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- W2086971038 abstract "Using high-performance liquid chromatography (HPLC), we showed that spectrophotometric determinations of algal lipophilic pigments are often biased by the choice of extraction solvent. Several commonly used extraction solvents and extraction periods were tested on diverse phytoplankton species isolated from the Neuse River, NC. Extraction solvents included 100% acetone, 90% acetone – 10% distilled deionized water (DDW), 50% acetone – 50% methanol, and 90% methanol – 10% DDW. Extraction efficiencies varied significantly among genera. For the diatom Cyclotella meneghiniana, at least three solvents extracted all lipophilic pigments with equal efficiency, but 50:50 acetone–methanol was clearly superior for the chlorophycean Chlorella vulgaris. A host of differences in extraction efficiencies and extraction times were observed when we compared eukaryotic with prokaryotic (blue-green algae) phytoplankton. We were unable to find a single extraction technique that could consistently provide quantitative and uniform pigment yields from diverse phytoplankton communities. Nevertheless, HPLC techniques can be rapidly and routinely employed to determine appropriate extraction protocols for lipophilic pigments of interest in specific aquatic ecosystems." @default.
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- W2086971038 date "1985-06-01" @default.
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- W2086971038 title "Effective Solvents and Extraction Periods Employed in Phytoplankton Carotenoid and Chlorophyll Determinations" @default.
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- W2086971038 doi "https://doi.org/10.1139/f85-139" @default.
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