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- W2087149247 abstract "Small circular plasmids containing replication origins and, in some cases, centromeres, can replicate autonomously in the nuclei of all tested yeast species. Because this autonomous replication is dependent on the replication origin within the plasmid, measurements of the efficiency of autonomous replication (by the methods summarized here) permit evaluation of the effects of mutations on origin function. Although alternative methods are available for genetic characterization of replication origins in other organisms, the simplicity of the autonomous replication assay in yeasts has permitted development of the deepest understanding to date of eukaryotic replication origin structure. This information has come primarily from studies with Saccharomyces cerevisiae. However, there are many other yeast species, each with its own variety of replication origins. Use of the methods summarized here to characterize origins in other yeast species is likely to provide additional insights into eukaryotic replication origin structure." @default.
- W2087149247 created "2016-06-24" @default.
- W2087149247 creator A5063936024 @default.
- W2087149247 date "1999-07-01" @default.
- W2087149247 modified "2023-10-14" @default.
- W2087149247 title "Genetic Methods for Characterizing the cis-Acting Components of Yeast DNA Replication Origins" @default.
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- W2087149247 doi "https://doi.org/10.1006/meth.1999.0792" @default.
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