Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087083313> ?p ?o ?g. }
- W2087083313 endingPage "730" @default.
- W2087083313 startingPage "723" @default.
- W2087083313 abstract "This paper studies the synthesis of structured triacylglycerols (STAGs), rich in polyunsaturated fatty acids (PUFAs) by a two-step enzymatic process: (i) alcoholysis of fish oils (cod liver and tuna oils) with ethanol to obtain 2-monoacylglycerols (2-MAGs), catalyzed by 1,3 specific lipases and (ii) esterification of these 2-MAGs with caprylic acid (CA, 8:0), also catalyzed by a 1,3 specific lipase, to produce STAGs of structure CA–PUFA–CA. As regards the alcoholysis reaction, three factors have been studied: the influence of the type of lipase used (lipase D from Rhizopus oryzae, immobilized on Accurel MP1000, and Novozym 435 from Candida antarctica), the operational mode of a stirred tank reactor (STR operating in discontinuous and continuous mode) and the intensity of treatment (IOT = lipase amount × reaction time/oil amount). Although higher 2-MAG yields were obtained with lipase D, Novozym 435 was selected due to its greater stability in the operational conditions. The highest 2-MAG yield (63%) was attained in the STR operating in discontinuous mode at an IOT of 1 g lipase × h g oil−1 (at higher IOT the 2-MAGs were degraded to glycerol). This system was scaled up to 100 times the initial volume, achieving a similar yield (65%) at the same IOT. The 2-MAGs in the final alcoholysis reaction mixture were separated from ethyl esters by solvent extraction using solvents of low toxicity (ethanol and hexane); the 2-MAG recovery yield was over 90% and the purity was approximately 87–90%. Regarding the esterification of the 2-MAGs, the following factors were studied: the influence of the lipase type used, the presence or absence of solvent (hexane) and the reaction time or intensity of treatment (IOT = lipase amount × reaction time/2-MAG amount). Of the five lipases tested, the highest STAG percentages (over 90%) were attained with lipases D and DF, immobilized on Accurel MP1000. These STAGs contain 64% CA, of which 98% is at positions 1 and 3. Position 2 contains 5% CA and 45% PUFAs, which means that all the PUFAs that were located at position 2 in the original oil remain in that position in the final STAGs. The lipase D immobilized on Accurel MP1000 is stable in the operational conditions used in the esterification reaction. Finally the purification of STAGs was carried out by neutralization of free fatty acids with hydroethanolic solution of KOH and extraction of STAGs with hexane. By this method purity was over 95% and separation yields were about 80%." @default.
- W2087083313 created "2016-06-24" @default.
- W2087083313 creator A5034518477 @default.
- W2087083313 creator A5049597944 @default.
- W2087083313 creator A5055535399 @default.
- W2087083313 creator A5062563503 @default.
- W2087083313 creator A5070936104 @default.
- W2087083313 date "2009-07-01" @default.
- W2087083313 modified "2023-10-16" @default.
- W2087083313 title "Synthesis of structured lipids by two enzymatic steps: Ethanolysis of fish oils and esterification of 2-monoacylglycerols" @default.
- W2087083313 cites W1933801203 @default.
- W2087083313 cites W1966569340 @default.
- W2087083313 cites W1982524823 @default.
- W2087083313 cites W1989848878 @default.
- W2087083313 cites W1998020276 @default.
- W2087083313 cites W2008673130 @default.
- W2087083313 cites W2009267782 @default.
- W2087083313 cites W2010002118 @default.
- W2087083313 cites W2014381213 @default.
- W2087083313 cites W2018379572 @default.
- W2087083313 cites W2018918067 @default.
- W2087083313 cites W2037471240 @default.
- W2087083313 cites W2042904981 @default.
- W2087083313 cites W2047886710 @default.
- W2087083313 cites W2051741802 @default.
- W2087083313 cites W2055559957 @default.
- W2087083313 cites W2056565650 @default.
- W2087083313 cites W2064834378 @default.
- W2087083313 cites W2069769258 @default.
- W2087083313 cites W2076233204 @default.
- W2087083313 cites W2084869231 @default.
- W2087083313 cites W2086590012 @default.
- W2087083313 cites W2088342377 @default.
- W2087083313 cites W2092476733 @default.
- W2087083313 cites W2107174188 @default.
- W2087083313 cites W2107928904 @default.
- W2087083313 cites W2116959209 @default.
- W2087083313 cites W2126689542 @default.
- W2087083313 cites W2139497665 @default.
- W2087083313 cites W2171145615 @default.
- W2087083313 cites W42433123 @default.
- W2087083313 doi "https://doi.org/10.1016/j.procbio.2009.03.002" @default.
- W2087083313 hasPublicationYear "2009" @default.
- W2087083313 type Work @default.
- W2087083313 sameAs 2087083313 @default.
- W2087083313 citedByCount "50" @default.
- W2087083313 countsByYear W20870833132012 @default.
- W2087083313 countsByYear W20870833132013 @default.
- W2087083313 countsByYear W20870833132014 @default.
- W2087083313 countsByYear W20870833132015 @default.
- W2087083313 countsByYear W20870833132016 @default.
- W2087083313 countsByYear W20870833132017 @default.
- W2087083313 countsByYear W20870833132018 @default.
- W2087083313 countsByYear W20870833132019 @default.
- W2087083313 countsByYear W20870833132020 @default.
- W2087083313 countsByYear W20870833132021 @default.
- W2087083313 countsByYear W20870833132022 @default.
- W2087083313 countsByYear W20870833132023 @default.
- W2087083313 crossrefType "journal-article" @default.
- W2087083313 hasAuthorship W2087083313A5034518477 @default.
- W2087083313 hasAuthorship W2087083313A5049597944 @default.
- W2087083313 hasAuthorship W2087083313A5055535399 @default.
- W2087083313 hasAuthorship W2087083313A5062563503 @default.
- W2087083313 hasAuthorship W2087083313A5070936104 @default.
- W2087083313 hasConcept C100544194 @default.
- W2087083313 hasConcept C134121241 @default.
- W2087083313 hasConcept C178790620 @default.
- W2087083313 hasConcept C181199279 @default.
- W2087083313 hasConcept C185592680 @default.
- W2087083313 hasConcept C19038510 @default.
- W2087083313 hasConcept C191897082 @default.
- W2087083313 hasConcept C192562407 @default.
- W2087083313 hasConcept C2777077568 @default.
- W2087083313 hasConcept C2778541762 @default.
- W2087083313 hasConcept C2779542420 @default.
- W2087083313 hasConcept C2779697368 @default.
- W2087083313 hasConcept C2779946292 @default.
- W2087083313 hasConcept C2780161600 @default.
- W2087083313 hasConcept C2780471494 @default.
- W2087083313 hasConcept C2780881558 @default.
- W2087083313 hasConcept C2781066024 @default.
- W2087083313 hasConcept C2909208804 @default.
- W2087083313 hasConcept C43617362 @default.
- W2087083313 hasConcept C505870484 @default.
- W2087083313 hasConcept C543025807 @default.
- W2087083313 hasConcept C86803240 @default.
- W2087083313 hasConceptScore W2087083313C100544194 @default.
- W2087083313 hasConceptScore W2087083313C134121241 @default.
- W2087083313 hasConceptScore W2087083313C178790620 @default.
- W2087083313 hasConceptScore W2087083313C181199279 @default.
- W2087083313 hasConceptScore W2087083313C185592680 @default.
- W2087083313 hasConceptScore W2087083313C19038510 @default.
- W2087083313 hasConceptScore W2087083313C191897082 @default.
- W2087083313 hasConceptScore W2087083313C192562407 @default.
- W2087083313 hasConceptScore W2087083313C2777077568 @default.
- W2087083313 hasConceptScore W2087083313C2778541762 @default.
- W2087083313 hasConceptScore W2087083313C2779542420 @default.
- W2087083313 hasConceptScore W2087083313C2779697368 @default.