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- W2087039006 abstract "To screen siRNAs for effectively inhibiting the replication of porcine reproductive and respiratory syndrome virus (PRRSV). Four pairs of siRNA targeting Nsp9 gene of PRRSV and one non-efficient pair used as control were designed, synthesized and cloned into pSilencer4.1-CMV neo, designated as pSi-294, pSi-367, pSi-409, pSi-1488, pSi-Ctr. The recombinant plasmids were transfected into Marc-145 cells and infected with PRRSV 24h post transfection. Subsequently, IFA, real-time PCR, TCID50 and western blot were used for evaluating the inhibitory effect of the siRNA. IFA and western-blot results showed that pSi-294, pSi-1488 can effectively inhibit the expression of Nsp9 and M protein of PRRSV, real-time PCR result showed that the expression of Nsp9 gene were decreased from 86.56% to 93.66% compared to the negative control. siRNAs can be used as candidates for basic research of PRRSV." @default.
- W2087039006 created "2016-06-24" @default.
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- W2087039006 date "2014-12-01" @default.
- W2087039006 modified "2023-10-18" @default.
- W2087039006 title "Inhibition of porcine reproductive and respiratory syndrome virus by specific siRNA targeting Nsp9 gene" @default.
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- W2087039006 doi "https://doi.org/10.1016/j.meegid.2014.08.008" @default.
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