FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086884857> ?p ?o ?g. }

• W2086884857 abstract "This report documents the feasibility and advantages of integrating hydrogen/deuterium exchange (HDX) methodology with cyanylation (CN)-based methodology to determine the conformation of cystinyl proteins and intermediates during refolding. The CN-based methodology can be used to trap, identify, and preserve the disulfide structure of a given cystinyl protein folding intermediate, while the HDX methodology can be used to assess other conformational features of the intermediate. Specifically, in this study, CN-based methodology was used to trap a 1-disulfide bond and a 2-disulfide intermediate of long Arg(3) insulin-like growth factor-I (LR(3)IGF-I), which was then exposed to HDX using D(2)O at pD 6.8 and subsequently digested with pepsin before analysis by matrix-assisted laser desorption/ionization mass spectrometry. The HDX results show an increasing degree of secondary and tertiary structure as a function of disulfide bond formation. In addition, the HDX results for two overlapping peptic fragments suggest that a segment of the polypeptide exists in two conformations, which can be distinguished by HDX and pepsin. These results from HDX mass spectrometry are in reasonably good agreement with those from nuclear magnetic resonance studies of native LR(3)IGF-I and IGF-I, in which approximately 5000 times more material was used than in our study. Indications are that the integrated use of HDX and CN-based methodologies will be effective in studying the refolding of cystinyl proteins at the subnanomole level." @default.
• W2086884857 created "2016-06-24" @default.
• W2086884857 creator A5011680939 @default.
• W2086884857 creator A5033684035 @default.
• W2086884857 creator A5040306577 @default.
• W2086884857 creator A5057781839 @default.
• W2086884857 date "2004-08-01" @default.
• W2086884857 modified "2023-09-27" @default.
• W2086884857 title "Integration of hydrogen/deuterium exchange and cyanylation-based methodology for conformational studies of cystinyl proteins" @default.
• W2086884857 cites W1778510571 @default.
• W2086884857 cites W1819660694 @default.
• W2086884857 cites W1966510993 @default.
• W2086884857 cites W1969249762 @default.
• W2086884857 cites W1992653320 @default.
• W2086884857 cites W1996826403 @default.
• W2086884857 cites W1998710115 @default.
• W2086884857 cites W2003396290 @default.
• W2086884857 cites W2013660274 @default.
• W2086884857 cites W2016524985 @default.
• W2086884857 cites W2021942294 @default.
• W2086884857 cites W2031744916 @default.
• W2086884857 cites W2044611093 @default.
• W2086884857 cites W2056979364 @default.
• W2086884857 cites W2060528711 @default.
• W2086884857 cites W2063725210 @default.
• W2086884857 cites W2071228911 @default.
• W2086884857 cites W2094808269 @default.
• W2086884857 cites W2126480217 @default.
• W2086884857 doi "https://doi.org/10.1016/j.ab.2004.03.036" @default.
• W2086884857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15246005" @default.
• W2086884857 hasPublicationYear "2004" @default.
• W2086884857 type Work @default.
• W2086884857 sameAs 2086884857 @default.
• W2086884857 citedByCount "3" @default.
• W2086884857 countsByYear W20868848572012 @default.
• W2086884857 countsByYear W20868848572021 @default.
• W2086884857 crossrefType "journal-article" @default.
• W2086884857 hasAuthorship W2086884857A5011680939 @default.
• W2086884857 hasAuthorship W2086884857A5033684035 @default.
• W2086884857 hasAuthorship W2086884857A5040306577 @default.
• W2086884857 hasAuthorship W2086884857A5057781839 @default.
• W2086884857 hasConcept C109214941 @default.
• W2086884857 hasConcept C121332964 @default.
• W2086884857 hasConcept C162356407 @default.
• W2086884857 hasConcept C167569220 @default.
• W2086884857 hasConcept C185592680 @default.
• W2086884857 hasConcept C27256138 @default.
• W2086884857 hasConcept C2984217123 @default.
• W2086884857 hasConcept C43617362 @default.
• W2086884857 hasConcept C55493867 @default.
• W2086884857 hasConcept C75599170 @default.
• W2086884857 hasConceptScore W2086884857C109214941 @default.
• W2086884857 hasConceptScore W2086884857C121332964 @default.
• W2086884857 hasConceptScore W2086884857C162356407 @default.
• W2086884857 hasConceptScore W2086884857C167569220 @default.
• W2086884857 hasConceptScore W2086884857C185592680 @default.
• W2086884857 hasConceptScore W2086884857C27256138 @default.
• W2086884857 hasConceptScore W2086884857C2984217123 @default.
• W2086884857 hasConceptScore W2086884857C43617362 @default.
• W2086884857 hasConceptScore W2086884857C55493867 @default.
• W2086884857 hasConceptScore W2086884857C75599170 @default.
• W2086884857 hasLocation W20868848571 @default.
• W2086884857 hasLocation W20868848572 @default.
• W2086884857 hasOpenAccess W2086884857 @default.
• W2086884857 hasPrimaryLocation W20868848571 @default.
• W2086884857 hasRelatedWork W1580673954 @default.
• W2086884857 hasRelatedWork W1986904209 @default.
• W2086884857 hasRelatedWork W2056310713 @default.
• W2086884857 hasRelatedWork W2072516608 @default.
• W2086884857 hasRelatedWork W2086884857 @default.
• W2086884857 hasRelatedWork W2318017970 @default.
• W2086884857 hasRelatedWork W2475186594 @default.
• W2086884857 hasRelatedWork W2952737366 @default.
• W2086884857 hasRelatedWork W3043661862 @default.
• W2086884857 hasRelatedWork W4254790889 @default.
• W2086884857 isParatext "false" @default.
• W2086884857 isRetracted "false" @default.
• W2086884857 magId "2086884857" @default.
• W2086884857 workType "article" @default.