Matches in SemOpenAlex for { <https://semopenalex.org/work/W2086942533> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2086942533 endingPage "15387" @default.
- W2086942533 startingPage "15380" @default.
- W2086942533 abstract "Wild-type and mutant forms of bacteriorhodopsin (sbR) from Halobacterium salinarium, produced by Escherichia coli overexpression of a synthetic gene, were reversibly unfolded in 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 3-[(3-cholamidopropyl)dimethylamino]-2-hydroxyl-1-propane (CHAPSO), and sodium dodecyl sulfate (SDS) mixed micelles. To study the effect on protein stability by substitutions on the hydrophobic surface with polar residues, the unfolding behavior of a G113Q, G116Q mutant [sbR(Q2)] was compared to the wild-type sbR [sbR(WT)]. sbR(Q2) was more sensitive to SDS-induced unfolding than sbR(WT) under equilibrium conditions, and kinetic experiments showed that sbR(Q2) was more sensitive to acid-induced denaturation and thermal unfolding than sbR(WT). Since the mutations in sbR(Q2) were on the detergent-embedded hydrophobic surface of sbR, protein destabilization by these mutations supports the concept that the membrane-embedded segments are important for the stability of sbR. Our experiments provide the basis for studying the thermodynamic stability of sbR by evaluating reversible folding and unfolding conditions in DMPC/CHAPSO/SDS mixed micelles." @default.
- W2086942533 created "2016-06-24" @default.
- W2086942533 creator A5039251735 @default.
- W2086942533 creator A5088125571 @default.
- W2086942533 date "1999-10-27" @default.
- W2086942533 modified "2023-09-26" @default.
- W2086942533 title "Probing the Folding and Unfolding of Wild-Type and Mutant Forms of Bacteriorhodopsin in Micellar Solutions: Evaluation of Reversible Unfolding Conditions" @default.
- W2086942533 cites W1499122356 @default.
- W2086942533 cites W1541161575 @default.
- W2086942533 cites W1971739197 @default.
- W2086942533 cites W2035377657 @default.
- W2086942533 cites W2110810808 @default.
- W2086942533 cites W4245084385 @default.
- W2086942533 doi "https://doi.org/10.1021/bi9909039" @default.
- W2086942533 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10563824" @default.
- W2086942533 hasPublicationYear "1999" @default.
- W2086942533 type Work @default.
- W2086942533 sameAs 2086942533 @default.
- W2086942533 citedByCount "59" @default.
- W2086942533 countsByYear W20869425332012 @default.
- W2086942533 countsByYear W20869425332013 @default.
- W2086942533 countsByYear W20869425332014 @default.
- W2086942533 countsByYear W20869425332015 @default.
- W2086942533 countsByYear W20869425332018 @default.
- W2086942533 countsByYear W20869425332019 @default.
- W2086942533 countsByYear W20869425332020 @default.
- W2086942533 countsByYear W20869425332021 @default.
- W2086942533 crossrefType "journal-article" @default.
- W2086942533 hasAuthorship W2086942533A5039251735 @default.
- W2086942533 hasAuthorship W2086942533A5088125571 @default.
- W2086942533 hasConcept C104317684 @default.
- W2086942533 hasConcept C11268172 @default.
- W2086942533 hasConcept C119599485 @default.
- W2086942533 hasConcept C12554922 @default.
- W2086942533 hasConcept C127413603 @default.
- W2086942533 hasConcept C13965031 @default.
- W2086942533 hasConcept C143065580 @default.
- W2086942533 hasConcept C178790620 @default.
- W2086942533 hasConcept C184651966 @default.
- W2086942533 hasConcept C185592680 @default.
- W2086942533 hasConcept C186399102 @default.
- W2086942533 hasConcept C189542057 @default.
- W2086942533 hasConcept C204328495 @default.
- W2086942533 hasConcept C207583985 @default.
- W2086942533 hasConcept C2776545253 @default.
- W2086942533 hasConcept C2776923230 @default.
- W2086942533 hasConcept C2778913249 @default.
- W2086942533 hasConcept C41625074 @default.
- W2086942533 hasConcept C42360764 @default.
- W2086942533 hasConcept C547475151 @default.
- W2086942533 hasConcept C55493867 @default.
- W2086942533 hasConcept C8010536 @default.
- W2086942533 hasConcept C86803240 @default.
- W2086942533 hasConceptScore W2086942533C104317684 @default.
- W2086942533 hasConceptScore W2086942533C11268172 @default.
- W2086942533 hasConceptScore W2086942533C119599485 @default.
- W2086942533 hasConceptScore W2086942533C12554922 @default.
- W2086942533 hasConceptScore W2086942533C127413603 @default.
- W2086942533 hasConceptScore W2086942533C13965031 @default.
- W2086942533 hasConceptScore W2086942533C143065580 @default.
- W2086942533 hasConceptScore W2086942533C178790620 @default.
- W2086942533 hasConceptScore W2086942533C184651966 @default.
- W2086942533 hasConceptScore W2086942533C185592680 @default.
- W2086942533 hasConceptScore W2086942533C186399102 @default.
- W2086942533 hasConceptScore W2086942533C189542057 @default.
- W2086942533 hasConceptScore W2086942533C204328495 @default.
- W2086942533 hasConceptScore W2086942533C207583985 @default.
- W2086942533 hasConceptScore W2086942533C2776545253 @default.
- W2086942533 hasConceptScore W2086942533C2776923230 @default.
- W2086942533 hasConceptScore W2086942533C2778913249 @default.
- W2086942533 hasConceptScore W2086942533C41625074 @default.
- W2086942533 hasConceptScore W2086942533C42360764 @default.
- W2086942533 hasConceptScore W2086942533C547475151 @default.
- W2086942533 hasConceptScore W2086942533C55493867 @default.
- W2086942533 hasConceptScore W2086942533C8010536 @default.
- W2086942533 hasConceptScore W2086942533C86803240 @default.
- W2086942533 hasIssue "46" @default.
- W2086942533 hasLocation W20869425331 @default.
- W2086942533 hasLocation W20869425332 @default.
- W2086942533 hasOpenAccess W2086942533 @default.
- W2086942533 hasPrimaryLocation W20869425331 @default.
- W2086942533 hasRelatedWork W1595027923 @default.
- W2086942533 hasRelatedWork W1987546400 @default.
- W2086942533 hasRelatedWork W1993736519 @default.
- W2086942533 hasRelatedWork W2028014512 @default.
- W2086942533 hasRelatedWork W2035559350 @default.
- W2086942533 hasRelatedWork W2052358099 @default.
- W2086942533 hasRelatedWork W2074283742 @default.
- W2086942533 hasRelatedWork W2086942533 @default.
- W2086942533 hasRelatedWork W2886503850 @default.
- W2086942533 hasRelatedWork W86974628 @default.
- W2086942533 hasVolume "38" @default.
- W2086942533 isParatext "false" @default.
- W2086942533 isRetracted "false" @default.
- W2086942533 magId "2086942533" @default.
- W2086942533 workType "article" @default.