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- W2086986076 abstract "A non-radioactive polymerase chain reaction (PCR)-coupled ligase chain reaction (LCR) assay for the specific detection of Listeria monocytogenes was applied to swab samples from metal surfaces that were artificially inoculated with L. monocytogenes. Using a 24 h enrichment this detection system was able to consistently detect 20 cfu L. monocytogenes from a 25 cm2 metal surface, also in the presence of 2 × 105 cfu of Escherichia coli. Detection of non-viable cells resulting from treatment of the inoculated surface with a chlorinating sanitizer was eliminated by the use of a 24 h enrichment step in Listeria enrichment broth." @default.
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- W2086986076 date "1995-02-01" @default.
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- W2086986076 title "Detection of Listeria monocytogenes in surface swabs using a non-radioactive polymerase chain reaction-coupled ligase chain reaction assay" @default.
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- W2086986076 doi "https://doi.org/10.1016/s0740-0020(95)80091-3" @default.
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