FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087010533> ?p ?o ?g. }

• W2087010533 endingPage "692" @default.
• W2087010533 startingPage "690" @default.
• W2087010533 abstract "The mutation status of the immunoglobulin heavy-chain variable-region (IgVH) gene in the affected cells of patients with chronic lymphocytic leukaemia (CLL) is an important prognostic marker (Damle et al, 1999; Hamblin et al, 1999). Mutated IgVH gene is associated with stable disease and long-survival time, while the unmutated gene is associated with rapid disease progression and short-survival time (Keating et al, 2003). The conventional, cell-based, method of determining IgVH gene mutation status involves extraction, amplification and sequencing of the mRNA expressed by the clonal IgVH gene. In some CLL patients, however (those whose disease resides primarily in the lymph nodes or bone marrow and those who have received therapy), few circulating leucocytes are available for mRNA analysis. In addition, in some cases, the presence of reactive polyclonal plasma cells, which express high levels of IgVH mRNA, makes it difficult to isolate pure clonal IgVH mRNA. We previously demonstrated that, as a result of the high turnover of tumour cells relative to non-neoplastic cells, the blood plasma of patients with haematological malignancies is enriched with tumour-specific RNA, DNA (Rogers et al, 2004) and protein (Manshouri et al, 2003), suggesting that plasma might be a reliable source of tumour-specific IgVH mRNA as compared with cells from peripheral blood or bone marrow, where mixed populations of leukaemic and non-leukaemic lymphocytes or plasma cells could hinder sequencing. Here, we investigated that possibility. We collected EDTA-peripheral blood from 49 CLL patients and 20 healthy volunteers <30 years of age. The age limit was imposed to avoid inadvertently including anyone with smouldering CLL. Forty-one of the patients were selected randomly and eight were selected because mRNA extracted from their circulating white cells failed to yield a discrete IgVH band (<10% of the cells were leukaemic). The randomly selected patients had substantial disease with >40% leukaemic cells. Blood samples were collected according to institutional review board-approved protocols. After separating plasma and cells, we extracted total nucleic acid from 0·5 ml of plasma and mRNA from the white cells in 2 ml of blood. IgVH gene mutation status was analysed by performing one-step reverse transcription-polymerase chain reaction (RT-PCR), purifying, sequencing and analysing the PCR product, and then aligning it with the NCBI IgBlast database (http://ncbi.nlm/igblast/). We defined mutation as 2% deviation from the germline sequence. Paired plasma and peripheral blood samples from each of the 41 randomly selected CLL patients yielded identical sequence results (Fig 1A), and 22 pairs (54%) showed mutations. The plasma from the eight patients whose circulating cells failed to yield a discrete IgVH product band due to paucity of leukaemic cells showed discrete bands, and 5 (68%) showed mutations. Plasma and cell mRNA samples from the randomly selected patients showed a discrete IgVH amplification product band while the control plasma samples showed only smeared IgVH bands (Fig 1B). (Smeared bands represent reactive, rather than clonal, IgVH products). (A) Sequence analysis of mRNA extracted from plasma and circulating white cells from the same patient (example 1) yielded identical results. In contrast, the second example (example 2) showed fully readable sequence from plasma, but unreadable sequence from cells. (B) Amplification of IgVH mRNA yielded similar products for plasma (P) and peripheral blood cells (C) from two patients (patient 1 and patient 2) with CLL. Plasma samples from the control subjects (CS) yielded no IgVH amplification products. The bottom panel shows significant IgVH band from plasma, but not from the peripheral blood sample of patient 3 (M, size marker). These data suggest that plasma is a reliable source of tumour-specific RNA in patients with CLL and can be used as an alternative to cells for determining IgVH gene mutation status. This technique would be especially important for patients with few circulating leukaemic cells." @default.
• W2087010533 created "2016-06-24" @default.
• W2087010533 creator A5006104041 @default.
• W2087010533 creator A5027004973 @default.
• W2087010533 creator A5027575595 @default.
• W2087010533 creator A5027800341 @default.
• W2087010533 creator A5031817945 @default.
• W2087010533 creator A5051362583 @default.
• W2087010533 creator A5061285204 @default.
• W2087010533 creator A5066606068 @default.
• W2087010533 creator A5067303243 @default.
• W2087010533 creator A5090435894 @default.
• W2087010533 date "2006-05-16" @default.
• W2087010533 modified "2023-10-16" @default.
• W2087010533 title "Plasma as a source of mRNA for determining IgVH mutation status in patients with chronic lymphocytic leukaemia" @default.
• W2087010533 cites W1243376384 @default.
• W2087010533 cites W1429374962 @default.
• W2087010533 cites W2103006179 @default.
• W2087010533 cites W2134373345 @default.
• W2087010533 cites W2148766690 @default.
• W2087010533 doi "https://doi.org/10.1111/j.1365-2141.2006.06113.x" @default.
• W2087010533 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16704448" @default.
• W2087010533 hasPublicationYear "2006" @default.
• W2087010533 type Work @default.
• W2087010533 sameAs 2087010533 @default.
• W2087010533 citedByCount "8" @default.
• W2087010533 countsByYear W20870105332012 @default.
• W2087010533 countsByYear W20870105332014 @default.
• W2087010533 crossrefType "journal-article" @default.
• W2087010533 hasAuthorship W2087010533A5006104041 @default.
• W2087010533 hasAuthorship W2087010533A5027004973 @default.
• W2087010533 hasAuthorship W2087010533A5027575595 @default.
• W2087010533 hasAuthorship W2087010533A5027800341 @default.
• W2087010533 hasAuthorship W2087010533A5031817945 @default.
• W2087010533 hasAuthorship W2087010533A5051362583 @default.
• W2087010533 hasAuthorship W2087010533A5061285204 @default.
• W2087010533 hasAuthorship W2087010533A5066606068 @default.
• W2087010533 hasAuthorship W2087010533A5067303243 @default.
• W2087010533 hasAuthorship W2087010533A5090435894 @default.
• W2087010533 hasBestOaLocation W20870105331 @default.
• W2087010533 hasConcept C104317684 @default.
• W2087010533 hasConcept C105580179 @default.
• W2087010533 hasConcept C153911025 @default.
• W2087010533 hasConcept C159654299 @default.
• W2087010533 hasConcept C203014093 @default.
• W2087010533 hasConcept C2777938653 @default.
• W2087010533 hasConcept C2778461978 @default.
• W2087010533 hasConcept C2780007613 @default.
• W2087010533 hasConcept C501734568 @default.
• W2087010533 hasConcept C502942594 @default.
• W2087010533 hasConcept C54355233 @default.
• W2087010533 hasConcept C86803240 @default.
• W2087010533 hasConceptScore W2087010533C104317684 @default.
• W2087010533 hasConceptScore W2087010533C105580179 @default.
• W2087010533 hasConceptScore W2087010533C153911025 @default.
• W2087010533 hasConceptScore W2087010533C159654299 @default.
• W2087010533 hasConceptScore W2087010533C203014093 @default.
• W2087010533 hasConceptScore W2087010533C2777938653 @default.
• W2087010533 hasConceptScore W2087010533C2778461978 @default.
• W2087010533 hasConceptScore W2087010533C2780007613 @default.
• W2087010533 hasConceptScore W2087010533C501734568 @default.
• W2087010533 hasConceptScore W2087010533C502942594 @default.
• W2087010533 hasConceptScore W2087010533C54355233 @default.
• W2087010533 hasConceptScore W2087010533C86803240 @default.
• W2087010533 hasIssue "6" @default.
• W2087010533 hasLocation W20870105331 @default.
• W2087010533 hasLocation W20870105332 @default.
• W2087010533 hasOpenAccess W2087010533 @default.
• W2087010533 hasPrimaryLocation W20870105331 @default.
• W2087010533 hasRelatedWork W2072479821 @default.
• W2087010533 hasRelatedWork W2076631739 @default.
• W2087010533 hasRelatedWork W2110529460 @default.
• W2087010533 hasRelatedWork W2409919335 @default.
• W2087010533 hasRelatedWork W2751803569 @default.
• W2087010533 hasRelatedWork W3014999046 @default.
• W2087010533 hasRelatedWork W3098443183 @default.
• W2087010533 hasRelatedWork W322288987 @default.
• W2087010533 hasRelatedWork W4386277455 @default.
• W2087010533 hasRelatedWork W4386544784 @default.
• W2087010533 hasVolume "133" @default.
• W2087010533 isParatext "false" @default.
• W2087010533 isRetracted "false" @default.
• W2087010533 magId "2087010533" @default.
• W2087010533 workType "article" @default.