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- W2087030660 abstract "Detection of minimal residual disease and relapse remain major problems in chronic myelogenous leukemia (CML) patients following bone marrow transplantation (BMT). In order to disclose the 9;22 Philadelphia translocation, we used a fluorescence in situ hybridization (FISH) technique. BCR and ABL gene fragments were used as probes for the detection of the BCR/ABL fusion product in peripheral blood and bone marrow cells from 11 CML patients in which 5 were post-BMT. The sensitivity and specificity of this approach were compared to conventional cytogenetic and polymerase chain reaction (PCR) methods. FISH demonstrated a high degree of sensitivity (1%) for the detection of the BCR/ABL translocation in these patients. A linear correlation was found between FISH detection of the BCR/ABL fusion product and routine chromosomal analysis (r = 0.995; p < 0.001). Detection of the BCR/ABL signal by FISH was observed in all patients showing a positive PCR signal. A significant reduction in BCR/ABL signal was observed post-transplant (p < 0.001). However, the BCR/ABL translocation was detected in four of five transplanted patients immediately (0.75–2.5 months) following transplant and was found in patients with a low expression of the translocation." @default.
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- W2087030660 date "1994-08-01" @default.
- W2087030660 modified "2023-09-23" @default.
- W2087030660 title "Detection of minimal residual disease state in chronic myelogenous leukemia patients using fluorescence in situ hybridization" @default.
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- W2087030660 doi "https://doi.org/10.1016/0165-4608(94)90073-6" @default.
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