FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087150905> ?p ?o ?g. }

• W2087150905 endingPage "182" @default.
• W2087150905 startingPage "175" @default.
• W2087150905 abstract "Examination of prostasomes, isolated from human seminal plasma, showed that there was very little remaining paranitrophenylphosphatase activity when assayed in the presence of 10 mmol/l of tartrate and 2 mmol/l of levamisole. Under these conditions it was possible to study the prostasome membrane-bound 5′-nucleotidase activity, which was unaffected by these two inhibitors. The activity was considered to be located at the external surface of the prostasome membrane and a 50–60% increase in activity was obtained by the addition of 0.05% Triton X-100. The prostasome membrane-linked 5′-nucleotidase readily hydrolysed 5′-AMP. Two other 5′-nucleoside monophosphates, 5′-IMP and 5′-GMP, were also hydrolysed, but more slowly; 2′- or 3′-AMP were practically not attacked. The prostasome membrane-linked 5′-nucleotidase obeyed Michaelis-Menten kinetics. Apparent Km for 5′-AMP was 11.2 ± 2.1 μmol/l and Vmax 64.7 ± 11.4 nmol/mg protein/min. These figures were somewhat changed in presence of 0.05% Triton X-100, the Km value being reduced by 30% and the Vmax value increased by 60%. Adenosine 5′ (α, β methylene) diphosphate (100 μmol/l), Ni2+ (10 mmol/l) and concanavalin A (20 μg/ml) were all potent inhibitors of the prostasome membrane-linked 5′-nucleotidase." @default.
• W2087150905 created "2016-06-24" @default.
• W2087150905 creator A5002055709 @default.
• W2087150905 creator A5023183087 @default.
• W2087150905 date "1993-07-01" @default.
• W2087150905 modified "2023-10-17" @default.
• W2087150905 title "Characteristics of membrane-bound 5′-nucleotidase on human prostasomes" @default.
• W2087150905 cites W125136280 @default.
• W2087150905 cites W1775749144 @default.
• W2087150905 cites W1974040702 @default.
• W2087150905 cites W1980009977 @default.
• W2087150905 cites W1980398704 @default.
• W2087150905 cites W1988760264 @default.
• W2087150905 cites W1989689640 @default.
• W2087150905 cites W2002470184 @default.
• W2087150905 cites W2012252491 @default.
• W2087150905 cites W2016686298 @default.
• W2087150905 cites W2027984759 @default.
• W2087150905 cites W2032892371 @default.
• W2087150905 cites W2034146890 @default.
• W2087150905 cites W2036754828 @default.
• W2087150905 cites W2043055469 @default.
• W2087150905 cites W2059019596 @default.
• W2087150905 cites W2073197896 @default.
• W2087150905 cites W2082348097 @default.
• W2087150905 cites W2088022170 @default.
• W2087150905 cites W2094990615 @default.
• W2087150905 cites W2152145468 @default.
• W2087150905 cites W2231111026 @default.
• W2087150905 cites W2321272748 @default.
• W2087150905 cites W2339579100 @default.
• W2087150905 cites W2357738814 @default.
• W2087150905 cites W2409738968 @default.
• W2087150905 cites W2755175530 @default.
• W2087150905 cites W45164427 @default.
• W2087150905 doi "https://doi.org/10.1016/0009-8981(93)90150-3" @default.
• W2087150905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8222268" @default.
• W2087150905 hasPublicationYear "1993" @default.
• W2087150905 type Work @default.
• W2087150905 sameAs 2087150905 @default.
• W2087150905 citedByCount "21" @default.
• W2087150905 countsByYear W20871509052012 @default.
• W2087150905 countsByYear W20871509052013 @default.
• W2087150905 countsByYear W20871509052014 @default.
• W2087150905 countsByYear W20871509052016 @default.
• W2087150905 countsByYear W20871509052020 @default.
• W2087150905 crossrefType "journal-article" @default.
• W2087150905 hasAuthorship W2087150905A5002055709 @default.
• W2087150905 hasAuthorship W2087150905A5023183087 @default.
• W2087150905 hasConcept C124815738 @default.
• W2087150905 hasConcept C153911025 @default.
• W2087150905 hasConcept C181199279 @default.
• W2087150905 hasConcept C185592680 @default.
• W2087150905 hasConcept C18880352 @default.
• W2087150905 hasConcept C19869320 @default.
• W2087150905 hasConcept C202751555 @default.
• W2087150905 hasConcept C2776543447 @default.
• W2087150905 hasConcept C2776991684 @default.
• W2087150905 hasConcept C2777913276 @default.
• W2087150905 hasConcept C41625074 @default.
• W2087150905 hasConcept C43617362 @default.
• W2087150905 hasConcept C55493867 @default.
• W2087150905 hasConcept C86803240 @default.
• W2087150905 hasConcept C94412978 @default.
• W2087150905 hasConceptScore W2087150905C124815738 @default.
• W2087150905 hasConceptScore W2087150905C153911025 @default.
• W2087150905 hasConceptScore W2087150905C181199279 @default.
• W2087150905 hasConceptScore W2087150905C185592680 @default.
• W2087150905 hasConceptScore W2087150905C18880352 @default.
• W2087150905 hasConceptScore W2087150905C19869320 @default.
• W2087150905 hasConceptScore W2087150905C202751555 @default.
• W2087150905 hasConceptScore W2087150905C2776543447 @default.
• W2087150905 hasConceptScore W2087150905C2776991684 @default.
• W2087150905 hasConceptScore W2087150905C2777913276 @default.
• W2087150905 hasConceptScore W2087150905C41625074 @default.
• W2087150905 hasConceptScore W2087150905C43617362 @default.
• W2087150905 hasConceptScore W2087150905C55493867 @default.
• W2087150905 hasConceptScore W2087150905C86803240 @default.
• W2087150905 hasConceptScore W2087150905C94412978 @default.
• W2087150905 hasIssue "1-2" @default.
• W2087150905 hasLocation W20871509051 @default.
• W2087150905 hasLocation W20871509052 @default.
• W2087150905 hasOpenAccess W2087150905 @default.
• W2087150905 hasPrimaryLocation W20871509051 @default.
• W2087150905 hasRelatedWork W1430790132 @default.
• W2087150905 hasRelatedWork W1841130358 @default.
• W2087150905 hasRelatedWork W2103223904 @default.
• W2087150905 hasRelatedWork W2106938624 @default.
• W2087150905 hasRelatedWork W2332243498 @default.
• W2087150905 hasRelatedWork W2401499450 @default.
• W2087150905 hasRelatedWork W2409967943 @default.
• W2087150905 hasRelatedWork W2418305927 @default.
• W2087150905 hasRelatedWork W45164427 @default.
• W2087150905 hasRelatedWork W961682450 @default.
• W2087150905 hasVolume "216" @default.
• W2087150905 isParatext "false" @default.
• W2087150905 isRetracted "false" @default.
• W2087150905 magId "2087150905" @default.

• next