FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087029178> ?p ?o ?g. }

• W2087029178 endingPage "32217" @default.
• W2087029178 startingPage "32209" @default.
• W2087029178 abstract "Fructansucrases, members of glycoside hydrolase family 68, catalyze both sucrose hydrolysis and the polymerization of fructose to β-d-fructofuranose polymers. The resulting fructan polymers are distinguished by the nature of the glycosidic bond: inulin (β-(2–1)-fructofuranose) and levan (β-(2–6)-fructofuranose). In this study we demonstrate that <i>Zymomonas mobilis</i> levansucrase exists in two active forms, depending on the pH and ionic strength. At pH values above 7.0, the enzyme is mainly a dimer, whereas at pH values below 6.0, the protein forms well ordered microfibrils that precipitate out of the solution. These two forms are readily interchangeable simply by changing the pH. Surprisingly the manner in which the enzyme is arranged strongly affects its product specificity and kinetic properties. At pH values above 7.0, the activity of the enzyme as a dimer is mainly sucrose hydrolysis and the synthesis of short fructosaccharides (degree of polymerization, 3). At pH values below 6.0, in its microfibril form, the enzyme catalyzes almost exclusively the synthesis of levan (a degree of polymerization greater than 20,000). This difference in product specificity appears to depend on the form of the enzyme, dimer <i>versus</i> microfibril, and not directly on the pH. Images made by negative stain transmission electron microscopy reveal that the enzyme forms a very ordered structure of long fibrils that appear to be composed of repeating rings of six to eight protein units. A single amino acid replacement of H296R abolished the ability of the enzyme to form microfibrils with organized fibril networks and to synthesize levan at pH 6.0." @default.
• W2087029178 created "2016-06-24" @default.
• W2087029178 creator A5011337144 @default.
• W2087029178 creator A5012008977 @default.
• W2087029178 creator A5045973927 @default.
• W2087029178 creator A5055813206 @default.
• W2087029178 creator A5080485984 @default.
• W2087029178 creator A5087124940 @default.
• W2087029178 date "2008-11-01" @default.
• W2087029178 modified "2023-10-14" @default.
• W2087029178 title "Two Active Forms of Zymomonas mobilis Levansucrase" @default.
• W2087029178 cites W152058491 @default.
• W2087029178 cites W1790041928 @default.
• W2087029178 cites W1806434068 @default.
• W2087029178 cites W1968585339 @default.
• W2087029178 cites W1970397189 @default.
• W2087029178 cites W1987416060 @default.
• W2087029178 cites W2001038197 @default.
• W2087029178 cites W2001843496 @default.
• W2087029178 cites W2008232993 @default.
• W2087029178 cites W2008852014 @default.
• W2087029178 cites W2009580263 @default.
• W2087029178 cites W2010050098 @default.
• W2087029178 cites W2024908520 @default.
• W2087029178 cites W2031049554 @default.
• W2087029178 cites W2034537831 @default.
• W2087029178 cites W2038124483 @default.
• W2087029178 cites W2055141813 @default.
• W2087029178 cites W2060308945 @default.
• W2087029178 cites W2064062887 @default.
• W2087029178 cites W2077854669 @default.
• W2087029178 cites W2079737908 @default.
• W2087029178 cites W2085641407 @default.
• W2087029178 cites W2097184433 @default.
• W2087029178 cites W2111051779 @default.
• W2087029178 cites W2113964973 @default.
• W2087029178 cites W2126936421 @default.
• W2087029178 cites W2143053628 @default.
• W2087029178 cites W2143430331 @default.
• W2087029178 cites W2152301430 @default.
• W2087029178 cites W2162191565 @default.
• W2087029178 cites W2950469006 @default.
• W2087029178 doi "https://doi.org/10.1074/jbc.m805985200" @default.
• W2087029178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18809687" @default.
• W2087029178 hasPublicationYear "2008" @default.
• W2087029178 type Work @default.
• W2087029178 sameAs 2087029178 @default.
• W2087029178 citedByCount "60" @default.
• W2087029178 countsByYear W20870291782012 @default.
• W2087029178 countsByYear W20870291782013 @default.
• W2087029178 countsByYear W20870291782014 @default.
• W2087029178 countsByYear W20870291782015 @default.
• W2087029178 countsByYear W20870291782016 @default.
• W2087029178 countsByYear W20870291782017 @default.
• W2087029178 countsByYear W20870291782018 @default.
• W2087029178 countsByYear W20870291782019 @default.
• W2087029178 countsByYear W20870291782020 @default.
• W2087029178 countsByYear W20870291782021 @default.
• W2087029178 countsByYear W20870291782022 @default.
• W2087029178 countsByYear W20870291782023 @default.
• W2087029178 crossrefType "journal-article" @default.
• W2087029178 hasAuthorship W2087029178A5011337144 @default.
• W2087029178 hasAuthorship W2087029178A5012008977 @default.
• W2087029178 hasAuthorship W2087029178A5045973927 @default.
• W2087029178 hasAuthorship W2087029178A5055813206 @default.
• W2087029178 hasAuthorship W2087029178A5080485984 @default.
• W2087029178 hasAuthorship W2087029178A5087124940 @default.
• W2087029178 hasBestOaLocation W20870291781 @default.
• W2087029178 hasConcept C150487720 @default.
• W2087029178 hasConcept C178790620 @default.
• W2087029178 hasConcept C181199279 @default.
• W2087029178 hasConcept C185592680 @default.
• W2087029178 hasConcept C191516044 @default.
• W2087029178 hasConcept C2776455902 @default.
• W2087029178 hasConcept C2776970464 @default.
• W2087029178 hasConcept C2777272437 @default.
• W2087029178 hasConcept C2778597767 @default.
• W2087029178 hasConcept C2778886173 @default.
• W2087029178 hasConcept C2779251873 @default.
• W2087029178 hasConcept C2779546866 @default.
• W2087029178 hasConcept C2780161600 @default.
• W2087029178 hasConcept C2780349071 @default.
• W2087029178 hasConcept C2780604136 @default.
• W2087029178 hasConcept C44228677 @default.
• W2087029178 hasConcept C521977710 @default.
• W2087029178 hasConcept C523546767 @default.
• W2087029178 hasConcept C54355233 @default.
• W2087029178 hasConcept C55493867 @default.
• W2087029178 hasConcept C65183824 @default.
• W2087029178 hasConcept C71240020 @default.
• W2087029178 hasConcept C73583747 @default.
• W2087029178 hasConcept C86803240 @default.
• W2087029178 hasConcept C94412978 @default.
• W2087029178 hasConceptScore W2087029178C150487720 @default.
• W2087029178 hasConceptScore W2087029178C178790620 @default.
• W2087029178 hasConceptScore W2087029178C181199279 @default.
• W2087029178 hasConceptScore W2087029178C185592680 @default.
• W2087029178 hasConceptScore W2087029178C191516044 @default.

• next