 @default.
• W2086845471 hasConceptScore W2086845471C67705224 @default.
• W2086845471 hasConceptScore W2086845471C81885089 @default.
• W2086845471 hasConceptScore W2086845471C86803240 @default.
• W2086845471 hasConceptScore W2086845471C89138240 @default.
• W2086845471 hasFunder F4320320698 @default.
• W2086845471 hasFunder F4320335846 @default.
• W2086845471 hasIssue "2" @default.
• W2086845471 hasLocation W20868454711 @default.
• W2086845471 hasLocation W20868454712 @default.
• W2086845471 hasOpenAccess W2086845471 @default.

• next