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- W2087126851 abstract "Free-flow electrophoresis techniques have been applied for separations in various areas of chemistry and biochemistry. Here we focus on the generation of a free-flow electrophoresis chip and direct monitoring of the separation of different molecules in the separation bed of the miniaturized chip. We demonstrate a fast and efficient way to generate a low-cost micro-free-flow electrophoresis (μFFE) chip with a filling capacity of 9.5 μL based on a multi-lamination technique. Separating webs realized by two transfer-adhesive tapes avoid the problem of gas bubbles entering the separation area. The chip is characterized by isoelectric focusing markers (IEF markers). The functionality of the chip is demonstrated by free-flow isoelectric focusing (FFIEF) of the proteins BSA (bovine serum albumin) and avidin and a single-stranded DNA (ssDNA) fragment in the pH range 3 to 10. The separation voltage ranges between 167 V cm−1 and 422 V cm−1, depending on the application." @default.
- W2087126851 created "2016-06-24" @default.
- W2087126851 creator A5034347181 @default.
- W2087126851 creator A5047222708 @default.
- W2087126851 creator A5078265978 @default.
- W2087126851 date "2011-09-13" @default.
- W2087126851 modified "2023-10-03" @default.
- W2087126851 title "Generation of a miniaturized free-flow electrophoresis chip based on a multi-lamination technique—isoelectric focusing of proteins and a single-stranded DNA fragment" @default.
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- W2087126851 doi "https://doi.org/10.1007/s00216-011-5353-0" @default.
- W2087126851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21912834" @default.
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