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• W2087025538 abstract "Plasminogen activator inhibitor type 2 (PAI-2) mRNA and antigen levels are synergistically induced in HT-1080 fibrosarcoma cells when treated with a combination of tumor necrosis factor (TNF) and phorbol 12-myristate 13-acetate (PMA). Here we demonstrate that this effect is not fully reflected at the level of gene transcription, suggesting a contribution of post-transcriptional events in this induction. Insertion of the 3′-untranslated region (3′-UTR) of PAI-2 mRNA into the 3′-UTR of a rabbit β-globin reporter gene reduces β-globin-PAI-2 chimeric mRNA expression in stably transfected cells. The region within the PAI-2 3′-UTR responsible for this effect is located within the 368-nucleotide sequence preceding the poly(A) tail, a segment that includes a nonameric UUAUUUAUU motif. Mutagenesis of this element abolishes the PAI-2 3′-UTR destabilizing effect, revealing a functional role for this motif. TNF and PMA co-treatment of transfected cells increases β-globin-PAI-2 chimeric mRNA expression 3-4-fold, indicating that the inherently unstable 3′-UTR of PAI-2 mRNA can become stabilized in response to TNF and PMA. Our results indicate that induction of PAI-2 gene expression by TNF and PMA involves both direct transcription as well as mRNA stabilization, the latter involving an AU-rich nonameric motif in the 3′-UTR. Plasminogen activator inhibitor type 2 (PAI-2) mRNA and antigen levels are synergistically induced in HT-1080 fibrosarcoma cells when treated with a combination of tumor necrosis factor (TNF) and phorbol 12-myristate 13-acetate (PMA). Here we demonstrate that this effect is not fully reflected at the level of gene transcription, suggesting a contribution of post-transcriptional events in this induction. Insertion of the 3′-untranslated region (3′-UTR) of PAI-2 mRNA into the 3′-UTR of a rabbit β-globin reporter gene reduces β-globin-PAI-2 chimeric mRNA expression in stably transfected cells. The region within the PAI-2 3′-UTR responsible for this effect is located within the 368-nucleotide sequence preceding the poly(A) tail, a segment that includes a nonameric UUAUUUAUU motif. Mutagenesis of this element abolishes the PAI-2 3′-UTR destabilizing effect, revealing a functional role for this motif. TNF and PMA co-treatment of transfected cells increases β-globin-PAI-2 chimeric mRNA expression 3-4-fold, indicating that the inherently unstable 3′-UTR of PAI-2 mRNA can become stabilized in response to TNF and PMA. Our results indicate that induction of PAI-2 gene expression by TNF and PMA involves both direct transcription as well as mRNA stabilization, the latter involving an AU-rich nonameric motif in the 3′-UTR." @default.
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• W2087025538 date "1996-10-01" @default.
• W2087025538 modified "2023-10-17" @default.
• W2087025538 title "Plasminogen Activator Inhibitor Type 2 Gene Induction by Tumor Necrosis Factor and Phorbol Ester Involves Transcriptional and Post-transcriptional Events" @default.
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• W2087025538 doi "https://doi.org/10.1074/jbc.271.42.26074" @default.
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